Article

Hepatoprotective activity of extracts from Pergularia daemia Forsk. against carbon tetrachloride-induced toxicity in rats. S.V.Suresh Kumar and S.H.Mishra. Pharmacognosy Magazine, 3 (11), 2007, 187-191

Authors:
To read the full-text of this research, you can request a copy directly from the author.

No full-text available

Request Full-text Paper PDF

To read the full-text of this research,
you can request a copy directly from the author.

... Blood samples were collected in microcentrifuge tubes from the individual animal by a retro-orbital puncture on 7 th day. The blood samples were centrifuged at 2500 rpm for 10 min and serum was obtained (Kumar and Mishra, 2007). Antibody levels were determined by the hemagglutination technique. ...
... Circulation of immune cells is essential for maintaining an effective immune defense network. The TLC was found to be increased in the extracts immunonized group, which may be due to the fall in the corticosterone levels and increased glucocorticoids levels that affect the circulation pattern of immune cells [15,16]. This might be due to the upregulation of the β2 integrins, present on the surface of the neutrophils through which they adhere firmly to the nylon fibres [17]. ...
Article
Full-text available
Objective: The present study was undertaken to evaluate the immunomodulatory activity of the alcoholic extracts of Ruta graveolens leaves in vivo. Methods: Immunomodulatory activity was determined by neutrophil adhesion test, phagocytic activity, haemagglutinating antibody (HA) titre and delayed-type hypersensitivity (DTH) response. Results: Oral administration of the extracts (50, 100, 150 and 200 mg/kg bw, p. o) evoked a significant (**P<0.01) increased in percent of neutrophil adhesion to nylon fibers. Both the extracts were also increased the antibody titre in a dose-dependent manner. Oral administration of both the compound significantly (**P<0.01) potentiated delayed type hypersensitivity reaction induced by sheep red blood cells and response towards phagocytosis in carbon clearance assay. In this study indicated that leaves extracts possesses potent immune-modulatory activity in a concentration-dependent manner. The response was statistically significant when compared with the control (*P<0.05, **P<0.01). Conclusion: The study stated that Ruta graveolens leaves extracts showed a significant stimulation of the cell-mediated immunity as well as humoral immunity. Further investigations are required to determine its active constituents and also its mechanism of action.
... Decreased the level of total protein and albumin content in QILE treated animals reflects the hepatoprotective activity of extract as compared to ethanol-treated animals. The elevation in the total protein (TP) and albumin is demonstrating the initial damage produced in ethanol-treated animals due to leakage of nitrogenous substances (non-protein and protein) on destruction of cells; this leads to impairment in protein synthesis and it transamination [26]. ...
Article
Full-text available
Objective: This study was undertaken to investigate the hepatoprotective and antioxidant activity of Quercus ilex leaves extract (QILE) on ethanol-induced toxicity in Wistar rats. Methods: Hepatotoxicity was induced by administering ethanol (40%) at a dose of 7.9 gm/kg/day; p. o. (1:1 of ethanol in olive oil) for 28 d. Silymarin 100 mg/kg/day; p. o. was used as a standard drug. The whole study was divided into a prophylactic and curative study. In the prophylactic study, the Silymarin and QILE (test drug) 100, 200, and 400 mg/kg Body Weight(BW) given orally one hour before administration of 40% ethanol administration for 28 d. In the curative study, 7 d of treatment of Silymarin and QILE 200 and 400 mg/kg BW was given orally after 28 d of ethanol administration to different groups. Results: Hepatoprotectivity was confirmed by the highly significantly (p<0.001) restoration of elevated biochemical parameters like SGPT, SGOT, ALP, TB, and highly significantly (p<0.001) depleted Albumin and Total protein levels by 200 mg/kg BW QILE in comparison to the positive control group. QILE 200 mg/kg highly significantly (p<0.001) raised the antioxidants by draining the elevated oxidative stress markers in comparison of positive control group. At dose levels QILE 200 mg/kg, significant (p<0.05) protection from loss in body weight and in liver weight was found when the comparison was done with the positive control group. Histopathology revealed that QILE 200 mg/kg reduced the markers of cell necrosis. Conclusion: Present study revealed that Quercus ilex leaves have antioxidant and hepatoprotective activity due to its chemical constituents.
... While P. daemia is used as anti-helminthic, laxative, antipyretic and expectorant, and also used to treat infantile diarrhoea and malarial intermittent fevers, the latex of this plant used as a toothache cure. 11,14,15 Compared to Pergularia, Tinospora is more commonly used in various preparations of Ayurvedic medicines as various cited references suggest. ...
Article
Background: Tinospora cordifolia (Willd.) Miers, belongs to Menispermaceae, commonly known as "Guduchi"or "Amrita" and has immense importance in Ayurvedic medicine. Several studies have been carried out on pharmacology, pharmaceutical, anticancer activity and clinical trials of T. cordifolia, however not much information is available on the adulterants that are added with the genuine plant. Considerable work has been done on pharmacognosy but very few studies have been performed on the anatomy of T. cordifolia. Objectives: Comparative micro-morphoanatomical analysis of T. cordifolia and P. daemia, is important for quality control of fresh and dried samples of the root, stem and leaves. Material and Methods: The plant materials of T. cordifolia and P. daemia were collected, and identified. Fixed sample subjected to cryostat microtomy and sections were observed under light microscope. Micromorphology were studied by light as well as scanning electron microscope. Results: Anatomical studies are cheap and important tool for identification of the correct medicinal plant. Adulterant plant does not contain active principles like Berberine and Tinosporin nor have similar morpho-Anatomical characters. Because of the similar appearance and resemblance of dried adulterant plant samples, medicinally important T. cordifolia often get adulterated with other plants. SEM study revealed morpho-Anatomical differences like the abundance of starch in cortical and ray parenchyma, different types of trichomes, etc. Cryostat techniques were used for sectioning and showed the presence of xylem plates with wide multicelluar rays. Conclusion: Morphological and anatomical differences were observed in T. cordifolia and P. daemia. The given results showed significant differences therefore this study aims to help in accurate identification and avoid adulteration of a medicinally important plant.
... Carbon tetrachloride is assumed to initiate the biochemical processes leading to oxidative stress, which is the direct cause of many pathological changes in liver due to the production of free radicals. The hepatotoxic effect of CCl 4 is due to the conversion of CCl 4 to highly reactive CCl 3 molecules by cytochrome P450 enzyme present in the smooth endoplasmic reticulum of the liver and vital organs to the [23]. Highly reactive CCl 3 free radicals directly attack the endoplasmic reticulum and causes over production of SGOT, SGPT, ALP, bilirubin levels [24, 25]. ...
... [1], Alternanthera sessilis [2], Amaranthus spinosus [3], Asteraceae family plants are Achilea millefolium [4], Chrysanthemum balsamita [5], Calendula officinalis [5], Eclipta alba [6], Eclipta prostrate [7], Echinacea pallid [5], Taraxacum officinale [8]. Zingiberaceae family plants are Curcuma xanthorrhiza [9], Zingiber officinale [10], Asclepiadaceae family plants are Calotropis procera [11], Decalepis hamiltonii [12], Pergularia daemia [13], Sarcostemma brevistigma [14], Compositae family plants are Epaltes divaricata [15], Pluchea indica [16], Tridax procumbens [17]. Euphorbiaceae family plants are Emblica officinalis [18] [21], Rubiaceae family plants are Hedyotis corymbosa [22], Rubia cordifolia [23], Meliaceae family plant is Aphanamixis polystachya [24], Betulaceae family plant is Alnus japonica [25], Corylus avellana [5], Araliaceae family plant is Acathopana senticosus [26] Aegicerataceae family plant is Aegiceras corniculatum [27] Liliaceae family plant is Aloe barbadensis [28], Ranunculaceae family plant is Aquilegia vulgaris [29], Berberidaceae family plant is Berberis aristata [30], Nyctaginaceae family plant is Boerhavia diffusa [31], Chenopodiaceae family plant is Beta vulgaris [32], Theaceae family plant is Camellia oleifera [33], Apiaceae family plant is Daucus carota [34], Fumariceae family plant is Fumaria indica [35], Rutaceae family plant is Glycosmis arborea [36], Ganodermataceae family plant is Ganoderma lucidum (fungi) [37], Clusiaceae family plant is Hypericum Perforatum [38], Labiatea family plant is Hyssopus officinalis [5], Lygodiaceae family plant is Lygodium flexuosum [39], Moringaceae family is Moringa oleifera [40], Cucurbitaceae family plantis Mamordica subangulata [41], Oenotheraceae family plant is Oenothera Biennis [5], Polygalacea family plant is Polygala arvensis [42], Fabaceae family plant is Pterocarpus santalinus [43], Phyllanthaceae family plant is Phyllanthus maderaspatensis [44], Polygonaceae family plant is Rumex patientia [45], Apocynanaceae family plant is Rhazya stricta [46], Loganiaceae family plant is Strychnos potatorum [47], Gentianaceae family plant is Swertia chirata [48], Lamiaceae family is plant S. miltiorrhiza polysaccharides [49], Combretacea family plant is Termnalia arjuna [50], Aizoaceae family plant is Trianthema portulacastrum [51], Vitaceae family plant is Vitis vinifera [52], Verbenaceae family plant is Vitex trifolia [53], Scrophulariaceae family plant is Veronica officinalis [54], Solanaceae family plant is Withania frutescens [55]. ...
Article
Objectives: A lot of herbal plants and polyherbal formulations are used for the treatment of liver diseases. Methods: This present investigation was aimed to assess the hepatoprotective activity of aqueous and ethanol extract of Pongamia pinnata leaves against acetaminophen-induced liver damage in albino rats. Silymarin as a standard drug for comparing the activity. The activity was assessed by comparing the biochemical parameters in serum levels such as serum glutamate pyruvate transaminase, serum glutamate oxalate transaminase, total bilirubin, alkaline phosphatase of plant extracts treated group with acetaminophen treated animals. Results: Results showed, ethanolic extract treated group showed highly significant activity (p<0.001), whereas aqueous extract treated group has shown the significant (p<0.01) action but less compared with ethanolic extract. Plant extracts restores biochemical enzymes and brings down to normal as compared to standard drug silymarin. Conclusion: This results shows and confirms the significant protective activity against acetaminophen-induced hepatotoxicity.
... Carbon tetrachloride induces hepatotoxicity by metabolic activation therefore it selectively causes toxicity in liver cells maintaining semi normal metabolic functions. CCl4 metabolically activated by CYP450 dependent mixed oxidase in the endoplasmic reticulum to form a trichloromethyl free radical, which combined with cellular lipids and proteins in presence of oxygen to induce lipid peroxidation 7 . Highly reactive tricolour free radicals formation directly attacks the poly unsaturated fatty acids of the endoplasmic reticulum and thus cause over production of SGOT, SGPT, ALP, Total bilirubin 8 . ...
Article
Full-text available
In ancient Indian literature, it is mentioned that every plant on this earth is useful for human beings, animals and other plants. The liver is the key organ regulating homeostasis in the body. It is involved with almost all the biochemical pathways related to growth, fight against diseases, nutrient supply, energy provision and reproduction. Diospyros melanoxylon (Roxb) is native and endemic tree of India and widely found forests of central, western and northern India. This is the most common species of forests of Madhya Pradesh, Bihar, Jharkhand, Chhattisgarh, Rajasthan, Gujarat, Orissa, Andhra Pradesh and Tamil Nadu. The chemical constituents present in leaves having valuable flavones, pentacyclic triterpines. The present study was conducted to evaluate the hepatoprotective activity of ethanolic extract of leaves of Diospyros melanoxylon (Roxb) against CCl4 induced liver damage in rats. The ethanolic extract of Diospyros melanoxylonwas administered orally tothe animals with hepatotoxicity induced by CCl4. Silymarin was given as reference standard. The plant ethanolic extract was effective in protecting the liver against the injury induced by CCl4 in rats. This was evident from biochemical investigation of serum glutamate oxaloacetate transeaminase (SGOT), serum glutamic Pyruvate transeaminase (SGPT), Alkaline phosphatase (ALP) and Total Bilirubin (TB). Histopathology of CCl4 treated group rat liver showed intense centrilobular necrosis, congestion of sinusoids with small lipid globules. Whereas in ethanolic extract of leaves of Diospyros melanoxylon and Silymarin showed almost normal lobular architecture with mild centrilobular degeneration of hepatocytes without necrotic changes. It was concluded from the result that the ethanolic extract of Diospyros melanoxylon leaves possesses hepatoprotective activity against CCl4 induced hepatotoxicity in rats and it is less when compared to Silymarin as evidenced by the significant difference in biochemical parameters.
... The metabolic biotransformation of amino acid in liver by synthesis, transamination, etc., may be impaired due to the escape of both non-proteins and protein nitrogenous substances from injured cells as mediated by a raise in the serum enzyme levels of ALP, AST and ALT. The reduction in the total protein (TP) is attributed to the initial damage produced and localized in the endoplasmic reticulum which results in the loss of cytochrome P-450 enzymes leading to its functional failure with a decrease in protein synthesis and accumulation of triglycerides leading to fatty liver.[20] Both the test groups enhanced the synthesis of TP which accelerates the regeneration process and the protection of liver cells that is clearly demonstrated in Table 1. ...
Article
To investigate the hepatoprotective activity of the aqueous extract of the aerial parts of Portulaca oleracea (P. oleracea) in combination with lycopene against carbon tetrachloride induced hepatotoxicity in rats. Hepatotoxicity was induced in male Wistar rats by intraperitoneal injection of carbon tetrachloride (0.1 ml/kg b.w for 14 days). The aqueous extract of P. oleracea in combination with lycopene (50 mg/kg b.w) was administered to the experimental animals at two selected doses for 14 days. The hepatoprotective activity of the combination was evaluated by the liver function marker enzymes in the serum [aspartate transaminases (AST), alanine transaminases (ALT), alkaline phosphatase (Alk.P), total bilirubin (TB), total protein (TP) and total cholesterol (TC)], pentobarbitone induced sleeping time (PST) and histopathological studies of liver. Both the treatment groups showed hepatoprotective effect against carbon tetrachloride induced hepatotoxicity by significantly restoring the levels of serum enzymes to normal which was comparable to that of silymarin group. Besides, the results obtained from PST and histopathological results also support the study. The oral administration of P. oleracea in combination with lycopene significantly ameliorates CCl(4) hepatotoxicity in rats.
Article
Tinospora cordifolia is a plant well known for its medicinal value in Indian ayurveda and Indian traditional medicine system. However, to prove its efficiency for the clinical utilization, more experimental data will be beneficial. In the present investigation, evaluated the immunomodulatory activity of the alcoholic extracts of Tinospora cordifolia stem on various in-vivo experimental models such as neutrophil adhesion test, phagocytic index by carbon clearance test, Hemagglutinating antibody (HA) titre and delayed type hypersensitivity (DTH) responses. The evaluation of immunomodulatory potential by oral administration of alcoholic stem extracts (50, 100, 200 and 300mg/kg b.w, p.o) evoked a significant increase in percent neutrophil adhesion to nylon fibers as well as a dose dependent increased in antibody titre values, and potentiated delayed type hypersensitivity reaction induced by sheep red blood cells and significant response towards phagocytosis in carbon clearance assay (*p<0.05, *p<0.01, ***p<0.001). This may be due to concentrations of active phytochemicals present in particular plant extract. Hence, it was concluded that the plant extracts increased humoral as well as cell mediated immunity.
Article
Full-text available
Pergularia Daemai (PD) is an age-old traditional herb and is in use as folk medicine among the villagers even today. It had been screened for its bioactive compounds and various pharmacological activities. But its anti-inflammatory activity is not explored fully. In the present venture, we evaluated the anti-inflammatory effect of ethanolic extract of PD by in vitro method by using membrane stabilization test and protein denaturation test. Membrane stabilization test was done by using human red blood cells (HRBCs). Protein denaturation test was done by using bovine serum albumin (BSA). The results revealed that PD extract was capable of rendering membrane stabilization by inhibiting the hypotonically-induced hemolysis of HRBCs in dose-dependent manner (50, 100, 200, 300, 400, 500 and 1000 μg/mL). In lesser concentration (50 μg/mL), the % inhibition of hemolysis was less (26.80%) and in higher concentration (1000 μg/mL), the % inhibition of hemolysis was more (76.30%), which was comparable with that of standard antiinflammatory drug viz. diclofenac sodium (200 μg/mL – 80.60%). The PD extract was also capable of inhibiting BSA denaturation in dose-dependent manner (50 μg/mL – 20.40%, 1000 μg/mL – 83.60%) which was comparable to that of diclofenac sodium (200 μg/mL – 86.60%). This finding confirms the potentiality of PD extract as an anti-inflammatory agent and justifies the recommendation of PD extract for the treatment of painful inflammatory conditions.
ResearchGate has not been able to resolve any references for this publication.