Regulation of integrin α2, α5, β1, mRNA expression on osteoblasts membrane by bFGF

Department of Prosthodontics, West China Stomatological Hospital, Sichuan University, Chengdu 610041, China.
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition 12/2008; 39(6):909-11.
Source: PubMed


To observe the regulation of bFGF on mRNA expression of integrin subunits alpha2, alpha5, beta1, in osteoblasts.
Osteoblasts were pre-cultured for 24 h with F12 medium containing bFGF with different concentration and planted on the sand blasting titanium disks. Three days after planting, osteoblasts were harvested. The mRNA expression of integrin subunit alpha2, alpha5, beta1, were examined by immunofluorescent quantitative real-time polymerase chain reaction (RT-PCR).
The mRNA expression of integrin beta1 is most abundant and the mRNA expressions of integrin alpha2, as are relatively lower than integrin beta1. In the study, the mRNA expression levels of integrin alpha2, alpha5 and beta1 were up-regulated after bFGF stimulation. However, the effective concentration range of bFGF to the three subunits was different.
bFGF could up-regulate the mRNA expression of integrin subunits in osteoblasts.

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