Melatonin present in beer contributes to increase the levels of melatonin and antioxidant capacity of the human serum

Department Medical Biochemistry and Molecular Biology, University of Seville Medical School, Avda. Sánchez Pizjuán 4, 41009 Seville, Spain.
Clinical nutrition (Edinburgh, Scotland) (Impact Factor: 4.48). 02/2009; 28(2):188-91. DOI: 10.1016/j.clnu.2009.02.001
Source: PubMed


Melatonin is a molecule with antioxidative properties including direct free radical scavenging and indirect stimulatory actions on a variety of antioxidative enzymes which further promote its ability to reduce the toxicity of radicals and their associated reactants. Beer is an integral element of the diet of numerous people and is rich in antioxidants. We analyzed if melatonin is present in beer and if so, at what concentration. It further determines whether the moderate consumption of beer has an effect on the total antioxidant status (TAS) of human serum.
We analyzed 18 brands of beer with different percentage of alcohol content in order to determine the concentration of melatonin. Serum samples were collected from 7 healthy volunteers. These samples were used to measure melatonin and TAS on basal conditions and after drinking beer.
Showed that all the beer analyzed did indeed contain melatonin and the more they have got, the greater was its degree of alcohol. Both melatonin and TAS in human serum increased after drinking beer.
Melatonin present in the beer does contribute to the total antioxidative capability of human serum and moderate beer consumption can protect organism from overall oxidative stress.

Download full-text


Available from: Maria D Maldonado, Mar 23, 2015
    • "ACCEPTED MANUSCRIPT 5 after consumption of the beer and melatonin was assayed by ELISA. (Maldonado, et al., 2009). While the actual average increase in plasma melatonin was not reported, based upon data presented infigure 1 of the paper, it can be estimated to be approximately 2.1 pg/ml. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Melatonin has been proposed as a potent anti-oxidant and its presence in many plants and foods has been suggested to be beneficial for health. Indeed, the concentrations of melatonin in blood, and the melatonin metabolite 6 sulphatoxymelatonin in urine, have been found to increase significantly after ingestion of melatonin rich foods. In this review the studies have been critically evaluated in light of the reported plant melatonin concentrations and our knowledge of pharmacokinetics of orally administered pure melatonin. In the case of studies involving measurement of plasma melatonin following ingestion of beer or fruits the reported increases in melatonin are not consistent with the amount of melatonin ingested. Similarly the amount of melatonin metabolite excreted following ingestion of melatonin rich foods greatly exceed the amount ingested. It is concluded that studies reporting the appearance of melatonin in blood and its metabolites in urine following ingestion of melatonin rich foods are flawed. While there may be health benefits for certain foods it is difficult to accept that they are due to their low melatonin content.
    No preview · Article · May 2015 · Critical reviews in food science and nutrition
  • Source
    • "Regarding the presence or production of melatonin in yeast, the pioneer study of Sprenger et al. [28] related the presence of S. cerevisiae and the production of melatonin. Later, some reports detected melatonin in wines [44–46] and beer [47]. Recent studies also describe melatonin in grapes and other tissues of the vine, which could indicate that the origin was the substrate [23, 48, 49]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Metabolites resulting from nitrogen metabolism in yeast are currently found in some fermented beverages such as wine and beer. Their study has recently attracted the attention of researchers. Some metabolites derived from aromatic amino acids are bioactive compounds that can behave as hormones or even mimic their role in humans and may also act as regulators in yeast. Although the metabolic pathways for their formation are well known, the physiological significance is still far from being understood. The understanding of this relevance will be a key element in managing the production of these compounds under controlled conditions, to offer fermented food with specific enrichment in these compounds or even to use the yeast as nutritional complements.
    Full-text · Article · May 2014 · BioMed Research International
  • Source
    • "Differences between the melatonin content found by ELISA and HPLC methods are not unexpected. Although immunoassay technique (ELISA) has been widely used for identification and quantification of melatonin in plants [19,21-23], and some studies have shown good correlation with HPLC [24-26], ELISA does have some cross-reactivity with structurally similar compounds, potentially causing an overestimation of melatonin content. In this study, ELISA was used to confirm the presence of the melatonin in samples in the absence of mass spectra (MS) data. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Melatonin content was screened in leaves of seven edible herbs used as sleeping aids in Thai traditional medicine. These plants are Piper nigrum L, Sesbania glandiflora (L.) Desv., Sesbania sesban (L.) Merr., Senna tora (L.) Roxb., Moringa oleifera Lam., Momordica charantia L. and Baccaurea ramiflora Lour. Dried leaves were extracted by sonication in methanol for six hours at room temperature, and then melatonin was purified by C18 solid phase extraction (SPE). Melatonin was then quantified by a validated RP-C18 HPLC method with fluorescent detection. Melatonin contents in extracts of B. ramiflora, S. glandiflora, M. charantia, S. tora and S. sesban were 43.2, 26.3, 21.4, 10.5 and 8.7 ng/g of dry sample weight, respectively. The highest melatonin content was from P. nigrum extract (1092.7 ng/g of dry sample weight). Melatonin was not detected in the extract of M. oleifera. Melatonin identification was confirmed by ELISA. Melatonin was found in six of the seven herbs in the traditional Thai sleeping recipe. One of these, P. nigrum, exhibited an encouragingly high amount of melatonin.
    Full-text · Article · Jan 2014 · DARU-JOURNAL OF FACULTY OF PHARMACY
Show more