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Melatonin present in beer contributes to increase the levels of melatonin and antioxidant capacity of the human serum

Department Medical Biochemistry and Molecular Biology, University of Seville Medical School, Avda. Sánchez Pizjuán 4, 41009 Seville, Spain.
Clinical nutrition (Edinburgh, Scotland) (Impact Factor: 4.48). 02/2009; 28(2):188-91. DOI: 10.1016/j.clnu.2009.02.001
Source: PubMed

ABSTRACT

Melatonin is a molecule with antioxidative properties including direct free radical scavenging and indirect stimulatory actions on a variety of antioxidative enzymes which further promote its ability to reduce the toxicity of radicals and their associated reactants. Beer is an integral element of the diet of numerous people and is rich in antioxidants. We analyzed if melatonin is present in beer and if so, at what concentration. It further determines whether the moderate consumption of beer has an effect on the total antioxidant status (TAS) of human serum.
We analyzed 18 brands of beer with different percentage of alcohol content in order to determine the concentration of melatonin. Serum samples were collected from 7 healthy volunteers. These samples were used to measure melatonin and TAS on basal conditions and after drinking beer.
Showed that all the beer analyzed did indeed contain melatonin and the more they have got, the greater was its degree of alcohol. Both melatonin and TAS in human serum increased after drinking beer.
Melatonin present in the beer does contribute to the total antioxidative capability of human serum and moderate beer consumption can protect organism from overall oxidative stress.

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Available from: Maria D Maldonado, Mar 23, 2015
    • "ACCEPTED MANUSCRIPT 5 after consumption of the beer and melatonin was assayed by ELISA. (Maldonado, et al., 2009). While the actual average increase in plasma melatonin was not reported, based upon data presented infigure 1 of the paper, it can be estimated to be approximately 2.1 pg/ml. "
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    • "Regarding the presence or production of melatonin in yeast, the pioneer study of Sprenger et al. [28] related the presence of S. cerevisiae and the production of melatonin. Later, some reports detected melatonin in wines [44–46] and beer [47]. Recent studies also describe melatonin in grapes and other tissues of the vine, which could indicate that the origin was the substrate [23, 48, 49]. "
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    • "Differences between the melatonin content found by ELISA and HPLC methods are not unexpected. Although immunoassay technique (ELISA) has been widely used for identification and quantification of melatonin in plants [19,21-23], and some studies have shown good correlation with HPLC [24-26], ELISA does have some cross-reactivity with structurally similar compounds, potentially causing an overestimation of melatonin content. In this study, ELISA was used to confirm the presence of the melatonin in samples in the absence of mass spectra (MS) data. "
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