Frequency of distribution of inflammatory cytokines IL-1, IL-6 and TNF-α gene polymorphism in patients with obstructive sleep apnea

Department of Laboratory Medicine and Clinical Immunology of Developmental Age, Warsaw Medical University, Warsaw, Poland.
Journal of physiology and pharmacology: an official journal of the Polish Physiological Society (Impact Factor: 2.39). 01/2009; 59 Suppl 6:607-14.
Source: PubMed


Obesity is one of the most commonly identified factors for the obstructive sleep apnea syndrome (OSAS). Adipose tissue is the source of many cytokines, among them there are IL-6, IL-1, and TNF-alpha. The level of inflammatory cytokines increases in people with OSAS and obesity. The aim of this study was to evaluate the distribution of genotypes in inflammatory cytokine genes in people with obesity-related OSAS. The examined group consisted of 102 person with obesity related-OSAS and 77 normal weight person without OSAS. Genotyping of DNA sequence variation was carried out by restriction enzyme (IL-1: Taq I, IL-6: Lwe I, TNF-alpha: Nco I) analysis of PCR amplified DNA. The study revealed a significant correlation between polymorphism located in the promoter region of inflammatory cytokine genes and obesity-related OSAS.

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Available from: Urszula Demkow
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    • "The other studies were from western countries: one from the United Kingdom [16], one from Poland [15], and one from Greece [10]. Regarding ethnicity, four studies concerned patients of Asian origin [11], [12], [13], [14], three concerned patients of Caucasian origin [10], [15], [16]. For the determination of the genetic polymorphism, validated genotyping methods were used in all studies. "
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    ABSTRACT: Background: Several studies have reported that the tumor necrosis factor-alpha (TNF-alpha) -308G/A polymorphism is associated with susceptibility to obstructive sleep apnea-hypopnea syndrome (OSAHS). However, these results are controversial and conflicting. Objective: To evaluate the association between TNF-alpha-308G/A and OSAHS risk by meta-analysis. Methods: Electronic databases, including PubMed, Embase, China National Knowledge Infrastructure (CNKI), Wanfang, and Weipu, were searched to identify relevant studies. Data were extracted from the included studies. A model-free approach using odds ratio (OR), generalized odds ratio (ORG) and 95% confidence interval (CI) of the allele contrast to assess the association between the -308G/A polymorphism and OSAHS risk. Cumulative and recursive cumulative meta-analyses (CMA) were also carried out to investigate the trend and stability of effect sizes as evidence accumulated. Results: Seven studies including 1369 OSAHS patients and 1064 controls were identified in this meta-analysis. Significant associations were derived from the variants of the allele contrast [(OR, 1.78; 95% CI, 1.45-2.18) or (ORG, 2.01; 95% CI, 1.27-3.19). CMA showed a trend of an association. Recursive CMA indicated that more evidence is needed to conclude on the status of significance. No significant publication bias was found. Conclusions: Our meta-analysis suggested that the TNF-alpha-308G/A polymorphism contribute to the risk of OSAHS. Further studies with larger sample should be performed to confirm our findings.
    Full-text · Article · Sep 2014 · PLoS ONE
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    • "There are many interesting reports on this subject that have been accurately evaluated in a recent review 8. There are also some studies that demonstrate the increase of inflammatory factors in subjects with OSAS: among these, C-reactive protein and cytokines, which are responsible for systemic atherosclerosis and probably have a role in the appearance of neoplasms 9 10. There is also evidence of an increase of atherogenic dyslipidaemia in subjects with OSAS 11. "
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    ABSTRACT: This draft of the Official Round Table held during the 99th SIO National Congress is an updated review on the diagnostic tools, the importance of polysomnographic recording and a critical analysis of the surgical techniques in obstructive sleep apnoea syndrome (OSAS). The review and analysis of available publications is the premise along with a specific analysis of the relationship between OSAS and metabolic and vascular disorders. In addition, the most recent investigations on sleep disorders and altered glucose metabolism are summarised and discussed together with the results of a study by the authors involving a fairly large number of patients with OSAS and diabetes.
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    • "However, only a limited number of studies have evaluated the association between cytokine gene polymorphisms and sleep disturbance. For example, in one study that examined single nucleotide polymorphisms (SNPs) in interleukin 6 (IL6), IL1, and tumor necrosis factor alpha (TNFA) in patients newly diagnosed with obstructive sleep apnea syndrome (OSAS) (Popko et al., 2008), the only cytokine gene that was associated with OSAS was a polymorphism located in the promoter region of IL6 (rs1800795). In addition, this association was found only in male patients with OSAS compared to unaffected males. "
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