Article

Genomic Epidemiology of a Dengue Virus Epidemic in Urban Singapore

Novartis Institute for Tropical Diseases, Chromos, Singapore.
Journal of Virology (Impact Factor: 4.44). 03/2009; 83(9):4163-73. DOI: 10.1128/JVI.02445-08
Source: PubMed

ABSTRACT

Dengue is one of the most important emerging diseases of humans, with no preventative vaccines or antiviral cures available
at present. Although one-third of the world's population live at risk of infection, little is known about the pattern and
dynamics of dengue virus (DENV) within outbreak situations. By exploiting genomic data from an intensively studied major outbreak,
we are able to describe the molecular epidemiology of DENV at a uniquely fine-scaled temporal and spatial resolution. Two
DENV serotypes (DENV-1 and DENV-3), and multiple component genotypes, spread concurrently and with similar epidemiological
and evolutionary profiles during the initial outbreak phase of a major dengue epidemic that took place in Singapore during
2005. Although DENV-1 and DENV-3 differed in viremia and clinical outcome, there was no evidence for adaptive evolution before,
during, or after the outbreak, indicating that ecological or immunological rather than virological factors were the key determinants
of epidemic dynamics.

  • Source
    • "The outbreaks of dengue have been proposed to have begun in Asia, where the greatest number of dengue cases is currently found. Almost all countries in Southeastern Asia (e.g., Indonesia, Cambodia, Laos, Myanmar, Malaysia, Philippines, Thailand, and Vietnam) have documented epidemic dengue outbreaks (Dorji et al., 2009; Gubler, 1998; Holmes et al., 2009; Jarman et al., 2008; Schreiber et al., 2009; Teoh et al., 2010). DENV is a member of the Flavivirus genus belonging to the Flaviviridae family, with a single stranded, non-segmented, positivesense RNA genome of approximately 10.7 kb that encodes a single open reading frame for three structural proteins (C, capsid; prM/M, precursor of membrane; and E, envelope) and seven non-structural proteins (NS1, NS2a, NS2b, NS3, NS4a, NS4b, and NS5) (Chambers et al., 1990). "
    [Show abstract] [Hide abstract]
    ABSTRACT: Since 2000, sporadic imported cases of dengue fever were documented almost every year in Yunnan Province, China. Unexpectedly, a large-scale outbreak of dengue virus (DENV) infection occurred fromAugust to December 2013,with 1538 documented cases. In the current study, 81 dengue-positive patient sampleswere collected from Xishuangbanna, the southernmost prefecture of the Yunnan province, and 23 from Dehong, the westernmost prefecture of the Yunnan province. The full-length envelope genes were amplified and sequenced. Phylogenetic analysis revealed that nine strains (39.1%) and 14 strains (60.9%) from the Dehong prefecture were classified as genotype I of DENV-1 and Asian I genotype of DENV-2, respectively. All strains from Xishuangbanna were identified as genotype II of DENV-3. Bayesian coalescent analysis indicates that the outbreak originated from bordering southeastern Asian countries. These three epidemic genotypes were predicted to originate in Thailand and then migrate into Yunnan through different routes.
    Full-text · Article · Oct 2016 · Infection Genetics and Evolution
  • Source
    • "The outbreaks of dengue have been proposed to have begun in Asia, where the greatest number of dengue cases is currently found. Almost all countries in Southeastern Asia (e.g., Indonesia, Cambodia, Laos, Myanmar, Malaysia, Philippines, Thailand, and Vietnam) have documented epidemic dengue outbreaks (Dorji et al., 2009; Gubler, 1998; Holmes et al., 2009; Jarman et al., 2008; Schreiber et al., 2009; Teoh et al., 2010). DENV is a member of the Flavivirus genus belonging to the Flaviviridae family, with a single stranded, non-segmented, positivesense RNA genome of approximately 10.7 kb that encodes a single open reading frame for three structural proteins (C, capsid; prM/M, precursor of membrane; and E, envelope) and seven non-structural proteins (NS1, NS2a, NS2b, NS3, NS4a, NS4b, and NS5) (Chambers et al., 1990). "
    [Show abstract] [Hide abstract]
    ABSTRACT: Since 2000, sporadic imported cases of dengue fever were documented almost every year in Yunnan Province, China. Unexpectedly, a large-scale outbreak of dengue virus (DENV) infection occurred from August to December 2013, with 1538 documented cases. In the current study, 81 dengue-positive patient samples were collected from Xishuangbanna, the southernmost prefecture of the Yunnan province, and 23 from Dehong, the westernmost prefecture of the Yunnan province. The full-length envelope genes were amplified and sequenced. Phylogenetic analysis revealed that nine strains (39.1%) and 14 strains (60.9%) from the Dehong prefecture were classified as genotype I of DENV-1 and Asian I genotype of DENV-2, respectively. All strains from Xishuangbanna were identified as genotype II of DENV-3. Bayesian coalescent analysis indicates that the outbreak originated from bordering southeastern Asian countries. These three epidemic genotypes were predicted to originate in Thailand and then migrate into Yunnan through different routes.
    Full-text · Article · Oct 2015 · Infection, genetics and evolution: journal of molecular epidemiology and evolutionary genetics in infectious diseases
  • Source
    • "008 ) and Surabaya ( Yamanaka et al . , 2011 ) . The clustering of all Genotype I samples together with viruses from China , Thailand , and Singapore may suggest that the Genotype I isolates were originated from China , migrated to Thailand and passed Singapore before entering Makassar ( Fig . 1 ) . Genotype IV was predominant in Jakarta in 2004 ( Schreiber et al . , 2009 ) and was also present in Makassar in smaller numbers ."

    Full-text · Article · Mar 2015 · Infection Genetics and Evolution
Show more