Our previous studies demonstrated that precoating of filter fibres with biologically active tea tree oil (TTO) enhances physical collection efficiency of conventional HVAC filters and provides cost effective and rapid inactivation of captured bacterial and fungal particles on the filter surface. The main aim of this study was to investigate the antiviral activity of two natural disinfectants, i.e. TTO and eucalyptus oil (EUO) against the influenza virus captured onto the filter surface. It was found that both tested oils possess strong antiviral properties when used as fibre coating materials, capable of inactivating captured microorganisms within 5–10 minutes of contact on the fibre surface. The antiviral activity of TTO was also successfully challenged in aerosol form by mixing viable airborne viral particles with oil droplets in the rotational aerosol chamber. The results look very promising for further development of virus inactivating procedures and technologies for air quality applications.