DNA methylation status is inversely correlated with green tea intake and physical activity in gastric cancer patients. Int J Cancer

Department of Molecular Oncology, Tokyo Medical and Dental University, Tokyo, Japan.
International Journal of Cancer (Impact Factor: 5.09). 06/2009; 124(11):2677-82. DOI: 10.1002/ijc.24231
Source: PubMed


Epigenetic silencing of genes by aberrant DNA methylation is recognized as a crucial component of the mechanism underlying tumorigenesis. However, the relationship between DNA methylation and the past lifestyle in cancer patients remains largely unknown. We examined the methylation statuses of 6 tumor-related genes, CDX2 (homeobox transcription factor), BMP-2 (bone morphogenetic protein 2), p16 (INK4A), CACNA2D3 (calcium channel-related), GATA-5 (transcription factor) and ER (estrogen receptor), in 106 primary gastric carcinomas by methylation-specific PCR and compared them with the past lifestyles of the patients. The methylation frequencies of the genes were 23.6, 21.7, 9.4, 32.4, 40.8 and 59.1%, respectively. Significant association was found between a decreased intake of green tea and methylation of CDX2 and BMP-2. More physical activity was correlated with a lower methylation frequency of CACNA2D3. Of these 6 genes, the methylation statuses of CDX2, BMP-2 and p16 revealed a significant interrelationship and those of CACNA2D3, GATA-5 and ER did likewise. Thus, some epidemiological factors, such as green tea intake, could be important as to determination of the methylation statuses of selected genes and may influence the development of cancer, including that of the stomach.

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    • "Regardless of the health status of the studied population , observational studies consistently show a weak correlation between physical activity and global or gene-specific DNA methylation. However, in most of these studies, physical activity was assessed via questionnaires at the time the study was conducted (Slattery et al. 2007, Pirola et al. 2012, Luttropp et al. 2013), before cancer onset (Yuasa et al. 2009), for the past week (Lott et al. 2012), for the past year (Coyle et al. 2007, Morabia et al. 2012, Zhang et al. 2012, White et al. 2013), the past 5 years (Coyle et al. 2007), the past 10 or 20 years (Slattery et al. 2007) and over a lifetime (Coyle et al. 2007). Therefore, the possibility of recall errors from the participants may have generated an important bias (Matthews 2002, Lissner et al. 2004). "
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    • "Assessment of a large group of unrelated gastric cancer and normal tissue found that the CACNA2D3 CpG island was methylated in 30% of primary tumours, and 5.3% of normal tissue (Wanajo et al, 2008). A similar rate of methylation was reported in an independent cohort of gastric cancers (Yuasa et al, 2009). A significantly reduced survival time was found in tumours with methylation as compared with those with unmethylated CACNA2D3. "
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    • "5.1. Clinical data As summarized in Table 3 and 24 studies to date have investigated the effects of structured exercise training on changes in circulating concentrations of host-related factors in persons with cancer (Allgayer et al., 2004, 2008a; Evans et al., 2009; Fairey et al., 2003, 2005a,b; Galvao et al., 2010; George et al., 2010; Irwin et al., 2006, 2007, 2005, 2009; Janelsins et al., 2011; Jones et al., 2012b; Ligibel et al., 2008; Na et al., 2000; Payne et al., 2008; Pierce et al., 2009; Schmitz et al., 2005; Segal et al., 2003, 2009; Tosti et al., 2011; Yuasa et al., 2009; Zeng et al., 2011). "
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