The present study used data from two archival samples to test the hypothesis, derived from Worthy, M. (1999), Eye colour: a key to human and animal behaviour. Lincoln, Nebraska: to Exel (originally published 1974) that light-eyed individuals would be more likely than dark-eyed individuals to abuse alcohol. Sample 1 consisted of 10,860 Caucasian male prison inmates, and Sample 2 consisted of 1862 Caucasian women respondents in a national survey. In both samples, individuals with light eyes had consumed significantly more alcohol than individuals with dark eyes. These results are consistent with previous findings that dark-eyed people exhibit more physiological arousal and more sensitivity to some medications than light-eyed people. The results may indicate that greater sensitivity to alcohol in dark-eyed individuals prevents them from drinking the large quantities of alcohol needed for development of physical dependence. Alternatively, greater behavioral inhibition may motivate light-eyed individuals to engage in alcohol consumption to achieve harm avoidance.