Detection of Illicium anisatum as Adulterant of Illicium verum

National Center for Natural Products Research and Research Institute of Pharmaceutical Sciences, Department of Pharmacognosy, School of Pharmacy, University of Mississippi, University, MS 38677, USA.
Planta Medica (Impact Factor: 2.15). 02/2009; 75(4):392-5. DOI: 10.1055/s-0028-1112219
Source: PubMed


Chinese Star anise, Illicium verum Hook, is a well known spice in many cultures and has also been used to treat infant colic. Recent publications report that Chinese Star anise might be adulterated with the toxic Japanese Star anise, Illicium anisatum L. We have developed a molecular method that helps with the detection of I. anisatum as adulterant of I. verum. We PCR-amplified the internal transcribed spacer (ITS) region and analyzed it with the endonucleases PSTI and BFMI. Based on fragment length polymorphisms (RFLP), we were able to detect and distinguish between I. anisatum and other Illicium species in powdered samples.

Download full-text


Available from: Natascha Techen
  • Source
    • ". Fluorescence microscopy, thermal desorption–gas chromatography–mass spectrometry, thin layer chromatography (TLC), high performance liquid chromatography coupled to mass spectrometry (HPLC-MS/MS), DNA sequencing and restriction fragment length polymorphism (RFLP) methods have all been proposed with varying levels of success [10] [14] [16] [18] "
    [Show abstract] [Hide abstract]
    ABSTRACT: Illicium verum (Chinese star anise) dried fruit is popularly used as a remedy to treat infant colic. However, instances of life-threatening adverse events in infants have been recorded after use, in some cases due to substitution and/or adulteration of I. verum with Illicium anisatum (Japanese star anise), which is toxic. It is evident that rapid and efficient quality control methods are of utmost importance to prevent re-occurrence of such dire consequences. The potential of short wave infrared (SWIR) hyperspectral imaging and image analysis as a rapid quality control method to distinguish between I. anisatum and I. verum whole dried fruit was investigated. Images were acquired using a sisuChema SWIR hyperspectral pushbroom imaging system with a spectral range of 920-2514nm. Principal component analysis (PCA) was applied to the images to reduce the high dimensionality of the data, remove unwanted background and to visualise the data. A classification model with 4 principal components and an R(2)X_cum of 0.84 and R(2)Y_cum of 0.81 was developed for the 2 species using partial least squares discriminant analysis (PLS-DA). The model was subsequently used to accurately predict the identity of I. anisatum (98.42%) and I. verum (97.85%) introduced into the model as an external dataset. The results show that SWIR hyperspectral imaging is an objective and non-destructive quality control method that can be successfully used to identify whole dried fruit of I. anisatum and I. verum. In addition, this method has the potential to detect I. anisatum whole dried fruits within large batches of I. verum through upscaling to a conveyor belt system.
    Full-text · Article · Dec 2012 · Journal of pharmaceutical and biomedical analysis
  • [Show abstract] [Hide abstract]
    ABSTRACT: In this study we designed specific primer pairs for identification of toxic plants such as subgenus Aconitum, genus Ricinus and genus Illicium in internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA. Allied species of target plants and foods were not detected, but each primer pairs give a PCR product in the presence of target plant.This PCR method is useful for identification of toxic plants and can detect subgenus Aconitum, genus Illicium and genus Ricinus with template DNA of 1ng, 100pg and 10pg, respectively.
    No preview · Article · Dec 2009 · Forensic Science International Genetics Supplement Series
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Whether they are being taken as dietary supplements by the general public or being evaluated in a clinical study, the authenticity of botanical products is a matter of paramount concern. Botanical specimens and the dietary supplements derived from them can vary in quality and in chemical constituent profiles because of a number of factors. Subtle variations in botanical specimens are known to have profound effects on the quality, efficacy, and safety of botanical dietary supplements and can potentially alter the results of clinical studies that rely on these materials. A complete array of authentication and evaluation tools can be utilized to provide a well-rounded scientific approach to the authentication of botanical products. It is vital that the authenticity of botanical supplements be established using appropriate analysis tools regardless of whether the end products are being considered for evaluation in clinical studies or are being developed for the consumer market.
    Preview · Article · Feb 2010 · Clinical Pharmacology &#38 Therapeutics
Show more