Biochemical, Hematological, and Electrocardiographic Changes in Buffaloes Naturally Infected with Theileria annulata

Department of Clinical Science, Faculty of Veterinary Medicine, Islamic Azad University Tabriz Branch, Tabriz, Iran.
The Korean Journal of Parasitology (Impact Factor: 1.15). 01/2009; 46(4):223-7. DOI: 10.3347/kjp.2008.46.4.223
Source: PubMed


Changes in selected blood and serum components and electrocardiography (ECG) were investigated in 20 adults (13 females and 7 males) of water buffaloes suffering from severe theileriosis. The age of all animals used in this study ranged 1.5-5 yr. Theileriosis was diagnosed by observation of parasites in the peripheral blood and the presence of schizonts in lymphocytes that were provided from swollen lymph nodes. Statistically significant decreases were observed in the means of RBC, WBC, and packed cell volume (PCV) in blood of infected animals. The means levels of sodium, calcium, phosphorus, and potassium of infected animals were lower than healthy animals, but only the decrease of potassium was significant. The mean serum activities of aspartate transferase and alanine aminotransferase were significantly higher than in uninfected animals. Three cases had atrial premature beat, 2 cases had sinus tachycardia, 2 had sinus arrhythmia, and 1 had first degree of atrioventricular block in ECG. The present study showed that T. annulata infection in cattle is associated with hematological and biochemical, and ECG changes.

Download full-text


Available from: Gholamali Moghaddam, Sep 26, 2015
  • Source
    • "Seven species of Theileria are known to infect cattle; of these, T. annulata is of major importance (Hasanpour et al., 2008). It is difficult to differentiate Theileria species solely on the basis of the morphology of the piroplasm and schizont stages and confusion may arise if mixed infections occur (Dumanli et al., 2005). "
    [Show abstract] [Hide abstract]
    ABSTRACT: The present study was carried out to determine the prevalence of Theileria annulata in large ruminants from two districts, Peshawar and Kohat, in Khyber Pukhtoon Khwa (Pakistan). Blood samples were collected from 95 cattle. Data on the characteristics of animals and herds were collected through questionnaires. No significant risk factors were found associated with the spread of tropical theileriosis in the study area. Two different parasite detection techniques, PCR amplification and screening of Giemsa stained slides, were compared and it was found that PCR amplification is a more sensitive tool (33.7% parasite detection), as compared to smear scanning (5.2% parasite detection) for the detection of Theileria annulata. 32 out of 95 animals, from both districts, produced the 721-bp fragment specific for Theileria annulata.
    Full-text · Article · Feb 2012 · Parasite
  • Source
    • "This overall increase in the TLC serves as a defense phenomena which is a characteristic of helminth infection (Hasanpour et al. 2008). A persual of existing literature revealed that cysticercosis may result in eosinophilia in peripheral blood (Vanajakshi et al. 1984; Sinha 2000; Hasanpour et al. 2008). In our study peaks of eosinophilia occurred at 10, 30, and 60 days pi (Fig. 2). "
    [Show abstract] [Hide abstract]
    ABSTRACT: The present study pertains to the haematological examination of the rats experimentally infected with cysticercosis which revealed significant alterations in several parameters. Haemoglobin concentration was observed to be significantly lower in later stages of cysticercosis with respect to the control rats whereby 36% decline was observed at 60 days post infection (pi). These changes in haemoglobin were more or less parallel to those recorded for RBC counts with 18% reduction at 60 days pi. A significant rise was noticed in the erythrocyte sedimentation rates throughout the course of infection with a maximum increase (42%) at 60 days pi whereas a decline in packed cell volume as the cyst development was also evident. Leucocytosis was observed at 10, 30 and 60 days post infection with 2.6, 2.5 and 1.5 fold increase, respectively. Moreover, haematological indices were prominently altered by cysticercosis indicating macrocytic hypochromia in advance stages of the cyst development.
    Preview · Article · Oct 2011 · Journal of parasitic diseases
  • Source
    • "Later, the merozoites invade red blood cells (Forsyth et al. 1999; Campbell and Spooner 1999; Graham et al. 2001). Serum biochemical analysis of cattle naturally and experimentally infected with Theileria sp. was carried out by various researchers (Watanabe et al. 1998; Sandhu et al. 1998; Omer et al. 2003; Col and Ulsu 2007; Hasanpour et al. 2008), and the results of these studies produced some different opinions. Although the mechanism of the anemia in theileriosis has not been fully elucidated, erythrophagocytosis due to immune-mediated "
    [Show abstract] [Hide abstract]
    ABSTRACT: Analyses for alanine aminotransferase, aspartate aminotransferase (AST), alkaline phosphatase (ALP) activities, and iron concentration were carried out in infected and uninfected cattle in order to determine the degree of hepatic damage and blood iron status caused by Theileria annulata and Babesia bigemina. Blood samples from 30 infected and 20 uninfected cattle with T. annulata and also from 40 infected and 20 uninfected cattle with B. bigemina were taken. The biochemical analyses revealed increased serum AST and ALP activities in infected cattle with T. annulata and B. bigemina compared with uninfected cattle (P < 0.05). Significant decreases in iron concentration were observed in cattle infected with T. annulata and B. bigemina when compared with uninfected cattle (P < 0.05) although the level of serum iron concentration in infected cattle was within the normal reference values for cattle. It was concluded that iron deficiency anemia is not an important factor in T. annulata- and even in B. bigemina-infected cattle with severe intravascular hemolysis. The increased activities of serum enzymes indicated the hepatic injuries associated with infection with T. annulata and B. bigemina. KeywordsCattle– Theileria annulata – Babesia bigemina –Iron–Serum enzymes
    Full-text · Article · Oct 2011 · Comparative Clinical Pathology
Show more