Hiscox JD, Israelstam GF A method for the extraction of chlorophyll from leaf tissue without maceration. Can J Bot

Canadian Journal of Botany (Impact Factor: 1.4). 01/2011; 57(12):1332-1334. DOI: 10.1139/b79-163


A simple, rapid method requiring few manipulations for the extraction of chlorophylls from fragmented leaf tissue of angiosperms and gymnosperms is compared with the widely used acetone method. Unlike the acetone method where grinding and subsequent centrifugation are essential, this method makes use of incubation at 65 °C of leaf tissue immersed in dimethyl sulphoxide. The new method was found to be as efficient as acetone for chlorophyll extraction and superior in terms of chlorophyll stability.

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    • "The green leaves were taken for chlorophyll estimation according to the method of Hiscox and Israelstam ( 1979 ) . One hundred mg of tomato leaf pieces were kept in a vial containing 7ml of a chemical dimethyl sulfoxide ( DMSO ) and the chlorophyll content was extracted into the fluid by incubating for 60min . "

    Full-text · Article · Dec 2015
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    • "The photosynthetic pigments were extracted from leaves of tomato plants in dimethyl sulfoxide (DMSO) as described by Hiscox and Israelstam (1979 "
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    ABSTRACT: The aim of present study was to examine the effect of arbuscular mycorrhizal fungi (AMF) on the growth, lipid peroxidation, antioxidant enzyme activity and some key physio-biochemical attributes in cowpea (Vigna unguiculata [L.] Walp.) subjected to salt stress. Salt stress (200 mM NaCl) reduced growth, biomass, relative water content and chlorophyll pigment content in cowpea leaves. AMF ameliorated the negative impact of salinity on the growth parameters studied. The activity of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), peroxidase (POD) and glutathione reductase (GR) enhanced under salt stress and AMF inoculation further enhanced their activity, thus strengthening the plant’s defense system. Proline content increased in salt stressed plants as well as AMF-inoculated plants providing efficient protection against salt stress. Besides this AMF also increased uptake of mineral elements which have direct impact on the osmoregulation of the plants. The present study shows that AMF possesses the potential to enhance salt tolerance of cowpea.
    Full-text · Article · Oct 2015 · Legume Research
    • "CRI stage. Photosynthetic pigments like chlorophyll a and b were estimated according to the method of Hiscox and Israel and Tam (1979 using dimethyl sulphoxide (DMSO) chemical under laboratory conditions. Chlorophyll concentration index (CCI) was recorded using Apogee's CCM-200 instrument. "
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    ABSTRACT: Abstract A field experiment was conducted for three cultivars of wheat i.e. PBW 343, PBW550 and DBW17 with five nitrogen levels i.e. 0, 75, 125, 175 and 225 kg N/ha in split plot design. Growth parameters like LAI, chlorophyll content, nitrogen, chlorophyll concentration index (CCI) and plant height were recorded. Spectral indices such as Normalized difference Vegetation Index (NDVI), Ratio Vegetation Index (RVI), Moisture Stress Index (MSI), Green Index (GI), Leaf Chlorophyll Index (LCI), Plant Senescence Reflectance Index (PSRI), were computed using the multiband spectral data. Among the different phenological phases of crop growth, best correlations were observed between different growth parameters and vegetation indices at booting stage of wheat. The spectral indices selected for the study recorded at booting stage were correlated better with all the crop parameters having ‘R2’ values varied between 0.68 and 0.83. All the spectral indices were having good correlation (R2) ranging between 0.58 and 0.82 for grain yield and 0.63–0.80 for 1000 grain weight, But best correlation were noticed between NDVI and grain yield having R2 value 0.78, NDVI and 1000 grain wt having R2 value 0.80, LCI and grain yield having R2 value 0.82, LCI and 1000 grain weight having R2 value 0.79. The LCI and NDVI were having best correlation with grain yield and 1000 grain wt. It was concluded that amongst the various indices selected, Leaf color index (LCI) was the only index which was correlated better, with all the plant growth parameters and yield at booting stage of the wheat crop.
    No preview · Article · Sep 2015 · Journal of the Indian Society of Remote Sensing
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