Human papillomaviruses in intraepithelial neoplasia and squamous cell carcinoma of the conjunctiva: A study from Mozambique

ArticleinEuropean journal of cancer prevention: the official journal of the European Cancer Prevention Organisation (ECP) 22(6) · June 2013with169 Reads
DOI: 10.1097/CEJ.0b013e328363005d · Source: PubMed
Abstract
The infection with human papillomavirus (HPV) has been described as a risk factor for squamous cell carcinoma of the conjunctiva (SCCC), although the evidence is conflicting. To assess the relation between HPV infection and intraepithelial neoplasia or SCCC, we evaluated archived material from biopsies of the conjunctiva performed at the Maputo Central Hospital (Mozambique) in patients with suspected eye cancer. The quality of DNA was assessed by PCR using β-globin-specific primers. A total of 22 consecutive biopsies (intraepithelial neoplasia, SCCC, and benign conditions) positive for β-globin were further tested for HPV infection by PCR using the general primers GP5+/GP6+ and CPI/CPII. In addition, PCR with type-specific primers HPV 16 and HPV 18 was performed. Nineteen biopsies corresponded to intraepithelial neoplasia (two low-grade and nine high-grade) or SCCC (n=8), from which 11 (57.9%) tested positive for HPV infection; nine were positive for CPI/CPII, including one case also positive for GP5+/GP6+ and HPV 18, and the remaining two tested positive only for HPV 16. HPV DNA was not detected in any of the three biopsies of benign conditions. These results suggest a stronger association between infection with cutaneous HPV and SCCC than for mucosal HPV. However, further research is required to clarify the relation between HPV and SCCC as well as to understand the potential of the HPV vaccine currently available for cervical cancer to prevent SCCC.
    • "There are only few studies concerning HPV infection in women in Mozambique. Only one paper dealt with HPV infection in men and considers the HPV infection in squamous cell carcinoma of the conjunctiva showing a strong association [14]. Due to this lack of studies and the evidence of the impact of HPV related pathologies in male, it is mandatory to increase research on this topic. "
    [Show abstract] [Hide abstract] ABSTRACT: Human Papillomavirus is agent of the most common sexually transmitted disease which is able to infect mucosal and cutaneous membranes of the anogenital region, upper aerodigestive tract, and other head and neck mucosal regions. Although mainly HPV infection can be asymptomatic and transient, it may persist and give rise to various lesions such as warts, condyloma dysplasia and cancers depending on low or high risk type of HPV infection. Moreover, growing recent evidence suggests a role of this virus in male and female fertility. To date no effective prevention, test, treatment and control strategies are provided for people in developing countries despite the reported high incidence of HPV both in women and men. This paper reviews the more recent literature about HPV infection highlighting epidemiology, related pathologies and possible fertility effects of HPV in male and female with particular attention to the Mozambique context.
    Full-text · Article · May 2016
    • "More recently, de Koning et al (2008) presented, together with their case–control study, a systematic review of the literature on the association between HPV infection and SCCC, including studies published up to 2008. The present systematic review included six additional studies (Auw-Haedrich et al, 2008; Guthoff et al, 2009; AteenyiAgaba et al, 2010; Asadi-Amoli et al, 2011; Chauhan et al, 2012; Carrilho et al, 2013) published since then, although only one provided information that could be used to assess the association between infection with cutaneous subtypes of HPV and SCCC. Our study also adds to the previous reports the assessment of a potential interaction between HIV and HPV infections, although further research is needed for more robust conclusions on this issue, and the demonstration that the published evidence on the association between mucosal HPV and SCCC overestimates the true association. "
    [Show abstract] [Hide abstract] ABSTRACT: Background: The frequency of ocular surface squamous neoplasias (OSSNs) has been increasing in populations with a high prevalence of infection with human immunodeficiency virus/acquired immunodeficiency syndrome (HIV/AIDS) and infection with human papillomavirus (HPV). We aimed to quantify the association between HIV/AIDS and HPV infection and OSSN, through systematic review and meta-analysis. Methods: The articles providing data on the association between HIV/AIDS and/or HPV infection and OSSN were identified in MEDLINE, SCOPUS and EMBASE searched up to May 2013, and through backward citation tracking. The DerSimonian and Laird method was used to compute summary relative risk (RR) estimates and 95% confidence intervals (95% CI). Heterogeneity was quantified with the I2 statistic. Results: HIV/AIDS was strongly associated with an increased risk of OSSN (summary RR=8.06, 95% CI: 5.29–12.30, I2=56.0%, 12 studies). The summary RR estimate for the infection with mucosal HPV subtypes was 3.13 (95% CI: 1.72–5.71, I2=45.6%, 16 studies). Four studies addressed the association between both cutaneous and mucosal HPV subtypes and OSSN; the summary RR estimates were 3.52 (95% CI: 1.23–10.08, I2=21.8%) and 1.08 (95% CI: 0.57–2.05, I2=0.0%), respectively. Conclusion: Human immunodeficiency virus infection increases the risk of OSSN by nearly eight-fold. Regarding HPV infection, only the cutaneous subtypes seem to be a risk factor.
    Full-text · Article · Sep 2013
  • [Show abstract] [Hide abstract] ABSTRACT: Human papillomavirus (HPV) infection is strongly associated with several human cancers; the most known genotypes involved being HPV 16 and HPV 18. We report the detection of HPV 52 in a sample taken from a 47-year-old patient with squamous cell carcinoma of the conjunctiva of the left eye. The method used for the detection of HPV was real time polymerase chain reaction. The evolution was favorable after surgical removal of the tumor and the patient was explained that long-term follow-up is essential to avoid recurrence.
    Article · Feb 2015
Show more