Article

Neuroglobin expression in neurogenesis

Buck Institute for Research on Aging, 8001 Redwood Boulevard, Novato, CA 94945 USA.
Neuroscience Letters (Impact Factor: 2.03). 05/2013; 549. DOI: 10.1016/j.neulet.2013.04.039
Source: PubMed

ABSTRACT

Neuroglobin is a hypoxia-inducible, neuroprotective protein related to myoglobin and hemoglobin, but little is known about its neurodevelopmental expression or function. To begin to explore these issues, we measured neuroglobin protein expression during neuronal differentiation of human embryonic stem cells in vitro and in the neurogenic subventricular zone of adult rats in vivo. Neuroglobin protein expression was barely detectable by western blotting in human embryonic stem cells, but was readily demonstrable in neural stem cells, and was further induced upon differentiation to neurons. In the adult subventricular zone, neuroglobin expression coincided with that of the neuronal lineage marker doublecortin, but not with vimentin or glial fibrillary acidic protein. These findings suggest that neuroglobin is expressed early in the course of neuronal differentiation and may, therefore, have a role in neurodevelopment.

Download full-text

Full-text

Available from: Bryan Haines, Sep 03, 2015
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Neuroglobin is a hypoxia-inducible, neuroprotective protein related to myoglobin and hemoglobin, but little is known about its neurodevelopmental expression or function. To begin to explore these issues, we measured neuroglobin protein expression during neuronal differentiation of human embryonic stem cells in vitro and in the neurogenic subventricular zone of adult rats in vivo. Neuroglobin protein expression was barely detectable by western blotting in human embryonic stem cells, but was readily demonstrable in neural stem cells, and was further induced upon differentiation to neurons. In the adult subventricular zone, neuroglobin expression coincided with that of the neuronal lineage marker doublecortin, but not with vimentin or glial fibrillary acidic protein. These findings suggest that neuroglobin is expressed early in the course of neuronal differentiation and may, therefore, have a role in neurodevelopment.
    Full-text · Article · May 2013 · Neuroscience Letters
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Neuroglobin is a neuron specific hexacoordinated globin capable of binding various ligands, including O2, NO, and CO, the biological function of which is still uncertain. Various studies seem to indicate that neuroglobin is a neuroprotective agent when overexpressed, acting as a potent inhibitor of oxidative and nitrosative stress. In this study, we evaluated the pathophysiological response of the neuroglobin gene and protein expression in the cerebral tissue of rats sustaining a traumatic brain injury of different severity, whilst simultaneously measuring the oxidants/antioxidants balance. Two levels of trauma (mild and severe) were induced in anesthetized animals using the weight-drop model of diffuse axonal injury. Rats were then sacrificed at 6, 12, 24, 48 and 120 hours after traumatic brain injury, and the gene and protein expression of neuroglobin and the concentrations of malondialdehyde (as a parameter representative of ROS mediated damage), nitrite+nitrate (indicative of NO metabolism), ascorbate and GSH were determined in the brain tissue. Results indicated that mild traumatic brain injury, while causing a reversible increase in oxidative/nitrosative stress (increase in malondialdehyde and nitrite+nitrate) and imbalance in antioxidants (decrease in ascorbate and GSH), did not induce any change in neuroglobin. Conversely, severe traumatic brain injury caused an over 9-fold and 5-fold increase in neuroglobin gene and protein expressions respectively, as well as a remarkable increase in oxidative/nitrosative stress and depletion of antioxidants. The results of the present study, showing a lack of effect in mild traumatic brain injury, as well as an asynchronous time course changes of neuroglobin expression, oxidative/nitrosative stress and antioxidants in severe traumatic brain injury, do not seem to support the role of neuroglobin as an endogenous neuroprotective antioxidant agent, at least under pathophysiological conditions.
    Full-text · Article · Jan 2014 · Free Radical Biology and Medicine
  • [Show abstract] [Hide abstract]
    ABSTRACT: Neuroglobin is a brain globin with neuroprotective effects against ischemia and related pathological processes, acting as O2 sensor and antiapoptotic pathway transducer. Here, we survey data on neuroanatomical coexpression of transcription factors, epigenetic signature and predictive transcription factor binding sites at the neuroglobin gene locus to find hints of pathways to neuroglobin transcriptional regulation. These data provide a glimpse of how neuroglobin expression may translate into neuronal diversity and function, as well as disease. © 2014 IUBMB Life, 2014.
    No preview · Article · Feb 2014 · International Union of Biochemistry and Molecular Biology Life
Show more