1) Laboratoire d′Obtention de Substances Therapeutiques (LOST), Faculte des Sciences, Universite Mentouri -
Constantine, Campus Chaabet Ersas, 25000 Constantine, Algerie, Fax 213 31 63 53 52, e-mail: firstname.lastname@example.org;
2) Universite de Rennes 1, U.M.R./C.N.R.S. N 6509, Campus de Beaulieu, 35042 Rennes cedex, France. Published in Khimiya
Prirodnykh Soedinenii, No. 1, pp. 25-26, January-February, 2004. Original article submitted January 14, 2004.
2004 Plenum Publishing Corporation
Chemistry of Natural Compounds, Vol. 40, No. 1, 2004
CHEMICAL COMPOSITION OF THE ESSENTIAL OIL OF
Rosmarinus officinalis CULTIVATED IN THE ALGERIAN SAHARA
, A. Nacer
, A. Kabouche
, and C. Bruneau
The volatile compounds obtained by hydrodistillation of the aerial parts of
lgerian Sahara were analyzed by GC/MS. Thirty compounds were characterized representing 98.2%
of the essential oil with 1,8-cineole (29.5%), 2-ethyl-4,5-dimethylphenol (12.0%) and camphor (11.5%) as the
, essential oil, GC/MS.
is one of the oldest known medicinal plants in Algeria. It is used as an antispasmolytic and as a flavor
and fragrance ingredient in the food.
We identified 30 compounds in the hydrodistilled oil of
, cultivated at Oued Souf (Algerian
Sahara), with 1,8-cineole (29.5%), 2-ethyl-4,5-dimethylphenol (12.0%), camphor (11.5%), borneol (9.4%), (+)-
-pinene (7.5%), and camphene (5%) as the main components (Table 1). These results are in agreement with the
reported essential oils of Italian R. officinalis  mainly composed of 1,8-cineole (43.3%),
-pinene (18.6%), borneol (8.96%),
-pinene (6.79%), (+)-
-terpineol (3.59%), and the Spanish species  mainly represented by camphor (40.85%), 1,8-cineole
(12.20%), and borneol (7.62%).
GC analyses were performed using a Perkin–Elmer gas chromatograph equipped with two FID, a data handling system,
and a vaporizing injector port into which two columns of different polarities were installed: a DB-1 fused silica column
(30 m × 0.25 mm i.d., film thickness 0.25 mm) and a DB-Wax fused silica column (30 m × 0.25 mm i.d., film thickness
0.25 mm). Oven temperature was programmed, 45–175°C at 3°C min
, subsequently at 15°C min
up to 300°C, and then held
isothermal (15 min); carrier gas, He at 30 cm/min. GC chiral analyses were performed using a Perkin–Elmer gas chromatograph
equipped with a FID, a data handling system, a Cyclodex-B fused-silica column (30 m × 0.25 mm i.d., film thickness 0.25 mm),
and a DB-Wax fused silica column (30 m × 0.25 mm i.d., film thickness 0.25 mm). Oven temperature was 75°C, isothermal,
injector and detector temperatures, 230°C and 240°C, respectively; carrier gas, He at 42 cm/min.
GC-MS analyses were performed on a Perkin–Elmer apparatus equipped with a DB-1 fused silica column
(30 m × 0.25 mm i.d., film thickness 0.25 mm) and interfaced with an ion trap detector (ITD; software 4.1). Injector temperature
MS operating parameters were as follows: ion trap temperature, 220°C; split ratio1:40; ionization potential, 70 e
current, 60 mA; scan range, 40–300 a.m.u, scan time, 1 s.
Identification of components was done by comparison of the retention indices (RI) relative to C
MS with the corresponding database (NIST library) and with mass spectral literature [3–5]. Relative percentage amounts of the
identified components were calculated from the total ion chromatograms by a computerized integrator.