Effects of a Chayotte (Sechium edule) Extract (Macerated) on the Biochemistry of Blood of Wistar Rats and on the Action Against the Stannous Chloride Effect
Universidade do Estado do Rio de Janeiro, Instituto de Biologia Roberto Alcantara Gomes, Departamento de Biofisica e Biometria. Av. 28 de setembro, 87, Rio de Janeiro, RJ 20551-030, Brazil.Pakistan Journal of Biological Sciences 04/2007; 10(5):823-7. DOI: 10.3923/pjbs.2007.823.827
The use of natural products as medicines has been growing in the entire world. There are concerns that these products may contain potentially toxic ingredients and contaminants such as heavy metals. The labeling of blood constituents with technetium-99m has been influenced by the presence of natural extracts. We evaluated the influence of a chayotte (Sechium edule) extract (100% v/v macerated) on the labeling of blood elements with 99mTc. The animals were treated with the extract during 15 days. Samples of blood were carried out with specific blood biochemistry kits. The present study analyzed the influence ofchayotte in the survival of the strain of Escherichia coli AB1157 submitted to reactive oxygen species induced by stannous chloride. There was a reduction of the lethal effect induced by stannous chloride on the survival of the E. coli culture in the presence of chayotte. The results indicated a decrease in the level of glucose and globulin The effect of the extract could be explained by its metabolic transformation inducing the generation of oxidant metabolites. The culture of bacteria when was treated with stannous chloride and chayotte simultaneously, the extract could be reacting with stannous chloride ions, protecting them against the oxidation avoiding the generation of reactive oxygen species.
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ABSTRACT: Scientists have reported that synthetic or natural drugs can interfere with the labeling of blood constituents with 99mTc. Drugs can alter the morphology of red blood cells. An increasing number of people in the world are using natural products. In this study it was evaluated the influence of a chayotte extract on the morphology of red blood cells and on the radiolabeling of blood elements with technetium-99m (99m Tc). Blood was withdrew from Wistar rats and treated with chayotte, and then it was incubated with stannous chloride and 99mTc. The blood smears were prepared. It was observed that the extract was capable of altering the morphology of red blood cells. The effect of the extract could be explained by its stabilizing activity in the red blood cell membrane as well as its antioxidant effect due to the radiolabeling process which has not been altered.
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