From MEFs to Matrigel 2: splitting hESCs from MEFs onto Matrigel

David Geffen School of Medicine, University of California, Los Angeles, USA.
Journal of Visualized Experiments (Impact Factor: 1.33). 02/2008; pii(16). DOI: 10.3791/831
Source: PubMed


This video demonstrates how to grow human embryonic stem cells (hESCs) on mouse embryonic fibroblast (MEF) feeder cells, how to passage hESCs from MEF plates to feeder cell-free Matrigel plates.

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