Using Ultradeep Pyrosequencing to Study HIV-1 Coreceptor Usage in Primary and Dual Infection

University of California San Diego, La Jolla, California, USA.
The Journal of Infectious Diseases (Impact Factor: 6). 04/2013; 208(2). DOI: 10.1093/infdis/jit168
Source: PubMed


HIV-1 dual infection (DI) and CXCR4 (X4) coreceptor usage are associated with accelerated disease progression but frequency
and dynamics of coreceptor usage during DI is unknown. Ultradeep sequencing was used to interrogate for DI and infer coreceptor
usage in longitudinal blood samples of 102 subjects. At baseline, X4 usage was high (23 subjects harbored X4 variants) and
was not associated with infection duration or DI. Coreceptor usage changed over time in 12 of 47 participants, and X4 usage
emerged in 4 of 41 monoinfections vs 2 of 5 superinfections (P = .12), suggesting a weak statistical trend toward occurrence of superinfection and acquiring X4 usage.

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Available from: Sheldon Robert Morris, Feb 05, 2016
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