This study was carried out during 2020-2021 growing season on twelve years old olive trees at the orchard of Horticulture Department, College of Agricultural Engineering Sciences, University of Sulaimani, Bakrajo, Kurdistan Region, Iraq. The objective of the study was to investigate the effects of artificial pollination and boron spray application on yield and fruit quality of two olive cultivars "Sorani" and "Khodeiri" and to identify self-incompatibility index and determination of suitable pollinizers. Six selective pollinizers (Arbequina, Ashrasi, Hojiblanca, K18, Kaissy and Picual), in addition to open pollination, and lastly self-pollination as control, and two levels of boron (0 and 200 mg.L-1), as well as two main cultivars (Sorani and Khodeiri) were used. The experiment was laid out as a factorial experiment using a randomized complete block design (RCBD) with six replicates and the means were compared according to Duncan's multiple range test (P≤ 0.05).
The results showed that Sorani and Khodeiri olive cultivars are partially self-incompatible, with self-incompatibility indices of (0.59 and 0.51), respectively. Sorani cultivar gave the highest percentage of final fruit set (1.96%), fruit weight (2.64 g), fruit size (2.51 cm3), fruit length (20.54 mm), fruit diameter (15.35 mm), olive flesh weight (1.78 g), olive pit length (15.73 mm), olive pit size (0.74 cm³), fruit dry matter (49.00%), total soluble solids (31.26%), oil content percentage (38.81%), the lowest oil acidity (0.46%), the lowest peroxide value (0.46 meq.kg-1 oil), leaf chlorophyll content (73.77 SPAD), contents of leaf soluble sugar (185.74 μg.g-1), phosphorus (0.49%). While, the highest contents of leaf total phenols (266.06 μg GAE.g-1), nitrogen (1.93%), calcium (0.52%), magnesium (0.44%) and boron concentration (6.62 mg.L-1) were obtained from Khodeiri cultivar.
Boron sprayed with 200 mg.L-1 achieved the highest values of all studied parameters. Results highlight the importance of olive cross-pollination for obtaining satisfactory final fruit set (2.21%) in comparison with self-pollination. Ashrasi pollinizer caused the superior values of initial fruit set (25.52%), olive pit diameter (9.39 mm), fruit dry matter (51.59%), total soluble solids (31.19%), leaf chlorophyll content (76.07 SPAD), leaf total phenols (265.70 μg GAE.g-1), phosphorus (0.53%), potassium (2.22%), magnesium (0.56%), boron (9.55 mg.L-1). At the same time, Arbequina pollinizer exhibited the highest values of fertility index (1.58), final fruit set (2.21%), the least value of oil acidity (0.42%), soluble sugar content (198.90 μg.g-1 DW) and nitrogen content (2.15%). Furthermore, Picual pollinizer resulted in the highest value of fruit size (2.67 cm3), olive flesh weight (1.85 g), olive leaf calcium content (0.65%) and the minimum peroxide value (4.73 meq.kg-1 oil). However, Hojiblanca pollinizer significantly resulted in the maximum values of fruit weight (2.72 g), fruit length (18.77 mm), fruit diameter (15.76 mm), olive pit weight (0.84 g), pit length (15.94 mm), pit size (0.79 cm³) and oil content (38.59%). In contrast, the highest value of self-incompatibility (0.60) was obtained from Kaissy pollinizer.
The combination of the two cultivars, Sorani and Khodeiri with 200 mg.L-1 boron increased the favourable measurements and decreased unfavourable characteristics. Arbequina, Ashrasi Hojiblanca and Picual were the best pollinizers for Sorani cultivar, but Khodeiri cultivar gave the best results with Arbequina, Ashrasi and Picual pollinizers. Also, the best outcomes were observed as 200 mg.L-1 boron interacted with Arbequina, Ashrasi, Hojiblanca and Picual pollinizers.
The interaction among the three factors showed that Sorani cultivar interacted with 200 mg.L-1 boron and Arbequina, Ashrasi, Hojiblanca and Picual pollinizers gave the best results. In contrast, the interaction of Khodeiri cultivar, 200 mg.L-1 boron and each Picual and Ashrasi pollinizers gave the best consequences in some studied parameters. Generally, Sorani cultivar was more responsive to 200 mg.L-1 boron and pollinizers than Khodeiri cultivar. Boron at 200 mg.L-1 was outstanding to improve growth and fruit characteristics of Sorani and Khodeiri cultivars. Additionally, Arbequina, Ashrasi, Hojiblanca and Picual were the best pollinizers for Sorani and Khodeiri cultivars.