Synthesis and Labeling of RNA In Vitro

ArticleinCurrent protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.] Chapter 4:Unit4.15 · April 2013with10 Reads
DOI: 10.1002/0471142727.mb0415s102 · Source: PubMed


    This unit discusses several methods for generating large amounts of uniformly labeled, end-labeled, and site-specifically labeled RNAs in vitro. The methods involve a number of experimental procedures, including RNA transcription, 5' dephosphorylation and rephosphorylation, 3' terminal nucleotide addition (via ligation), site-specific RNase H cleavage directed by 2'-O-methyl RNA-DNA chimeras, and 2-piece splint ligation. The applications of these RNA radiolabeling approaches are also discussed. Curr. Protoc. Mol. Biol. 102:4.15.1-4.15.14. © 2013 by John Wiley & Sons, Inc.