Silica nanoparticles as a tool for fluorescence collection efficiency enhancement

Nanoscale Research Letters (Impact Factor: 2.78). 03/2013; 8(1):146. DOI: 10.1186/1556-276X-8-146
Source: PubMed


In this work we demonstrate enhancement of the fluorescence collection efficiency for chlorophyll-containing photosynthetic complexes deposited on SiO2 spherical nanoparticles. Microscopic images of fluorescence emission reveal ring-like emission patterns associated with chlorophyll-containing complexes coupled to electromagnetic modes within the silica nanoparticles. The interaction leaves no effect upon the emission spectra of the complexes, and the transient behavior of the fluorescence also remains unchanged, which indicates no influence of the silica nanoparticles on the radiative properties of the fluorophores. We interpret this enhancement as a result of efficient scattering of electromagnetic field by the dielectric nanoparticles that increases collection efficiency of fluorescence emission.

Download full-text


Available from: Bartłomiej J Jankiewicz
  • Source
    • "Nanoparticles (NPs), based on pure crystalline silica (Si), are capable of fluorescence detection, which makes them applicable as a biological probe [1]. Their high biocompatibility allows these particles to be considered as candidates for providing direct drug delivery [2]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Silica-based nanoparticles (NPs) pose great potential for medical and biological applications; however, their interactions with living cells have not been investigated in full. The objective of this study was to analyze the mechanical characteristics of mesenchymal stem cells when cultured in the presence of silica (Si) and silica-boron (SiB) nanoparticles. Cell stiffness was measured using atomic force microscopy; F-actin structure was evaluated using TRITC-phalloidin by confocal microscopy. The obtained data suggested that the cell stiffness increased within the following line: 'Control' - 'Si' - 'SiB' (either after 1-h cultivation or 24-h incubation). Moreover, the cell stiffness was found to be higher after 1-h cultivation as compared to 24-h cultivation. This result shows that there is a two-phase process of particle diffusion into cells and that the particles interact directly with the membrane and, further, with the submembranous cytoskeleton. Conversely, the intensity of phalloidin fluorescence dropped within the same line: Control - Si - SiB. It could be suggested that the effects of silica-based particles may result in structural reorganization of cortical cytoskeleton with subsequent stiffness increase and concomitant F-actin content decrease (for example, in recruitment of additional actin-binding proteins within membrane and regrouping of actin filaments).
    Full-text · Article · Jun 2014 · Nanoscale Research Letters
  • Source
    • "PCPs on silver island films [132], semicontinuous silver films [133]; spherical gold nanoparticles [10, 134], silver nanowires [135] silica nanoparticles [136] have been reported. The topic has been recently reviewed [137, 138] so here we just underline its strong potential in a technological perspective, both in the field of hybrid nanostructures for light energy conversion, and as a tool for emission enhancement of PCPs as a fluorophore. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Peridinin-Chlorophyll-a-Proteins (PCPs) are water-soluble light harvesting complexes from dinoflagellates. They have unique light-harvesting and energy transfer properties which have been studied in details in the last 15 years. This review aims to give an overview on all the main aspects of PCPs photophysics, with an emphasis on some aspects which have not been reviewed in details so far, such as vibrational spectroscopy studies, theoretical calculations, and magnetic resonance studies. A paragraph on the present development of PCPs towards technological applications is also included.
    Full-text · Article · Mar 2014 · Current Protein and Peptide Science
  • Source

    Full-text · Article · Jan 2015