Genomics of Loa loa, a Wolbachia-free filarial parasite of humans

Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
Nature Genetics (Impact Factor: 29.35). 03/2013; 45(5). DOI: 10.1038/ng.2585
Source: PubMed


Loa loa, the African eyeworm, is a major filarial pathogen of humans. Unlike most filariae, L. loa does not contain the obligate intracellular Wolbachia endosymbiont. We describe the 91.4-Mb genome of L. loa and that of the related filarial parasite Wuchereria bancrofti and predict 14,907 L. loa genes on the basis of microfilarial RNA sequencing. By comparing these genomes to that of another filarial parasite, Brugia malayi, and to those of several other nematodes, we demonstrate synteny among filariae but not with nonparasitic nematodes. The L. loa genome encodes many immunologically relevant genes, as well as protein kinases targeted by drugs currently approved for use in humans. Despite lacking Wolbachia, L. loa shows no new metabolic synthesis or transport capabilities compared to other filariae. These results suggest that the role of Wolbachia in filarial biology is more subtle than previously thought and reveal marked differences between parasitic and nonparasitic nematodes.

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Available from: Jennifer Russo Wortman, Apr 02, 2014
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    • "wCte Ctenocephalides felis SRR1222150 Raw data – – – B [36] wOo Onchocerca ochengi HE660029 Complete 1 957,990 32.1 C [24] wOv Onchocerca volvulus strain Cameroon HG810405 Complete 1 960,618 32.1 C Cotton et al. (unpublished) wDi Dirofilaria immitis PRJEB4154 a WGS 2 921,012 32.7 C [22] wBm strain TRS Brugia malayi AE017321 Complete 1 1,080,084 34.2 D [35] wBn Wuchereria bancrofti ADHD00000000 WGS 763 1,052,327 34.0 D [26] wLs Litomosoides sigmodontis PRJEB4155 a WGS 10 1,048,936 32.1 D [22] wFol Folsomia candida SRR1222159 Raw data – – – E [36] wCle Cimex lectularius AP013028 Complete 1 1,250,060 36.3 F [69] wOc Osmia caerulescens SRR1221705 Raw data – – – F [36] wMen Mengenilla moldrzyki SRX095325 WGS – – – F [68] "
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