Microlocalization of lipophilic porphyrins: Non-toxic enhancers of boron neutron-capture therapy

ArticleinInternational Journal of Radiation Biology 89(8) · March 2013with18 Reads
DOI: 10.3109/09553002.2013.782446 · Source: PubMed
Purpose: To compare the macroscopic and microscopic distributions of the novel non-toxic lipophilic porphyrins copper (II) 5,10,15,20-tetrakis-(3-[1,2 dicarba-closo-dodecaboranyl]methoxyphenyl)-porphyrin (CuTCPH), potentially useful for boron neutron-capture therapy (BNCT), with those of its zinc fluorescent congener zinc (II) 5,10,15,20-tetrakis-(3-[1,2 dicarba-closo-dodecaboranyl]methoxyphenyl)-porphyrin (ZnTCPH) in tissues of tumor-bearing mice. Materials and methods: ZnTCPH and CuTCPH were synthesized, then injected intraperitoneally (ip) into tumor-bearing mice. Macroscopic biodistribution was assessed by determining average boron concentrations in tumor, blood, brain, skin, and liver using atomic-emission spectrometry. The euthanized mice and their vital organs were photographed first under an ultraviolet lamp and then under a bright fluorescent lamp. Thin sections of liver and tumor were analyzed by confocal fluorescence microscopy (CFM). Results: ZnTCPH-injected, but not CuTCPH-injected mice bearing subcutaneous tumors showed fluorescence from liver, spleen and tumors. Macrodistributions of boron in various tissues were similar in mice whether injected with ZnTCPH or CuTCPH. CFM of unfixed liver sections showed cytoplasmic fluorescence from Kupffer cells in a periportal lobular distribution evenly throughout the liver. In the tumors studied, such fluorescence was also cytoplasmic but unlike liver fluorescence, was macroscopically heterogeneous. Conclusion: ZnTCPH serves as a useful fluorescent experimental surrogate for CuTCPH to delineate its macroscopic and microscopic distributions in organs and tumors.
  • [Show abstract] [Hide abstract] ABSTRACT: Nanotechnology is rapidly growing with its advancements in treating medical ailments. The various physical, chemical and biological method of synthesizing nanomaterials has led to delightful evidences of creating new bio remedies. Even though physical and chemical methods cost high, they produce worthy results and contribute to the research. More evidences on biological method tend to be easily heading forth the recent research. In the segment of modern research, they pay attention to the cancer nanotechnology finding betterment in the cancer research. The mechanisms that tumor evolves and destroys the neighbour tissues is a contradictory in all organs. The state of curing it still is a controversy proving it in vitro and in vivo. Targeting the tumor cells with nanoparticles gains much more attention now. This strategy of developing nanoparticles will make changes with the antioxidant and free radical system, which has its unique role in ROS activating systems. Most of the nanoparticle synthesis has been shown to reduce toxicity of the compounds in the yield of nanomaterials. There is a focus on comparing these nanomaterial syntheses with the metal resources of silver, gold, copper, zinc, aluminium elements. There are differences in the material output with various metals that generate structural compartmentation leading to limitations and advantages of the nanomaterial synthesized. When taking into account the gold and the zinc nanoparticles, there is recent research work emphasized as topmost of the nanoworld. Of that, zinc nanomaterial is said to be worth considering as the best nanoparticle, which possess various advancing properties that attract researchers to utilize it with current technologies. Hence the top two nanoparticles of gold and zinc has been given importance here and their advantages, methods of which they coluld be brought out with improved results are reviewed. The current research in 2014 would focus on the biological method of synthesizing nanoparticles with eminent gold and zinc metals. There are many provoking ideas of work discussed in this review that are indulged with gold and zinc nanoparticles which makes it interesting to emerge with wonders in research and betterment towards the world of nanomedicine.
    Article · Aug 2014