Article

Determination of Efficacy of Anvirzel ™ in 37 Established Cancer Cell Lines

INTERNATIONAL PHARMACEUTICAL INDUSTRY 08/2011; 3(3):68-72.

ABSTRACT

The present study investigates the efficacy of Anvirzel™, an extract of Nerium oleander, in more than 35 different human cancer cell lines, including all the types of tumours. Assays that have been used in order to measure the efficacy of Anvirzel™ in 24, 48 and 72h of incubation in 37 different cancer cell lines, were viability assays [Methyl-Tetrazolium dye (MTT), Crystal violet staining (CVE) and SulfoRodhamine B (SRB)]. In colon, lung, uterus and breast cancer cell lines, reduction of the null has been shown, up to 70% by type. All studies to date have demonstrated the efficacy of Anvirzel™ in individual cancers, such as prostate, pancreatic and myeloma cell lines. The present study demonstrates that this drug has a wider field of action, also in other cancer cell lines. It would be preferable to conduct studies concluding the interaction between Anvirzel™ and other drugs that are used already for the treatment of these cancers. Introduction Anvirzel™ is an extract of Nerium oleander that consists mainly of oleandrin and another cardiac glycoside, oleandrigenin. Oleandrin has been used for the treatment of patients with heart failure. Many other studies have demonstrated that it also has an anti-proliferative action, so it can be used for the treatment of tumours 1,2,3,4 . But the major problem is the toxicity of oleandrin in normal cells and tissues 5,6

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    • "Anvirzel™ is an extract of Nerium oleander comprised primarily of Oleandrin and Oleandrigenin [8]. Recent studies demonstrate that Anvirzel™ decreases viability in prostate cancer cell lines as well as a wide range of other human cancer cell lines [9-12]. Cisplatin (CDDP) is a platinum-based chemotherapy drug used to treat various types of cancer [13,14]. "
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    ABSTRACT: Background Platinum derivatives are used widely for the treatment of many cancers. However, the toxicity that is observed makes imperative the need for new drugs, or new combinations. Anvirzel™ is an extract which has been demonstrated with experimental data that displays anticancer activity. The aim of the present study is to determine whether the combination of Cisplatin and Anvirzel™ has a synergistic effect against different types of cancer. Materials and methods To measure the efficacy of treatment with Cisplatin and Anvirzel™, methyl-tetrazolium dye (MTT) chemosensitivity assays were used incorporating established human cancer cell lines. Measurements were performed in triplicates, three times, using different incubation times and different concentrations of the two formulations in combination or on their own. t-test was used for statistical analysis. Results In the majority of the cell lines tested, lower concentrations of Anvirzel™ induced a synergistic effect when combined with low concentrations of Cisplatin after an incubation period of 48 to 72 h. The combination of Anvirzel™/Cisplatin showed anti-proliferative effects against a wide range of tumours. Conclusion The results showed that the combination of Anvirzel™ and Cisplatin is more effective than monotherapy, even when administered at low concentrations; thus, undesirable toxic effects can be avoided.
    Full-text · Article · Mar 2013 · BMC pharmacology & toxicology
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    ABSTRACT: Background Platinum derivatives are used widely for the treatment of many cancers. However, the toxicity that is observed makes imperative the need for new drugs, or new combinations. AnvirzelTM is an extract which has been demonstrated with experimental data that displays anticancer activity. The aim of the present study is to determine whether the combination of Cisplatin and AnvirzelTM has a synergistic effect against different types of cancer. Materials and methods To measure the efficacy of treatment with Cisplatin and AnvirzelTM, methyl-tetrazolium dye (MTT) chemosensitivity assays were used incorporating established human cancer cell lines. Measurements were performed in triplicates, three times, using different incubation times and different concentrations of the two formulations in combination or on their own. t-test was used for statistical analysis. Results In the majority of the cell lines tested, lower concentrations of AnvirzelTM induced a synergistic effect when combined with low concentrations of Cisplatin after an incubation period of 48 to 72 h. The combination of AnvirzelTM/Cisplatin showed anti-proliferative effects against a wide range of tumours. Conclusion The results showed that the combination of AnvirzelTM and Cisplatin is more effective than monotherapy, even when administered at low concentrations; thus, undesirable toxic effects can be avoided.
    Full-text · Article · Mar 2013 · BMC Pharmacology