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Clarification of Peziza fimeti with notes on P. varia collections on dung

Authors:
Gianfranco Medardi at AMB - Associazione Micologica Bresadola
Gianfranco Medardi
  • AMB - Associazione Micologica Bresadola
Angela Lantieri
Angela Lantieri
Donald Pfister at Harvard University
Donald Pfister
Katherine F. LoBuglio
Katherine F. LoBuglio
Katherine F. LoBuglio
  • This person is not on ResearchGate, or hasn't claimed this research yet.
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Abstract and Figures

The smooth-spored species inhabiting dung, mainly of the Peziza fimeti group, were studied morphologically and through ITS sequence comparison. The results established that Peziza varia is also able to fruit on dung, clarifying a long-standing situation regarding two conflicting interpretations given in P. fimeti literature.
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Content uploaded by Angela Lantieri
Author content
All content in this area was uploaded by Angela Lantieri
Content may be subject to copyright.
  
MYCOTAXON
 
Volume 121, pp. 465–476 July–September 2012
Clarification of Peziza fimeti with notes on
P. varia collections on dung



1Via Giuseppe Mazzini 21, I-25086 Rezzato (Brescia), Italy
2Department of Biological, Geological and Environmental Sciences, Section of Plant Biology,
University of Catania, Antonino Longo 19, I-95125 Catania, Italy
3Department of Organismic and Evolutionary Biology and the Farlow Herbarium,
Harvard University, 22 Divinity Ave, Cambridge, MA 02138
4Via Goito 25, I-25127 Livorno, Italy
*angelalantieri@gmail.com
Peziza fimeti

Peziza varia
P. fimeti
 Pezizales
Introduction
         Peziza fimeti

P. fimeti
 P. fimetaria Humaria fimetaria


    
          
  −−−−−− 


 

          −− 
−−

Peziza fimeti
   

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

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 
 




Ursus ursus








fimeti
 fimeti 

P. fimeti
−
−  P. fimeti

   
        
   Peziza alcis
           
Peziza
Materials & methods
Material studied

Peziza
fimeti
  Peziza alcis ESTONIA JôgevamaaCo  
       
P. fimetiRägavereComm
Peziza fimeti P. varia... 
BetulaPicea 
  P. fimetiFINLAND Lapland  
   P. fimeti
Uusimaa  
AlcesUSAAlaska

P. fimeti
Peziza fimeti GERMANY Rheinland-Pfalz     
        Humaria fimeti 
ITALYAbruzzo
        FriuliVeneziaGiulia
 
Lazio  P.
vesiculosaLombardia
            
    P. granularis     
 
         
Piemonte

P. granularis
TrentinoAltoAdige       

    
 P. granularis
 
Veneto
 
P. granularis
P. granularis
        Toscana    
     P. vesiculosaRUSSIA Kamchatka
Ursus ursus
Ursus ursus
        Krasnodar 


     Kunashir Island   
         Respublika Tyva
          
TAJIKISTANRamit StateNature Reserve
 UZBEKISTAN
Juniperus

Peziza varia     P. fimeti   
ITALYFriuliVenezia Giulia
            P.
vesiculosa
P. vesiculosa
P. vesiculosaTrentinoAltoAdige

P. fimetiVeneto

      P.vesiculosaRUSSIAKamchatka
Ursus ursus
   P. fimeti YamaloNenetskiy Avtonomnyy Okrug

 P. fimeti
Morphological studies
         


           
       

DNA isolation, PCR, and sequencing techniques
 
   

 
            


        
           
           

DNA sequence analyses

             

Peziza fimetiPeziza
     




         

   

  

       
Peziza ampelina
Peziza fimeti P. varia... 
     Peziza      
   


          Peziza fimeti 

 P.subcitrina

Results & discussion
    
P. vesiculosaP. fimeti

 Peziza varia
           

P. varia

Peziza varia
       



  
          
    Peziza       


Peziza varia 
         

            
  
−−−−

Peziza varia
                 

Peziza fimeti P. varia... 

  Peziza alcis.         


      −
 −  
 −−    
−
        −   

P. varia


            

P. varia      
          Ursus   

    
P. fimeti
           
 P. varia 

Peziza variaP. alcis

Peziza alcis
   −  −     
−
Peziza fimeti P. varia... 
 Peziza fimeti.      

P. alcis 
P. varia

Peziza fimeti,


P. varia−−−

−
P. fimetiP. vesiculosa

     P. fimeti   
P. varia
          
P. ampliata
         

−
P. ampliata


P. variaP. arvernensis P. echinospora 


Acknowledgments



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


Literature cited

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


             
          

Peziza fimeti




Ascomycetes.
Pezizales

Peziza
Peziza

Peziza

Peziza





 Pezizales 



           
           

Peziza fimeti P. varia... 
     

Peziza fimeti

       Ascomycetes    



           
Phillipsia         

Peziza
     

            
Peziza          



Pezizales
Pezizales
 Peziza 



Pezizales


TalaromycesPenicillium
Biverticillium
Medeolaria farlowii
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
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        
   

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         
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
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      



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      
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

PezizaAleuriaGalactinia


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
 

... The analyses were congruent with each other in the suggested topology ( Fig. 1) and similar to the phylogenetic relationships already reported for this group (Hansen et al. 2002, Medardi et al. 2012. ...
... Hansen et al. (2002) noted that both P. domiciliana and P. arvernensis could be confused with P. varia. However, the spores in P. varia were observed by Medardi et al. (2012) as slightly smaller (14-16(-17.5) × 9-11(-12) µm), and the ornamentation in P. varia, very difficult to observe with a conventional light microscope in either surface or outline view, is readily visible in P. domiciliana and P. arvernensis. ...
Morphological and phylogenetic clarification of Peziza arvernensis, P. pseudovesiculosa, P. pseudosylvestris , and P. domiciliana
Article
  • Dec 2016
The concepts of the morphologically similar species Peziza arvernensis, P. pseudovesiculosa, P. pseudosylvestris, and P. domiciliana are revisited in light of new morphological and molecular data from freshly collected and herbarium specimens. Phylogenetic inference based on ITS nrDNA sequences suggests that P. arvernensis, P. pseudovesiculosa, and P. pseudosylvestris are monophyletic and that P. domiciliana represents an independent taxon.
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... The subsequent works on Peziza using molecular data, focused either on Peziza s. str. (MEDARDI et al., 2012;LANTIERI et al., 2016;PFISTER et al., 2016;VIZZINI et al., 2016;VIZZINI et al., 2020) or on other groups (MEDARDI et al., 2014b;LOIZIDES et al., 2017;VAN VOOREN et al., 2017;AG-NELLO et al., 2018;VAN VOOREN et al., 2018) did not change significantly the results of HANSEN et al. (2001HANSEN et al. ( , 2005, but help us to better understand the affinities among some species or to identify new taxa. Despite this, few nomenclatural changes have been made in the clades that do not belong to Peziza s. str. ...
Reinstatement of old taxa and publication of new genera for naming some lineages of the Pezizaceae (Ascomycota)
Article
Full-text available
  • Jul 2020
Based on both molecular data obtained from databases and from new studies of type collections of species of Peziza described by Donadini and morphological characters, taxonomical changes are proposed. Some names are given based on lineages of the Pezizaceae previously assigned to Peziza. A new multigene phylogeny (LSu, RPB2, β-tubulin) is provided. The following genera are reinstated: Daleomyces Setch., with D. gardneri (syn. Peziza proteana f. sparassoides) as type species, Geoscypha (Cooke) Lambotte, with Peziza violacea as type species, and Phaeopezia Sacc. ex Vido, with Peziza apiculata as type species. Peziza sect. Pur-pureodiscus, with Peziza subisabellina as type species, is raised to the generic rank. Six new genera are proposed: Ionopezia, with Peziza gerardii as type species, Malvipezia, with Peziza howsei as type species, Elaiopezia, with Galactinia polaripapulata as type species, Paragalactinia, with Peziza succosa as type species, Phylloscypha, with Peziza phyllogena as type species, and Legaliana, with Peziza badia as type species. The genera Galactinia, Heteroplegma, Infundibulum, Iotidea, Paramitra and Podaleuris are also discussed. The amy-loid reaction of asci is also discussed and characterised with three new categories inspired by those of Hansen et al. (2001).
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... pseudoammophila var. bonii (?)^, but it turned out to be P. varia, confirming that P. varia can fruit in coastal environments, in addition to the wide range of substrates described by Medardi et al. (2012). ...
Phylogeny and morphology of the Peziza ammophila complex (Pezizales, Ascomycota), with description of two new species and a new form
Article
Full-text available
  • Jul 2016
This study was focused on species of Peziza belonging to the “P. ammophila” complex, using both morphological and molecular approaches. Molecular and morphological analyses showed that several taxa are hidden under the name “ammophila” and, as a consequence, two additional species and one form are established: P. hellenica sp. nov., P. oceanica sp. nov. and P. ammophila f. megaspora f. nov. On the basis of the data that we have, these taxa seem to be related to some geographical areas; P. ammophila appears to be prevalent in northern, central and southern Europe, its f. megaspora is distributed in France and in the Netherlands, P. hellenica comes from Greece and P. oceanica from New Zealand. A cryptic species, not yet circumscribed satisfactorily, is described as P. deceptiva ad int. Moreover, the problem concerning the identification of P. ammophila is raised, the species is epitypified with a sequenced collection from Sicily (Italy) and a key to the species of the complex is provided.
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A reexamination and realignment of Peziza sensu lato (Pezizomycetes) species in southern South America
Article
Full-text available
  • Jun 2022
In this study we review recent collections and historical records of epigeous members of the Pezizales formerly placed in the large, heterogenous genus Peziza from temperate southern South America. Recent analyses using molecular phylogenetic methods allow placement of these species in several previously described genera in recognition of the heterogeneity of Peziza. We include species in nine genera, describe one new species (Peziza gamundiae sp. nov.), and propose one new combination (Phylloscypha nothofageti comb. nov.). We also demonstrate that Pustularia microspora is a synonym of the previously described taxon Peziza pseudosylvestris. Our purpose is to draw attention to these taxa in order to promote their collection and study in a modern framework.
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Validation de Peziza martinicensis sp. nov. (Pezizales)
Article
Full-text available
  • Apr 2020
Validation with molecular data of a Peziza s. str. species, already presented by the author in 2008 under the provisional name P. martinicae, characterized by a layer of gelatinized hyphae in the outermost part of the excipulum.
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Revision of some Peziza collections in herb. TAAM (Tartu)
Article
  • Dec 2016
Forty samples representing the genus Peziza kept in the Mycological Herbarium, Tartu, Estonia (TAAM) were studied. Collection data and taxonomic comments are presented.
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Fungal diversity notes 367-490: taxonomic and phylogenetic contributions to fungal taxa
Article
  • Sep 2016
This is a continuity of a series of taxonomic papers where materials are examined, described and novel combinations are proposed where necessary to improve our traditional species concepts and provide updates on their classification. In addition to extensive morphological descriptions and appropriate asexual and sexual connections, DNA sequence data are also analysed from concatenated datasets (rDNA, TEF-α, RBP2 and β-Tubulin) to infer phylogenetic relationships and substantiate systematic position of taxa within appropriate ranks. Wherever new species or combinations are being proposed, we apply an integrative approach (morphological and molecular data as well as ecological features wherever applicable). Notes on 125 fungal taxa are compiled in this paper, including eight new genera, 101 new species, two new combinations, one neotype, four reference specimens, new host or distribution records for eight species and one alternative morphs. The new genera introduced in this paper are Alloarthopyrenia, Arundellina, Camarosporioides, Neomassaria, Neomassarina, Neotruncatella, Paracapsulospora and Pseudophaeosphaeria. The new species are Alfaria spartii, Alloarthopyrenia italica, Anthostomella ravenna, An. thailandica, Arthrinium paraphaeospermum, Arundellina typhae, Aspergillus koreanus, Asterina cynometrae, Bertiella ellipsoidea, Blastophorum aquaticum, Cainia globosa, Camarosporioides phragmitis, Ceramothyrium menglunense, Chaetosphaeronema achilleae, Chlamydotubeufia helicospora, Ciliochorella phanericola, Clavulinopsis aurantiaca, Colletotrichum insertae, Comoclathris italica, Coronophora myricoides, Cortinarius fulvescentoideus, Co. nymphatus, Co. pseudobulliardioides, Co. tenuifulvescens, Cunninghamella gigacellularis, Cyathus pyristriatus, Cytospora cotini, Dematiopleospora alliariae, De. cirsii, Diaporthe aseana, Di. garethjonesii, Distoseptispora multiseptata, Dis. tectonae, Dis. tectonigena, Dothiora buxi, Emericellopsis persica, Gloniopsis calami, Helicoma guttulatum, Helvella floriforma, H. oblongispora, Hermatomyces subiculosa, Juncaceicola italica, Lactarius dirkii, Lentithecium unicellulare, Le. voraginesporum, Leptosphaeria cirsii, Leptosphaeria irregularis, Leptospora galii, Le. thailandica, Lindgomyces pseudomadisonensis, Lophiotrema bambusae, Lo. fallopiae, Meliola citri-maximae, Minimelanolocus submersus, Montagnula cirsii, Mortierella fluviae, Muriphaeosphaeria ambrosiae, Neodidymelliopsis ranunculi, Neomassaria fabacearum, Neomassarina thailandica, Neomicrosphaeropsis cytisi, Neo. cytisinus, Neo. minima, Neopestalotiopsis cocoës, Neopestalotiopsis musae, Neoroussoella lenispora, Neotorula submersa, Neotruncatella endophytica, Nodulosphaeria italica, Occultibambusa aquatica, Oc. chiangraiensis, Ophiocordyceps hemisphaerica, Op. lacrimoidis, Paracapsulospora metroxyli, Pestalotiopsis sequoiae, Peziza fruticosa, Pleurotrema thailandica, Poaceicola arundinis, Polyporus mangshanensis, Pseudocoleophoma typhicola, Pseudodictyosporium thailandica, Pseudophaeosphaeria rubi, Purpureocillium sodanum, Ramariopsis atlantica, Rhodocybe griseoaurantia, Rh. indica, Rh. luteobrunnea, Russula indoalba, Ru. pseudoamoenicolor, Sporidesmium aquaticivaginatum, Sp. olivaceoconidium, Sp. pyriformatum, Stagonospora forlicesenensis, Stagonosporopsis centaureae, Terriera thailandica, Tremateia arundicola, Tr. guiyangensis, Trichomerium bambusae, Tubeufia hyalospora, Tu. roseohelicospora and Wojnowicia italica. New combinations are given for Hermatomyces mirum and Pallidocercospora thailandica. A neotype is proposed for Cortinarius fulvescens. Reference specimens are given for Aquaphila albicans, Leptospora rubella, Platychora ulmi and Meliola pseudosasae, while new host or distribution records are provided for Diaporthe eres, Di. siamensis, Di. foeniculina, Dothiorella iranica, Do. sarmentorum, Do. vidmadera, Helvella tinta and Vaginatispora fuckelii, with full taxonomic details. An asexual state is also reported for the first time in Neoacanthostigma septoconstrictum. This paper contributes to a more comprehensive update and improved identification of many ascomycetes and basiodiomycetes.
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A new species of Thecotheus from Croatia with a key to the known species with apicuate spores
Article
  • Sep 2015
  • SYDOWIA
During systematic research on fimicolous fungi in the Sjeverni Velebit National Park, a new species was revealed. It is described here as Thecotheus platyapiculatus. Taxonomic characters are studied in detail on both living and dead material. Ascospore germination was tested on dung agar and dung oatmeal agar. The most important differential characters of the new species related to apiculi, spore symmetry and ascal strangulation are discussed and compared to the most similar T. keithii. Semi-resistant vacuolar bodies are described as new type of cell inclusions. Guidelines to the standardised procedure of treating Thecotheus collections in order to efficiently characterise and differentiate the species are recommended. The ecology of T. platyapiculatus as well as the importance of traditional way of stock breeding for preserving the fimicolous species is discussed. A worldwide identification key to the apiculate species in the genus Thecotheus is provided. A list of 19 additional fimicolous species found on the horse dung during this study is also given. All ascomycetous species but Peziza vesiculosa are reported as new to Croatian mycobiota.
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Phylogenetic Analysis of Two Ribosomal Dna Regions Indicates Multiple Independent Losses of a Sexual Talaromyces State Among Asexual Penicillium Species in Subgenus Biverticillium
Article
  • Jul 1993
Nucleotide sequences were obtained from the mitochondrial small subunit ribosomal DNA, and the nuclear ribosomal DNA region containing the internal transcribed spacers and 5.8S ribosomal RNA gene for 10 Talaromyces species and six Penicillium species in subgenus Biverticillium. Phylogenetic analysis of the Talaromyces species shows that the majority of the species examined formed a major Talaromyces clade, but that sections and series proposed for these species may not reflect their phylogenetic relationships. Incorporating the strictly mitotic Penicillium species with the meiotic Talaromyces in the phylogenetic analysis demonstrated that the majority of mitotic species have meiotic species as their closest relatives. Thus, multiple independent losses of a meiotic Talaromyces state have occurred among Penicillium species in subgenus Biverticillium. Examination of base substitution patterns for each ribosomal DNA region indicated that the mitochondrial small subunit ribosomal DNA is adenine-thymine rich and biased towards adenine-guanine transitions. The internal transcribed spacers and 5.8S ribosomal RNA gene is guanine-cytosine rich and biased towards cytosine-thymine and thymine-cytosine base transitions. Although these molecules vary in their tempo and mode of evolution, trees generated independently for each ribosomal DNA region from bootstrap analysis had identical topologies in all statistically significant branches.
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Phylogenetic relationships among species of Phillipsia inferred from molecular and morphological data
Article
  • Mar 1999
The internal transcribed spacers (ITS) of the nuclear ribosomal DNA have been sequenced from 29 collections of Phillipsia, mainly from the New World. The P. domingensis complex, collections with a range of colors but otherwise referable to P. domingensis s.l. based on spore ornamentation, were studied. Three distinctive species of Phillipsia also were included. The sequences were analysed to infer phylogenetic relationships within Phillipsia, using parsimony. Morphological features were studied separately, and then evaluated in the context of the ITS phylogeny. Four distinct rDNA lineages, supported by ascospore ornamentation, were identified: the P. crispata, the P. domingensis, the P. olivacea and the P. carnicolor lineages. SEM photographs of the ascospores are presented. Phillipsia lutea and another yellow form were nested within the P. domingensis complex, of those with reddish hymenial colors. Color has been emphasized in taxonomy of Phillipsia, but these results suggest that individuals with strikingly different coloration may be closely related. Levels of ITS sequence divergence in the P. domingensis lineage were low. Based on these data, and morphology as studied thus far, there is no justification for recognizing segregate species within the P. domingensis complex. The Old World collections of the P. domingensis complex were nested within the New World collections, which implies that the P. domingensis lineage is geographically widespread. Phillipsia rugospora is placed in synonymy with P. olivacea and a detailed description of this taxon is given. A lectotype is designated for P. olivacea.
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Coprophilous Fungi of Zulia State, Venezuela. Classes: Plectomycetes and Discomycetes. Division Ascomycota
Article
  • Jul 2001
During a study of coprophilous fungi in 1999-2000 in 17 municipalities of Zulia state, Venezuela, were collected 250 animal dungs samples to determine the apperance of coprophilus fungi. The sample dung was proceed of domestic nd wild animals. The samples were maintained in moist chamber (filter paper in Petri dish with a lid) during 7-18 days at room temperature (24-28 degreesC). Upon examination under the light microscopic, the macroscopic and microscopics characters of the fungus that grew in moist chamber were identified two classes, teen (10) genera and seventeen (17) species, of the Plectomycetes and Discomycetes classes, Division Ascomycota such as: Ascobolus furfuraceus, A. inmersus, y lodophanus carneus. The new records of Venezuela were: Gymnoascus reesli, G. californiensis, Coniochaetidium zulianensis, this is a new record in the world, Copromyces bisporus, Coprotiella venezuelensis, this is a new record in the world, Gymnascella aurantiaca, Kernia pachypleura, Ascobolus stictoideus, Saccobolus citrinus, S. Glaver, S. minimus, S. thaxteri, Peziza fimeti y P. bovina.
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Pezizales
Article
  • Jan 1994
Coming back to Paris was an emotional experience for me, because it was 45 years ago as a graduate student I came on a pilgrimage to meet one of the most influential of people who have ever worked with discomycetes, Mme Marcelle LeGal. She greeted me and treated me with the kind of courtesy and interest that I hope all of us can express towards those who come green and wanting to learn. It was an experience I will never forget, and one which led me in future years to propose a genus Galiella in her honour, and to copropose Marcellina also in her honour, and on whose back many of us stand today. The other great figure of that period on whose contributions much of our classification lies is Jan A. Nannfeldt, another mycologist I met that same year in Uppsala where I went to meet and learn from him.
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