Flotation restricted environmental stimulation technique (R.E.S.T.) involves compromising senses of sound, sight, and touch by creating a quiet, dark environment. The individual lies supine in a tank of Epsom salt and water heated to roughly skin temperature (34-35oC). This study was performed to determine if a one hour flotation R.E.S.T. session would aid in the recovery process following maximal eccentric knee extensions and flexions. Twenty-four untrained male students (23.29 ± 2.1 yr, 184.17 ± 6.85 cm, 85.16 ± 11.54 kg) participated in a randomized repeated measures cross-over study. The participants completed two exercise and recovery protocols: a one hour flotation R.E.S.T. session and a one hour seated control (passive recovery). After isometric muscle strength testing, participants were fatigued with eccentric isokinetic muscle contractions (50 repetitions at 60o/sec) of the non-dominant knee extensors and flexors. Blood lactate, blood glucose, heart rate, OMNI-rating of perceived exertion (OMNI-RPE), perceived pain, muscle soreness and isometric strength were collected before exercise, post-treatment, and 24 and 48 hours later. A multivariate analysis of covariance (MANCOVA) found that treatment had a significant main effect on blood lactate, while subsequent univariate ANOVAs found statistical significance with the immediate post treatment blood lactate measures. The results indicate flotation R.E.S.T. appears to have a significant impact on blood lactate and perceived pain compared to a one hour passive recovery session in untrained healthy males. No difference was found between conditions for muscle strength, blood glucose, muscle soreness, heart rate, or OMNI-RPE. Flotation R.E.S.T. may be utilized for recreational and professional athletes to help reduce blood lactate levels after eccentric exercise.