Differential DNA methylation patterns between high and low responders to a weight loss intervention in overweight or obese adolescents: The EVASYON study

*Department of Nutrition, Food Science, Physiology, and Toxicology, University of Navarra, Pamplona, Spain
The FASEB Journal (Impact Factor: 5.04). 03/2013; 27(6). DOI: 10.1096/fj.12-215566
Source: PubMed


In recent years, epigenetic markers emerged as a new tool to understand the influence of lifestyle factors on obesity phenotypes. Adolescence is considered an important epigenetic window over a human's lifetime. The objective of this work was to explore baseline changes in DNA methylation that could be associated with a better weight loss response after a multidisciplinary intervention program in Spanish obese or overweight adolescents. Overweight or obese adolescents (n=107) undergoing 10 wk of a multidisciplinary intervention for weight loss were assigned as high or low responders to the treatment. A methylation microarray was performed to search for baseline epigenetic differences between the 2 groups (12 subjects/group), and MALDI-TOF mass spectrometry was used to validate (n=107) relevant CpG sites and surrounding regions. After validation, 5 regions located in or near AQP9, DUSP22, HIPK3, TNNT1, and TNNI3 genes showed differential methylation levels between high and low responders to the multidisciplinary weight loss intervention. Moreover, a calculated methylation score was significantly associated with changes in weight, BMI-SDS, and body fat mass loss after the treatment. In summary, we have identified 5 DNA regions that are differentially methylated depending on weight loss response. These methylation changes may help to better understand the weight loss response in obese adolescents.-Moleres, A., Campión, J., Milagro, F. I., Marcos, A., Campoy, C., Garagorri, J. M., Gómez-Martínez, S., Martínez, J. A., Azcona-Sanjulián, M. C., Martí, A. Differential DNA methylation patterns between high and low responders to a weight loss intervention in overweight or obese adolescents: the EVASYON study.

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Available from: Amelia Marti, Jan 08, 2015
    • "Results Results showed that the methylation profile of promoters was significantly altered by dietary supplementation in a gene-and tissue-specific manner, whereas onlytranscription and influences splicing[2,3]. The association between genetics and obesity has been demonstrated in many animal studies, and a relationship has been found between the methylation status of CpG sites located on specific genes and obesity456. Although most studies focus on early life stages, when epigenetic programming is well known to occur789, research is also being put into the effect of diet in adult animal models, where gene– nutrient interaction might also modify methylation profile in an epigenetic and reversible fashion, particularly during " dietary transitions " such as situations of caloric restriction or excess of energy intake in adulthood[10]. "
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