Functional inhibition of UQCRB suppresses angiogenesis in zebrafish

Chemical Genomics National Research Laboratory, Department of Biotechnology, Translational Research Center for Protein Function Control, College of Life Science & Biotechnology, Yonsei University, Seoul 120-749, Republic of Korea.
Biochemical and Biophysical Research Communications (Impact Factor: 2.3). 02/2013; 433(4). DOI: 10.1016/j.bbrc.2013.02.082
Source: PubMed


As a subunit of mitochondrial complex III, UQCRB plays an important role in complex III stability, electron transport, and cellular oxygen sensing. Herein, we report UQCRB function regarding angiogenesis in vivo with the zebrafish (Danio rerio). UQCRB knockdown inhibited angiogenesis in zebrafish leading to the suppression of VEGF expression. Moreover, the UQCRB-targeting small molecule terpestacin also inhibited angiogenesis and VEGF levels in zebrafish, supporting the role of UQCRB in angiogenesis. Collectively, UQCRB loss of function by either genetic and pharmacological means inhibited angiogenesis, indicating that UQCRB plays a key role in this process and can be a prognostic marker of angiogenesis- and mitochondria-related diseases.

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Available from: Ho Jeong Kwon
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    • "Recently, UQCRB, one of the subunits of complex III, was described as an oxygen sensor in hypoxia-induced [6] and VEGFinduced [7] angiogenesis. In addition, functional inhibition of UQCRB by gene knock-down inhibited angiogenesis in zebrafish [19]. Yet, the biological role of UQCRB is still elusive in respect with its pathological effects. "
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