Rapid detection of Salmonella enterica serovar Typhi from Humans

ArticleinJournal of Pure and Applied Microbiology 04(02):837-841 · May 2010with38 Reads
Impact Factor: 0.07 ·

    Abstract

    Present study was carried out to investigate Salmonella enterica serovar Typhi associated with Typhoid fever in humans. A total of six blood samples were collected from six patients (4 males and 2 females) and processed for the isolation and identification of the causative agents and their virulence determinants. Microbial investigation revealed the causative agents Salmonella enterica Serovar Typhi in all the six clinical cases. In polymerase chain reaction (PCR) assay, all the six isolates were found positive for the Invasion gene (invA; 244bp fragment), Tyvelose epimerase gene (tyv; 615bp fragment), phage-1 flagellin gene for d-antigen (fliC-d; 750bp fragment) and Vi antigen genes (viaB; 439bp fragment). This study confirms the association of virulent strains of Salmonella enterica Typhi in occurrence of the Typhoid fever in humans. Present study suggested that PCR can be a useful, high throughput diagnostic tool for the rapid detection of Salmonella enterica Serovar Typhi.