Phytate ( myo -Inositol Hexaphosphate) and Risk Factors for Osteoporosis

Servicio de Prevención de Riesgos Laborales, Gestión Sanitaria de Mallorca, Palma de Mallorca, Spain.
Journal of medicinal food (Impact Factor: 1.63). 01/2009; 11(4):747-52. DOI: 10.1089/jmf.2008.0087
Source: PubMed


Several risk factors seem to play a role in the development of osteoporosis. Phytate is a naturally occurring compound that is ingested in significant amounts by those with diets rich in whole grains. The aim of this study was to evaluate phytate consumption as a risk factor in osteoporosis. In a first group of 1,473 volunteer subjects, bone mineral density was determined by means of dual radiological absorptiometry in the calcaneus. In a second group of 433 subjects (used for validation of results obtained for the first group), bone mineral density was determined in the lumbar column and the neck of the femur. Subjects were individually interviewed about selected osteoporosis risk factors. Dietary information related to phytate consumption was acquired by questionnaires conducted on two different occasions, the second between 2 and 3 months after performing the first one. One-way analysis of variance or Student's t test was used to determine statistical differences between groups. Bone mineral density increased with increasing phytate consumption. Multivariate linear regression analysis indicated that body weight and low phytate consumption were the risk factors with greatest influence on bone mineral density. Phytate consumption had a protective effect against osteoporosis, suggesting that low phytate consumption should be considered an osteoporosis risk factor.

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Available from: Angel Arturo López-González, Dec 19, 2013
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    • "). Acid phosphatase (orthophosphoric monoester phosphohydrolase, EC. and phytase (myo-inositol hexakisphosphate 6-phosphohydrolases; EC hydrolyze phytate and reduce its antinutritional properties (Iqbal et al. 1994; López-González et al. 2008; Palacios et al. 2005). The specificity of acid phosphatase and phytase can partially overlapped since acid phosphatase produced by microorganisms has phytase activity (Simon and Igbasan 2002). "
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    ABSTRACT: The effect of metal ions on the enzymatic activity of Lactobacillus reuteri was studied. The enzymatic activity was determined spectrophotometrically using the corresponding substrate. In the control group, L. reuteri MF14-C, MM2-3, SD2112, and DSM20016 produced the highest α-glucosidase (40.06 ± 2.80 Glu U/mL), β-glucosidase (17.82 ± 1.45 Glu U/mL), acid phosphatase (20.55 ± 0.74 Ph U/mL), and phytase (0.90 ± 0.05 Ph U/mL) respectively. The addition of Mg(2+) and Mn(2+) led to enhance α-glucosidase produced by L. reuteri MM2-3 by 113.6% and 100.6% respectively. α-Glucosidase produced by MF14-C and CF2-7F was decrease in the presence of K(+) by 65.8 and 69.4% respectively. β-Glucosidase activity of MM7 and SD2112 increased in the presence of Ca(2+) (by 121.8 and 129.8%) and Fe(2+) (by 143.9 and 126.7%) respectively. Acid phosphatase produced by L. reuteri CF2-7F and MM2-3 was enhanced in the presence of Mg(2+), Ca(2+) or Mn(2+) by (94.7, 43.2, and 70.1%) and (63.1, 67.8, and 45.6%) respectively. On the other hand, Fe(2+), K(+), and Na(+) caused only slight increase or decrease in acid phosphatase activity. Phytase produced by L. reuteri MM2-3 was increase in the presence of Mg(2+) and Mn(2+) by 51.0 and 74.5% respectively. Ca(2+) enhanced phytase activity of MM2-3 and DSM20016 by 27.5 and 28.9% respectively. The addition of Na(+) or Fe(2+) decreased phytase activity of L. reuteri. On average, Mg(2+) and Mn(2+) followed by Ca(2+) led to the highest enhancement of the tested enzymes. However, the effect of each metal ion on the enzymatic activity of L. reuteri was found to be a strain dependent. Therefore, a maximized level of a target enzyme could be achieved by selecting a combination of specific strain and specific metal ion.
    Full-text · Article · Sep 2013 · SpringerPlus
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    • "In addition, IP6 inhibits mature human primary osteoclasts function, although we did not find such effect on committed RAW 264.7 cells. Therefore, the present investigation demonstrates that IP6 may represent a novel type of selective inhibitor of osteoclasts and prove useful for the treatment of osteoporosis, which agrees with the inhibition of bone resorption by IP6 found in previous in vivo [28] and clinical reports [29], [30]. "
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    ABSTRACT: Inoxitol hexakisphosphate (IP6) has been found to have an important role in biomineralization and a direct effect inhibiting mineralization of osteoblasts in vitro without impairing extracellular matrix production and expression of alkaline phosphatase. IP6 has been proposed to exhibit similar effects to those of bisphosphonates on bone resorption, however, its direct effect on osteoclasts (OCL) is presently unknown. The aim of the present study was to investigate the effect of IP6 on the RAW 264.7 monocyte/macrophage mouse cell line and on human primary osteoclasts. On one hand, we show that IP6 decreases the osteoclastogenesis in RAW 264.7 cells induced by RANKL, without affecting cell proliferation or cell viability. The number of TRAP positive cells and mRNA levels of osteoclast markers such as TRAP, calcitonin receptor, cathepsin K and MMP-9 was decreased by IP6 on RANKL-treated cells. On the contrary, when giving IP6 to mature osteoclasts after RANKL treatment, a significant increase of bone resorption activity and TRAP mRNA levels was found. On the other hand, we show that 1 µM of IP6 inhibits osteoclastogenesis of human peripheral blood mononuclear cells (PBMNC) and their resorption activity both, when given to undifferentiated and to mature osteoclasts. Our results demonstrate that IP6 inhibits osteoclastogenesis on human PBMNC and on the RAW264.7 cell line. Thus, IP6 may represent a novel type of selective inhibitor of osteoclasts and prove useful for the treatment of osteoporosis.
    Full-text · Article · Aug 2012 · PLoS ONE
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    • "Nevertheless, phytates intake has shown beneficial properties, for instance, blood glucose levels, serum total cholesterol and LDL cholesterol are significantly reduced after their intake (Lee et al. 2006; Lee et al. 2007). Furthermore, their consumption demonstrated to increase bone mineral density, thus, preventing osteopororis (Lopez-Gonzalez et al. 2008). Phytates also inhibit the crystallisation of calcium salts (oxalates and phosphates) avoiding the formation of kidney stones (Grases et al. 2000). "

    Full-text · Chapter · Sep 2011
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