Article

Vaccination against Lyme disease: past, present, and future

Division of Bacteriology and Parasitology, Tulane National Primate Research Center Covington, LA, USA.
Frontiers in Cellular and Infection Microbiology (Impact Factor: 3.72). 02/2013; 3:6. DOI: 10.3389/fcimb.2013.00006
Source: PubMed

ABSTRACT

Lyme borreliosis is a zoonotic disease caused by Borrelia burgdorferi sensu lato bacteria transmitted to humans and domestic animals by the bite of an Ixodes spp. tick (deer tick). Despite improvements in diagnostic tests and public awareness of Lyme disease, the reported cases have increased over the past decade to approximately 30,000 per year. Limitations and failed public acceptance of a human vaccine, comprised of the outer surface A (OspA) lipoprotein of B. burgdorferi, led to its demise, yet current research has opened doors to new strategies for protection against Lyme disease. In this review we discuss the enzootic cycle of B. burgdorferi, and the unique opportunities it poses to block infection or transmission at different levels. We present the correlates of protection for this infectious disease, the pros and cons of past vaccination strategies, and new paradigms for future vaccine design that would include elements of both the vector and the pathogen.

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Available from: Monica E Embers, Sep 18, 2014
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    • "Because of their surface localization, bacterial outer membrane proteins (OMPs) can play dual roles as virulence factors and as immune targets (Huntley et al., 2008;Confer and Ayalew, 2013;Embers and Narasimhan, 2013;Pore and Chakrabarti, 2013;Cash, 2014;Christodoulides, 2014). For these reasons, many investigators have attempted large-scale proteomic efforts to identify F. tularensis OMPs, including the use of biotinylation (Chandler et al., 2015), detergents (Twine et al., 2005b;Dresler et al., 2011), or sodium carbonate (Pavkova et al., 2005Pavkova et al., , 2006Janovská et al., 2007) to extract and purify surface proteins. "
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