Comparison of Biochemical and Molecular Tests for Detecting Insecticide Resistance Due to Insensitive Acetylcholinesterase in Culex quinquefasciatus 1

Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, 3150 Rampart Road, Fort Collins, CO 80521, USA.
Journal of the American Mosquito Control Association (Impact Factor: 0.95). 12/2012; 28(4):323-6. DOI: 10.2987/12-6280R.1
Source: PubMed


Insecticide resistance to organophosphates and carbamates can be the result of changes in acetylcholinesterase activity conferred by the ACE-1 mutation. Detection of this altered target site mutation is important in guiding informed decisions for resistance management. In this study we compared a competitive enzyme assay with a polymerase chain reaction assay utilizing a restriction enzyme. Both assays detected the ACE-1 mutation in Culex quinquefasciatus and agreement was 100%. The costs and benefits of each assay are presented.

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Available from: Janet Mcallister, Nov 05, 2014
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