Use of rapid human respiratory syncytial virus
strip tests for detection of bovine respiratory
syncytial virus in experimentally vaccinated
W. Socha, J. Rola
Department of Virology, National Veterinary Research Institute,
Al. Partyzantów 57, 24-100 Puławy, Poland
Three different rapid strip tests: TRU RSV, BinaxNOW RSV and RSV Respi-strip were com-
pared with RT-PCR and ELISA BRSV Ag for the ability to detect bovine respiratory syncytial virus
(BRSV) in nasal swabs collected from calves experimentally vaccinated with live vaccine Rispoval
RS-PI3. The reference strains of BRSV (375 and A51908) were detected by ELISA BRSV Ag
whereas the strains of human respiratory syncytial virus (HRSV) and bovine parainfluenza virus type
3 (BPIV-3) were not. All rapid strip tests as well as RT-PCR reacted positively both to HRSV and
BRSV reference strains and negatively to BPIV-3. The detection limit for RT-PCR was 39.1 TCID50
(strain 375 of BRSV), whereas for each of the rapid tests it was approximately 156 TCID50and 312
TCID50 for antigen ELISA. Diagnostic sensitivity in detecting BRSV in nasal swabs for TRU RSV
and RSV Respi-strip tests was 33% and 50% for BinaxNOW RSV. Diagnostic specificity of TRU
RSV was 100%, whereas for both BinaxNOW and Respi-strip it was 87%. We concluded that TRU
RSV could be used as a supportive rapid test for BRSV screening in nasal swabs taken directly on
a farm. However, due to the small group of animals used in the experiment, the results should be
regarded as preliminary and the study should be repeated on a larger number of animals.
Key words: BRSV, diagnosis, strip tests
Bovine respiratory syncytial virus (BRSV) is an
enveloped, negative-stranded RNA virus belonging to
the Pneumovirus genus of the Paramyxoviridae family.
It is one of the major viral pathogens responsible for
respiratory tract diseases in cattle worldwide. Infec-
tion with BRSV can affect cattle of all ages and
breeds and is characterized by increased respiratory
rate, nasal discharge, fever and cough. The morbidity
Correspondence to: J. Rola, e-mail: email@example.com, tel.: +48 81 889 30 69
is high (60 to 80%), whereas the mortality rate seldom
exceeds 5-10%, although occasionally in some out-
breaks it can reach up to 20% (Valarcher and Taylor
2007). Clinical signs observed during acute disease
may raise the suspicion of BRSV infection but to
make a definitive diagnosis laboratory confirmation is
needed. The most widely used diagnostic methods for
direct detection of BRSV in field specimens are the
virus isolationtest, antigen
munosorbent assay (ELISA) and reverse transcriptase
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