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Molecular characteristics of Human Endogenous
Retrovirus type-W in schizophrenia and bipolar
disorder.
Herv´e Perron, Nora Hamdani, Rapha¨el Faucard, Mohamed Lajnef, St´ephane
Jamain, Claire Daban-Huard, Samuel Sarrazin, Emmanuel Leguen, Josselin
Houenou, Marine Delavest, et al.
To cite this version:
Herv´e Perron, Nora Hamdani, Rapha¨el Faucard, Mohamed Lajnef, St´ephane Jamain, et
al.. Molecular characteristics of Human Endogenous Retrovirus type-W in schizophrenia and
bipolar disorder.. Transl Psychiatry, 2012, 2, pp.e201. <10.1038/tp.2012.125>.<inserm-
00807038>
HAL Id: inserm-00807038
http://www.hal.inserm.fr/inserm-00807038
Submitted on 2 Apr 2013
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Supplementary Information, Perron H. et al. Molecular Psychiatry, 2012.
Page 1 of 17
SUPPLEMENTARY INFORMATION
Molecular characteristics of Human Endogenous
Retrovirus type-W (HERV-W) in schizophrenia and
bipolar disorder.
TABLE OF CONTENT:
I. Complementary Statistical analyses P. 2
TABLE S1: HERV-W RNA RELATIVE EXPRESSION OF THE MSRV SUBTYPE, ALL CONTROLS
TABLE S2: RELATIVE EXPRESSION OF THE MSRV SUBTYPE,
CONTROLS WITH NEGATIVE CRP (C-)
TABLE S3: HERV-W RNA RELATIVE EXPRESSION AND DNA COPY NUMBERS
STATISTICAL ANALYSES EXCLUDING BIPOLAR DISORDER PATIENTS
TREATED WITH VALPROATE.
II. Sequence analyses P. 3
FIGURE S1: SEQUENCE ALIGNEMENTS WITH THE PROBE P3-14
FIGURE S1A: HC RNA P3-4
FIGURE S1B: HC DNA P5-6
FIGURE S1C: BD RNA P7-8
FIGURE S1D: BD DNA P9-10
FIGURE S1E: SZ RNA P11-12
FIGURE S1F: SZ DNA P13-14
FIGURE S2: PHYLOGENETIC TREE REPRESENTATION OF ALIGNED CLONES WITH RELATED P15-17
HERV-W AND DISTANT HERV-K ENV GENE SEQUENCES:
FIGURE S2A: RNA AND DNA CLONES FROM BD P15
FIGURE S2B: RNA AND DNA CLONES FROM SZ P16
FIGURE S2C: RNA AND DNA CLONES FROM HC P17
Supplementary Information, Perron H. et al. Molecular Psychiatry, 2012.
Page 2 of 17
I. Complementary Statistical analyses:
Table S1: HERV-W RNA relative expression of the MSRV subtype, all controls
HERV_W RT
Table S2: C
Table S2: HERV-W relative expression of the MSRV subtype,
Controls with negative CRP (C-)
p BP vs SZ
p SZ vs HC
Table S3: HERV_W RNA relative expression and DNA copy numbers of the MSRV subtype.
0.01
0.007
Statistical analyses excluding Bipolar Disorder Patients treated with Valproate.
0.575
0.0006
Variables BP,N=91 SZ,N=45 Controls,N=73 P for all P BP vs TEM P BP vs SZ P SZ vs TEM
RNA 1.62 (4.6) 0.84 (0.5) 0.65 (0.6) <0.0001 <0.0001 0.01 0.012
DNA 0.61(0.3) 0.55(0.3) 0.77(0.3) 0.0005 0.0016 0.575 0.0003
Mean(SD)
P value Kruskall Wallis/Mann Whitney
Variables BP,N=91 SZ,N=45 Controls,N=46 P for all P BP vs TEM P BP vs SZ P SZ vs TEM
RNA 1.62 (4.6) 0.84 (0.5) 0.62 (0.6) <0.0001 <0.0001 0.01 0.007
DNA 0.60(0.3) 0.55(0.3) 0.77(0.3) 0.0015 0.003 0.575 0.0006
Mean(SD)
P value Kruskall Wallis/Mann Whitney
Variables BP,N=68 SZ,N=45 Controls,N=73 P for all P BP vs TEM P BP vs SZ P SZ vs TEM
RNA 1.71 (5.3) 0.84 (0.5) 0.65 (0.6) <0.0001 <0.0001 0.087 0.007
DNA 0.61(0.3) 0.55(0.3) 0.77(0.3) 0.0009 0.006 0.468 0.0003
Mean(SD)
P value Kruskall Wallis/Mann Whitney
Supplementary Information, Perron H. et al. Molecular Psychiatry, 2012.
Page 3 of 17
II. Sequence analyses
Figure S1: Sequence alignements with the HERV-W/MSRV-env specific Probe
Figure S1A: HC RNA
Supplementary Information, Perron H. et al. Molecular Psychiatry, 2012.
Page 4 of 17
Supplementary Information, Perron H. et al. Molecular Psychiatry, 2012.
Page 5 of 17
Figure S1B: HC DNA
Supplementary Information, Perron H. et al. Molecular Psychiatry, 2012.
Page 6 of 17
Supplementary Information, Perron H. et al. Molecular Psychiatry, 2012.
Page 7 of 17
Figure S1C: BD RNA
Supplementary Information, Perron H. et al. Molecular Psychiatry, 2012.
Page 8 of 17
Supplementary Information, Perron H. et al. Molecular Psychiatry, 2012.
Page 9 of 17
Figure S1D: BD DNA
Supplementary Information, Perron H. et al. Molecular Psychiatry, 2012.
Page 10 of 17
Supplementary Information, Perron H. et al. Molecular Psychiatry, 2012.
Page 11 of 17
Figure S1E: SZ RNA
Supplementary Information, Perron H. et al. Molecular Psychiatry, 2012.
Page 12 of 17
Supplementary Information, Perron H. et al. Molecular Psychiatry, 2012.
Page 13 of 17
Figure S1F: SZ DNA
Supplementary Information, Perron H. et al. Molecular Psychiatry, 2012.
Page 14 of 17
Supplementary Information, Perron H. et al. Molecular Psychiatry, 2012.
Page 15 of 17
Figure S2: Phylogenetic tree representation of aligned clones with related HERV-W and distant
HERV-K env gene sequences:
Figure S2A: RNA and DNA clones from BD
Clones best aligned
with HERV-W ref.
Sequences
Supplementary Information, Perron H. et al. Molecular Psychiatry, 2012.
Page 16 of 17
Figure S2B: RNA and DNA clones from SZ
Clones best aligned
with HERV-W ref.
Sequences
Supplementary Information, Perron H. et al. Molecular Psychiatry, 2012.
Page 17 of 17
Figure S2C: RNA and DNA clones from HC
Clones best aligned
with HERV-W ref.
Sequences