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Molecular characteristics of Human Endogenous
Retrovirus type-W in schizophrenia and bipolar
disorder.
Herv´e Perron, Nora Hamdani, Rapha¨el Faucard, Mohamed Lajnef, St´ephane
Jamain, Claire Daban-Huard, Samuel Sarrazin, Emmanuel Leguen, Josselin
Houenou, Marine Delavest, et al.
To cite this version:
Herv´e Perron, Nora Hamdani, Rapha¨el Faucard, Mohamed Lajnef, St´ephane Jamain, et
al.. Molecular characteristics of Human Endogenous Retrovirus type-W in schizophrenia and
bipolar disorder.. Transl Psychiatry, 2012, 2, pp.e201. <10.1038/tp.2012.125>.<inserm-
00807038>
HAL Id: inserm-00807038
http://www.hal.inserm.fr/inserm-00807038
Submitted on 2 Apr 2013
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Supplementary Information, Perron H. et al. Molecular Psychiatry, 2012.
Page 1 of 17
SUPPLEMENTARY INFORMATION
Molecular characteristics of Human Endogenous
Retrovirus type-W (HERV-W) in schizophrenia and
bipolar disorder.
TABLE OF CONTENT:
I. Complementary Statistical analyses P. 2
TABLE S1: HERV-W RNA RELATIVE EXPRESSION OF THE MSRV SUBTYPE, ALL CONTROLS
TABLE S2: RELATIVE EXPRESSION OF THE MSRV SUBTYPE,
CONTROLS WITH NEGATIVE CRP (C-)
TABLE S3: HERV-W RNA RELATIVE EXPRESSION AND DNA COPY NUMBERS
STATISTICAL ANALYSES EXCLUDING BIPOLAR DISORDER PATIENTS
TREATED WITH VALPROATE.
II. Sequence analyses P. 3
FIGURE S1: SEQUENCE ALIGNEMENTS WITH THE PROBE P3-14
FIGURE S1A: HC RNA P3-4
FIGURE S1B: HC DNA P5-6
FIGURE S1C: BD RNA P7-8
FIGURE S1D: BD DNA P9-10
FIGURE S1E: SZ RNA P11-12
FIGURE S1F: SZ DNA P13-14
FIGURE S2: PHYLOGENETIC TREE REPRESENTATION OF ALIGNED CLONES WITH RELATED P15-17
HERV-W AND DISTANT HERV-K ENV GENE SEQUENCES:
FIGURE S2A: RNA AND DNA CLONES FROM BD P15
FIGURE S2B: RNA AND DNA CLONES FROM SZ P16
FIGURE S2C: RNA AND DNA CLONES FROM HC P17
Supplementary Information, Perron H. et al. Molecular Psychiatry, 2012.
Page 2 of 17
I. Complementary Statistical analyses:
Table S1: HERV-W RNA relative expression of the MSRV subtype, all controls
HERV_W RT
Table S2: C
Table S2: HERV-W relative expression of the MSRV subtype,
Controls with negative CRP (C-)
p BP vs SZ
p SZ vs HC
Table S3: HERV_W RNA relative expression and DNA copy numbers of the MSRV subtype.
0.01
0.007
Statistical analyses excluding Bipolar Disorder Patients treated with Valproate.
0.575
0.0006
Variables BP,N=91 SZ,N=45 Controls,N=73 P for all P BP vs TEM P BP vs SZ P SZ vs TEM
RNA 1.62 (4.6) 0.84 (0.5) 0.65 (0.6) <0.0001 <0.0001 0.01 0.012
DNA 0.61(0.3) 0.55(0.3) 0.77(0.3) 0.0005 0.0016 0.575 0.0003
Mean(SD)
P value Kruskall Wallis/Mann Whitney
Variables BP,N=91 SZ,N=45 Controls,N=46 P for all P BP vs TEM P BP vs SZ P SZ vs TEM
RNA 1.62 (4.6) 0.84 (0.5) 0.62 (0.6) <0.0001 <0.0001 0.01 0.007
DNA 0.60(0.3) 0.55(0.3) 0.77(0.3) 0.0015 0.003 0.575 0.0006
Mean(SD)
P value Kruskall Wallis/Mann Whitney
Variables BP,N=68 SZ,N=45 Controls,N=73 P for all P BP vs TEM P BP vs SZ P SZ vs TEM
RNA 1.71 (5.3) 0.84 (0.5) 0.65 (0.6) <0.0001 <0.0001 0.087 0.007
DNA 0.61(0.3) 0.55(0.3) 0.77(0.3) 0.0009 0.006 0.468 0.0003
Mean(SD)
P value Kruskall Wallis/Mann Whitney
Supplementary Information, Perron H. et al. Molecular Psychiatry, 2012.
Page 3 of 17
II. Sequence analyses
Figure S1: Sequence alignements with the HERV-W/MSRV-env specific Probe
Figure S1A: HC RNA
Supplementary Information, Perron H. et al. Molecular Psychiatry, 2012.
Page 4 of 17
Supplementary Information, Perron H. et al. Molecular Psychiatry, 2012.
Page 5 of 17
Figure S1B: HC DNA
Supplementary Information, Perron H. et al. Molecular Psychiatry, 2012.
Page 6 of 17
Supplementary Information, Perron H. et al. Molecular Psychiatry, 2012.
Page 7 of 17
Figure S1C: BD RNA
Supplementary Information, Perron H. et al. Molecular Psychiatry, 2012.
Page 8 of 17
Supplementary Information, Perron H. et al. Molecular Psychiatry, 2012.
Page 9 of 17
Figure S1D: BD DNA
Supplementary Information, Perron H. et al. Molecular Psychiatry, 2012.
Page 10 of 17
Supplementary Information, Perron H. et al. Molecular Psychiatry, 2012.
Page 11 of 17
Figure S1E: SZ RNA
Supplementary Information, Perron H. et al. Molecular Psychiatry, 2012.
Page 12 of 17
Supplementary Information, Perron H. et al. Molecular Psychiatry, 2012.
Page 13 of 17
Figure S1F: SZ DNA
Supplementary Information, Perron H. et al. Molecular Psychiatry, 2012.
Page 14 of 17
Supplementary Information, Perron H. et al. Molecular Psychiatry, 2012.
Page 15 of 17
Figure S2: Phylogenetic tree representation of aligned clones with related HERV-W and distant
HERV-K env gene sequences:
Figure S2A: RNA and DNA clones from BD
Clones best aligned
with HERV-W ref.
Sequences
Supplementary Information, Perron H. et al. Molecular Psychiatry, 2012.
Page 16 of 17
Figure S2B: RNA and DNA clones from SZ
Clones best aligned
with HERV-W ref.
Sequences
Supplementary Information, Perron H. et al. Molecular Psychiatry, 2012.
Page 17 of 17
Figure S2C: RNA and DNA clones from HC
Clones best aligned
with HERV-W ref.
Sequences
Article
Full-text available
Many neurodegenerative diseases are associated with chronic inflammation in the brain and periphery giving rise to a continuous imbalance of immune processes. Next to inflammation markers, activation of transposable elements, including long intrespersed nuclear elements (LINE) elements and endogenous retroviruses (ERVs), has been identified during neurodegenerative disease progression and even correlated with the clinical severity of the disease. ERVs are remnants of viral infections in the human genome acquired during evolution. Upon activation, they produce transcripts and the phylogenetically youngest ones are still able to produce viral-like particles. In addition, ERVs can bind transcription factors and modulate immune response. Being between own and foreign, ERVs are reviewed in the context of viral infections of the central nervous system, in aging and neurodegenerative diseases. Moreover, this review tests the hypothesis that viral infection may be a trigger at the onset of neuroinflammation and that ERVs sustain the inflammatory imbalance by summarizing existing data of neurodegenerative diseases associated with viruses and/or ERVs.
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