Updating and curating metabolic pathways of TB

Colorado State University, Fort Collins, CO, USA. Electronic address: .
Tuberculosis (Edinburgh, Scotland) (Impact Factor: 2.71). 01/2013; 93(1). DOI: 10.1016/
Source: PubMed


The sequencing of complete genomes has accelerated biomedical research by providing information about the overall coding capacity of bacterial chromosomes. The original TB annotation resulted in putative functional assignment of ∼60% of the genes to specific metabolic functions, however, the other 40% of the encoded ORFs where annotated as conserved hypothetical proteins, hypothetical proteins or encoding proteins of unknown function. The TB research community is now at the beginning of the next phases of post-genomics; namely reannotation and functional characterization by targeted experimentation. Arguably, this is the most significant time for basic microbiology in recent history. To foster basic TB research, the Tuberculosis Community Annotation Project (TBCAP) jamboree exercise began the reannotation effort by providing additional information for previous annotations, and refining and substantiating the functional assignment of ORFs and genes within metabolic pathways. The overall goal of the TBCAP 2012 exercise was to gather and compile various data types and use this information with oversight from the scientific community to provide additional information to support the functional annotations of encoding genes. Another objective of this effort was to standardize the publicly accessible Mycobacterium tuberculosis reference sequence and its annotation. The greatest benefit of functional annotation information of genome sequence is that it fuels TB research for drug discovery, diagnostics, vaccine development and epidemiology.

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Available from: Richard A Slayden
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    • "The SOS response, which prevents cell cycle progression in response to DNA damage and other stresses, is a classic example of cell division regulation under conditional stress [7-16]. Although proteins that regulate septum formation have been widely characterized in other bacterial species, proteins that carry out this function have not all been identified or thoroughly characterized in Mtb[17]. A previous study from our laboratory identified a septum site determining protein, Ssd, and showed that it is capable of regulating septum formation and inducing alternative metabolic pathways, demonstrating a relationship between the regulation of cell cycle progression and the induction of molecular programs associated with NRP [6]. "
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