Air-dried leaves of Citrus microcarpa Bunge afforded 3',4',5,6,7,8-hexamethoxyflavone (1) and squalene. The structure of 1 was elucidated by extensive 1D and 2D NMR spectroscopy. Antimicrobial tests on 1 indicated that it has moderate activity against the fungus, C. albicans and low activity against the bacteria, P. aeruginosa and B. subtilis and fungi, T. mentagrophytes and A. niger.