The shade-dried powder of leaves of Plectranthus amboinicus (Lour) Spreng was subjected to successive extraction using the solvents, pet. ether, chloroform, ethanol and water, in the increasing order of polarity. A preliminary phytochemical analysis was carried out for all the extracts. It was found that the leaves revealed the presence of alkaloids, carbohydrates, glycosides, proteins, amino acids, fl avonoids, quinine, tannins, phenolic compounds and terpenoids. Because the phytoconstituents present in the ethanolic and aqueous extracts were similar, both extracts were selected for further study. The hepatoprotective effects of ethanolic and aqueous extracts were evaluated against carbon tetrachloride (CCl 4)-induced hepatotoxicity on Wistar albino rats. CCl 4 signifi cantly (P < 0.01) elevated the serum level of biochemical parameters such as aspartate transaminase, alanine transaminase, alkaline phosphate, gamma-glutamyl transpeptidase and bilirubin (total and direct). Rats treated with the ethanolic extract showed a prominent restoration (P < 0.01) whereas the aqueous extract showed a mild restoration (P < 0.01) of all the parameters. The ethanolic and aqueous extract-treated groups also showed regeneration of hepatocytes, normalization of fatty changes and necrosis. Thus, from the present study, it may concluded that the leaves of Plectranthus amboinicus (Lour) Spreng posses hepatoprotective activities to support its traditional uses.