Isolation of Bartonella species from rodents in Taiwan including a strain closely related to 'Bartonella rochalimae' from Rattus norvegicus

Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung 402, Taiwan.
Journal of Medical Microbiology (Impact Factor: 2.25). 01/2009; 57(Pt 12):1496-501. DOI: 10.1099/jmm.0.2008/004671-0
Source: PubMed


An increasing number of Bartonella species originally isolated from small mammals have been identified as emerging human pathogens. During an investigation of Bartonella infection in rodent populations carried out in Taiwan in 2006, a total of 58 rodents were tested. It was determined that 10.3 % (6/58) of the animals were Bartonella bacteraemic. After PCR/RFLP analysis, four isolates were identified as Bartonella elizabethae and one isolate as Bartonella tribocorum. However, there was one specific isolate with an unrecognized PCR/RFLP pattern. After further sequence and phylogenetic analyses of the gltA, ftsZ and rpoB genes, and the 16S-23S rRNA intergenic spacer region, the results indicated that this specific isolate from Rattus norvegicus was closely related to human pathogenic 'Bartonella rochalimae'. Further studies need to be conducted to evaluate whether this rodent species could be a reservoir for 'B. rochalimae'.

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    • "Arthropods feeding on small mammals are involved in the transmission routes of Bartonella species. Fleas are important vectors for the maintenance and transmission of many Bartonella species among populations of small mammals [6] [7]. "
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    • "vinsonii (Brenner et al. 1993), B. doshiae, B. grahamii, and B. taylorii (Birtles et al. 1995), B. tribocorum (Heller et al. 1998), B. vinsonii subsp. arupensis (Welch et al. 1999), B. birtlesii (Bermond et al. 2000), B. washoensis (Kosoy et al. 2003), B. phoceensis and B. rattimassiliensis (Gundi et al. 2004), B. rochalimae (Lin et al. 2008), B. tamiae (Kosoy et al. 2008), B. rattaustraliani, B. queenslandensis, and B. coopersplainsensis (Gundi et al. 2009), B. japonica and B. silvatica (Inoue et al. 2010), and B. jaculi, B. callosciuri, B. pachyuromydis , and B. acomydis (Sato et al. 2013). Other Bartonella isolated from rodents have been proposed as new species or subspecies, including Candidatus Bartonella washoensis subsp. "
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    • "arupensis (Welch et al., 1999), Bartonella doshiae (Birtles et al., 1995), Bartonella taylorii (Birtles et al., 1995), Bartonella vinsonii subsp. vinsonii (Ellis et al., 1999), Bartonella tribocorum (Heller et al., 1998), Bartonella washoensis (Kosoy et al., 2003), Bartonella rattimassiliensis (Gundi et al., 2004), Bartonella phoceensis (Gundi et al., 2004), Bartonella birtlesii (Bermond et al., 2000), Bartonella rochalimae (Lin et al., 2008), Bartonella japonica (Inoue et al., 2010), and Bartonella silvatica (Inoue et al., 2010). The order Rodentia has been reported as the reservoir for several zoonotic Bartonella species, such as B. elizabethae (Daly et al., 1993), and B. washoensis (Kosoy et al., 2003) and B. grahamii (Kerkhoff et al., 1999). "
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