Article

Two Leucine-Rich Repeat Receptor Kinases Mediate Signaling, Linking Cell Wall Biosynthesis and ACC Synthase in Arabidopsis

Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599-3280, USA.
The Plant Cell (Impact Factor: 9.34). 12/2008; 20(11):3065-79. DOI: 10.1105/tpc.108.063354
Source: PubMed

ABSTRACT

The plant cell wall is a dynamic structure that changes in response to developmental and environmental cues through poorly understood signaling pathways. We identified two Leu-rich repeat receptor-like kinases in Arabidopsis thaliana that play a role in regulating cell wall function. Mutations in these FEI1 and FEI2 genes (named for the Chinese word for fat) disrupt anisotropic expansion and the synthesis of cell wall polymers and act additively with inhibitors or mutations disrupting cellulose biosynthesis. While FEI1 is an active protein kinase, a kinase-inactive version of FEI1 was able to fully complement the fei1 fei2 mutant. The expansion defect in fei1 fei2 roots was suppressed by inhibition of 1-aminocyclopropane-1-carboxylic acid (ACC) synthase, an enzyme that converts Ado-Met to ACC in ethylene biosynthesis, but not by disruption of the ethylene response pathway. Furthermore, the FEI proteins interact directly with ACC synthase. These results suggest that the FEI proteins define a novel signaling pathway that regulates cell wall function, likely via an ACC-mediated signal.

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    • "Three LRR RLKs were also found in the two QTLs associated with BFS and length (Table 5 , Figures 6 and 7). In Arabidopsis elongating root tips and seeds, two LRR RLKs named FEI 1 and 2 have been reported to play a role in cellulose deposition in Arabidopsis elongating root tips [34] and seed coat [35] . ACC (1-aminocyclopropane-1-carbox- ylic acid) that is essential for ethylene signaling has been suggested to be a signaling molecule for FEI 1 and 2 [58], so both ethylene and LRR RLK signaling are most likely involved in cellulose deposition in elongating tissues (Fig. 8). "

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