Huang, H. M. et al. Phosphorylation sites in BubR1 that regulate kinetochore attachment, tension, and mitotic exit. J. Cell Biol. 183, 667-680

Fox Chase Cancer Center, Philadelphia, PA 19111, USA.
The Journal of Cell Biology (Impact Factor: 9.83). 12/2008; 183(4):667-80. DOI: 10.1083/jcb.200805163
Source: PubMed


BubR1 kinase is essential for the mitotic checkpoint and also for kinetochores to establish microtubule attachments. In this study, we report that BubR1 is phosphorylated in mitosis on four residues that differ from sites recently reported to be phosphorylated by Plk1 (Elowe, S., S. Hummer, A. Uldschmid, X. Li, and E.A. Nigg. 2007. Genes Dev. 21:2205-2219; Matsumura, S., F. Toyoshima, and E. Nishida. 2007. J. Biol. Chem. 282:15217-15227). S670, the most conserved residue, is phosphorylated at kinetochores at the onset of mitosis and dephosphorylated before anaphase onset. Unlike the Plk1-dependent S676 phosphorylation, S670 phosphorylation is sensitive to microtubule attachments but not to kinetochore tension. Functionally, phosphorylation of S670 is essential for error correction and for kinetochores with end-on attachments to establish tension. Furthermore, in vitro data suggest that the phosphorylation status of BubR1 is important for checkpoint inhibition of the anaphase-promoting complex/cyclosome. Finally, RNA interference experiments show that Mps1 is a major but not the exclusive kinase that specifies BubR1 phosphorylation in vivo. The combined data suggest that BubR1 may be an effector of multiple kinases that are involved in discrete aspects of kinetochore attachments and checkpoint regulation.

Download full-text


Available from: Tim J Yen, May 06, 2015
  • Source
    • "Aurora kinases also phosphorylate the Zwint-1 protein to recruit RZZ, Spindly, and dynactin although other sites and kinases may be involved (Ditchfield et al. 2003; Kasuboski et al. 2011). Aurora B is an upstream regulator because Mad1 recruitment in metazoans requires both Bub1 and RZZ (Huang et al. 2008; Kops et al. 2005; Williams et al. 2003). Aurora B kinase also phosphorylates the Mis12 complex on Dsn1/Mis13 to recruit KMN, and there is a second pathway to recruit KMN dependent upon CENP-H/I/K/M (Basilico et al. 2014; Kim and Yu 2015; Yang et al. 2008). "
    [Show abstract] [Hide abstract]
    ABSTRACT: Kinetochores generate a signal that inhibits anaphase progression until every kinetochore makes proper attachments to spindle microtubules. This spindle assembly checkpoint (SAC) increases the fidelity of chromosome segregation. We will review the molecular mechanisms by which kinetochores generate the SAC and extinguish the signal after making proper attachments, with the goal of identifying unanswered questions and new research directions. We will emphasize recent breakthroughs in how phosphorylation changes drive the activation and inhibition of the signal. We will also emphasize the dramatic changes in kinetochore structure that occur after attaching to microtubules and how these coordinate SAC function with microtubule attachment status. Finally, we will review the emerging cross talk between the DNA damage response and the SAC.
    Full-text · Article · Apr 2015 · Chromosoma
  • Source
    • "The two groups identified a highly phosphorylated conserved domain of BubR1 (residues 665–682), referred to as KARD (Suijkerbuijk et al., 2012b), whose integrity is necessary for the ability of BubR1 to promote stable K–MT attachments. Within the KARD domain, there are at least three phosphorylation sites: two (S670 and S676) described above (Elowe et al., 2007, 2010; Huang et al., 2008) plus a third site (T680), also phosphorylated by Plk1 on tensionless kinetochores, as is S676 (Suijkerbuijk et al., 2012b). Expressing the triple S670A, S676A, and T680A BubR1 mutant in HeLa cells abolished chromosome alignment, but chemically inhibiting Aurora B activity largely restored proper chromosome alignment in these cells. "
    [Show abstract] [Hide abstract]
    ABSTRACT: BubR1 is a critical component of the spindle assembly checkpoint, the surveillance mechanism that helps maintain the high fidelity of mitotic chromosome segregation by preventing cells from initiating anaphase if one or more kinetochores are not attached to the spindle. BubR1 also helps promote the establishment of stable kinetochore-microtubule attachments during prometaphase. In this chapter, we review the structure, functions, and regulation of BubR1 in these "classical roles" at the kinetochore. We discuss its recruitment to kinetochores, its assembly into the inhibitor of anaphase progression, and the importance of its posttranslational modifications. We also consider the evidence for its participation in other roles beyond mitosis, such as the meiosis-specific processes of recombination and prophase arrest of the first meiotic division, the cellular response to DNA damage, and in the regulation of centrosome and basal body function. Finally, studies are presented linking BubR1 dysfunction or misregulation to aging and human disease, particularly cancer.
    Full-text · Article · Sep 2013 · International review of cell and molecular biology
  • Source
    • "670–720) are required for binding B56-PP2A. BUBR1 is hyperphosphorylated during mitosis, and mitotic kinases Cdk1, Plk1 and Mps1 have been shown to phosphorylate S670 (Elowe et al., 2007; Huang et al., 2008) (in response to lack of kinetochore–microtubule attachment) and S676 (Elowe et al., 2007) (in response to lack of tension) within this evolutionary conserved second motif of BUBR1. During the preparation of this manuscript, Kops and his colleague show that phosphorylation within this second motif by Plk1 promotes interaction of BUBR1 with B56α-PP2A to counter excessive Aurora B activity at the kinetochores (Suijkerbuijk et al., 2012). "
    [Show abstract] [Hide abstract]
    ABSTRACT: BUBR1 is a mitotic phosphoprotein essential for the maintenance of chromosome stability by promoting chromosome congression and proper kinetochore-microtubule (K-fiber) attachment, but the underlying mechanism(s) has remained elusive. Here we identify BUBR1 as a binding partner of the B56 family of Protein Phosphatase 2A regulatory subunits. The interaction between BUBR1 and the B56 family is required for chromosome congression, since point mutations in BUBR1 that block B56 binding abolish chromosome congression. The BUBR1:B56-PP2A complex opposes Aurora B kinase activity, since loss of the complex can be reverted by inhibiting Aurora B. Importantly, we show that the failure of BUBR1 to recruit B56-PP2A also contributes to the chromosome congression defects found in cells derived from patients with the Mosaic Variegated Aneuploidy (MVA) syndrome. Together, we propose that B56-PP2A is a key mediator of BUBR1's role in chromosome congression and functions by antagonizing Aurora B activity at the kinetochore for establishing stable kinetochore-microtubule attachment at the metaphase plate.
    Full-text · Article · May 2013 · Biology Open
Show more