Article

Mask Proteins Are Cofactors of Yorkie/YAP in the Hippo Pathway

Cancer Research UK, London Research Institute, 44 Lincoln's Inn Fields, London WC2A 3LY, UK.
Current biology: CB (Impact Factor: 9.57). 01/2013; 23(3). DOI: 10.1016/j.cub.2012.11.061
Source: PubMed

ABSTRACT

The Hippo signaling pathway acts via the Yorkie (Yki)/Yes-associated protein (YAP) transcriptional coactivator family to control tissue growth in both Drosophila and mammals [1-3]. Yki/YAP drives tissue growth by activating target gene transcription, but how it does so remains unclear. Here we identify Mask as a novel cofactor for Yki/YAP. We show that Drosophila Mask forms a complex with Yki and its binding partner, Scalloped (Sd), on target-gene promoters and is essential for Yki to drive transcription of target genes and tissue growth. Furthermore, the stability and subcellular localization of both Mask and Yki is coregulated in response to various stimuli. Finally, Mask proteins are functionally conserved between Drosophila and humans and are coexpressed with YAP in a wide variety of human stem/progenitor cells and tumors.

Download full-text

Full-text

Available from: Barry J Thompson, Oct 28, 2015
  • Source
    • "The fly stocks used were dac 3 , UAS - dac F ( Mardon et al . , 1994 ) , UAS - HA : dac F ( Tavsanli et al . , 2004 ) , eya E8 ( Bonini et al . , 1998 ) , tkv 4 , hpo 42 – 47 ( Wu et al . , 2003b ) , UAS - hth - GFP ( Casares and Mann , 2000 ) , UAS - yki ( Huang et al . , 2005 ) , UAS - yki : HA ( Sidor et al . , 2013 ) , UAS - yki - IR 4005R - 2 ( NIG ) , UAS - hth - IR ( VDRC #12763 ) , UAS - ex ( Udan et al . , 2003 ) , GFP - ban sensor ( Brennecke et al . , 2003 ) , ban - lacZ ( Spradling et al . , 1999 ) , UAS - ban EP ( 3 ) 3622 ( Rorth et al . , 1998 ) , UAS - ban - sponge ( Becam et al . , 2011 ) , Diap1 : : lacZ ( Hay et al . , 1995 ) , ptc "
    [Show abstract] [Hide abstract]
    ABSTRACT: The Drosophila transcriptional co-activator protein Yorkie and its vertebrate orthologs YAP and TAZ are potent oncogenes, whose activity is normally kept in check by the upstream Hippo kinase module. Upon its translocation into the nucleus, Yorkie forms complexes with several tissue-specific DNA-binding partners, which help to define the tissue-specific target genes of Yorkie. In the progenitor cells of the eye imaginal disc, the DNA-binding transcription factor Homothorax is required for Yorkie-promoted proliferation and survival through regulation of the bantam microRNA (miRNA). The transit from proliferating progenitors to cell cycle quiescent precursors is associated with the progressive loss of Homothorax and gain of Dachshund, a nuclear protein related to the Sno/Ski family of co-repressors. We have identified Dachshund as an inhibitor of Homothorax-Yorkie-mediated cell proliferation. Loss of dachshund induces Yorkie-dependent tissue overgrowth. Conversely, overexpressing dachshund inhibits tissue growth, prevents Yorkie or Homothorax-mediated cell proliferation of disc epithelia and restricts the transcriptional activity of the Yorkie-Homothorax complex on the bantam enhancer in Drosophila cells. In addition, Dachshund collaborates with the Decapentaplegic receptor Thickveins to repress Homothorax and Cyclin B expression in quiescent precursors. The antagonistic roles of Homothorax and Dachshund in Yorkie activity, together with their mutual repression, ensure that progenitor and precursor cells are under distinct proliferation regimes. Based on the crucial role of the human dachshund homolog DACH1 in tumorigenesis, our work suggests that DACH1 might prevent cellular transformation by limiting the oncogenic activity of YAP and/or TAZ. © 2015. Published by The Company of Biologists Ltd.
    Full-text · Article · Mar 2015 · Development
  • Source
    • "The overexpression of ANKHD1 has been reported in acute leukemias [15] and multiple myeloma cells [16], and has been found to be associated with a significantly decreased survival in breast cancer patients [17]. The recent identification of ANKHD1 as a novel member of the Hippo signaling pathway has provided new possibilities for investigation [17] [18]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: ANKHD1 is a multiple ankyrin repeat containing protein, recently identified as a novel member of the Hippo signaling pathway. The present study aimed to investigate the role of ANKHD1 in DU145 and LNCaP prostate cancer cells. ANKHD1 and YAP1 were found to be highly expressed in prostate cancer cells, and ANKHD1 silencing decreased cell growth, delayed cell cycle progression at the S phase, and reduced tumor xenograft growth. Moreover, ANKHD1 knockdown downregulated YAP1 expression and activation, and reduced the expression of CCNA2, a YAP1 target gene. These findings indicate that ANKHD1 is a positive regulator of YAP1 and promotes cell growth and cell cycle progression through Cyclin A upregulation.
    Full-text · Article · Apr 2014 · Experimental Cell Research
  • Source
    • "Activation of the Wts/Mats complex deactivates Yorkie (Huang et al., 2005) (Yki) by phosphorylation . This triggers cytoplasmic retention and inhibits the formation of a complex involving Multiple Ankyrin-repeat Single KH (MASK) proteins and the anti-apoptotic and pro-proliferative transcription factor Scalloped (Sd) (Goulev et al., 2008; Sansores-Garcia et al., 2013; Sidor et al., 2013; Wu et al., 2008; L. Zhang et al., 2008). This Hpo/Sav-Wts/Mats mediated downregulation of Sd transcription is the primary mechanism of tumor suppression and growth control activity of the Hippo pathway (L. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Cells in vivo are exposed to a complex signaling environment. Biochemical signaling modalities, such as secreted proteins, specific extracellular matrix domains and ion fluxes certainly compose an important set of regulatory signals to cells. However, these signals are not exerted in isolation, but rather in concert with biophysical cues of the surrounding tissue, such as stiffness and topography. In this review, we attempt to highlight the biophysical attributes of ocular tissues and their influence on cellular behavior. Additionally, we introduce the proteins YAP and TAZ as targets of biophysical and biochemical signaling and important agonists and antagonists of numerous signaling pathways, including TGFβ and Wnt. We frame the discussion around this extensive signaling crosstalk, which allows YAP and TAZ to act as orchestrating molecules, capable of integrating biophysical and biochemical cues into a broad cellular response. Finally, while we draw on research from various fields to provide a full picture of YAP and TAZ, we attempt to highlight the intersections with vision science and the exciting work that has already been performed.
    Full-text · Article · Jun 2013 · Experimental Eye Research
Show more