Bioluminescent Indicators for Ca2+ Based on Split Renilla Luciferase Complementation in Living Cells

ArticleinAnalytical Sciences 24(11):1405-8 · February 2008with29 Reads
Impact Factor: 1.39 · DOI: 10.2116/analsci.24.1405 · Source: PubMed

    Abstract

    Genetically encoded bioluminescent indicators for intracellular Ca2+ are described here with CaM-M13 interaction-induced complementation of split Renilla luciferase. The Ca2+-induced interaction between CaM and M13 leads to complementation of the N- and C-terminal halves of split Renilla luciferase in living cells. This intramolecular interaction results in the spontaneous and simultaneous emission of bioluminescence split Renilla luciferase. This is how intracellular Ca2+ is illuminated with the intramolecular complementation of split Renilla luciferase. The Ca2+-dependent spontaneous and simultaneous emission of bioluminescence promises to reveal Ca2+ dynamics in living cells, and also in vivo using the present indicators.