Glaciers on Earth along other components of the cryosphere are important for the climate system. However, it is widely known that the vast majority of glaciers are retreating and thinning since the early part of the 20th century. Additionally, future projections have highlighted that at the end of the 21st century, glaciers are going to lose a considerable part of their remaining mass. These glacier changes have several implications for physical, biological and human systems, affecting the water availability for downstream communities and contribute to sea level rise. Unlike other regions, where glaciers are less relevant for the overall hydrology, glaciers in South America constitute a critical resource since minimum flow levels in headwaters of the Andean mountains are usually sustained by ice melt, especially during late summer and droughts, when the contribution from the seasonal snow cover is depleted. In the last decades, the number of studies has increased considerable, however, in the Southern Andes and the surrounding sub-Antarctic islands glaciers still are less studied in comparison with their counterparts in the Northern Hemisphere. The few studies on glacier mass balance in this region suggest a risk of water scarcity for many Andean cities which freshwater supply depends on glacial meltwater. Additionally, glaciers on sub-Antarctic islands have not been completely assessed and their contribution to the sea level rise has been roughly estimated. Hence, the monitoring of glaciers is critical to provide baseline information for regional climate change adaptation policies and facilitate potential hazard assessments. Close and long-range remote sensing techniques offer the potential for repeated measurements of glacier variables (e.g. glacier mass balance, area changes). In the last decades, the number of sensors and methods has increased considerably, allowing time series analysis as well as new and more precise measurements of glacier changes. The main goal of this thesis is to investigate and provide a detailed quantification of glacier elevation and mass changes of the Southern Andes with strong focus on the Central Andes of Chile and South Georgia. Six comprehensive studies were performed to provide a better understanding of the development and current status of glaciers in this region. Overall, the glacier changes were estimated by means of various remote sensing techniques. For the Andes as a whole, the first continent-wide glacier elevation and mass balance was conducted for 85% of the total glacierized area of South America. A detailed estimation of mass changes using the bi-static synthetic aperture radar interferometry (Shuttle Radar Topography Mission -SRTM- and TerraSAR-X add-on for Digital Elevation Measurements -TanDEM-X- DEMs) over the years 2000 to 2011/2015 was computed. A total mass loss rate of 19.43 ± 0.60 Gt a-1 (0.054 ± 0.002 mm a-1 sea level rise contribution) from elevation changes above ground, sea or lake level was calculated, with an extra 3.06 ± 1.24 Gt a-1 derived from subaqueous ice mass loss. The results indicated that about 83% of the total mass loss observed in this study was contributed by the Patagonian icefields (Northern and Southern), which can largely be explained by the dynamic adjustments of large glaciers. For the Central Andes of Chile, four studies were conducted where detailed times series of glacier area, mass and runoff changes were performed on individual glaciers and at a region level (Maipo River basin). Glaciers in the central Andes of Chile are a fundamental natural resources since they provide freshwater for ecosystems and for the densely populated Metropolitan Region of Chile. The first study was conducted in the Maipo River basin to obtain time series of basin-wide glacier mass balance estimates. The estimations were obtained using historical topographic maps, SRTM, TanDEM-X, and airborne Light Detection and Ranging (LiDAR) digital elevation models. The results showed spatially heterogeneous glacier elevation and mass changes between 1955 and 2000, with more negative values between 2000 and 2013. A mean basin-wide glacier mass balance of −0.12 ± 0.06 m w.e. a-1 , with a total mass loss of 2.43 ± 0.26 Gt between 1955–2013 was calculated. For this region, a 20% reduction in glacier ice volume since 1955 was observed with associated consequences for the meltwater contribution to the local river system. Individual glacier studies were performed for the Echaurren Norte and El Morado glaciers. Echaurren Norte Glacier is a reference glacier for the World Glacier Monitoring Service. An ensemble of different data sets was used to derive a complete time series of elevation, mass and area changes. For El Morado Glacier, a continuous thinning and retreat since the 20th century was found. Overall, highly negative elevation and mass changes rates were observed from 2010 onwards. This coincides with the severe drought in Chile in this period. Moreover, the evolution of a proglacial lake was traced. If drained, the water volume poses an important risk to down-valley infrastructure. The glacier mass balance for the Central Andes of Chile has been observed to be highly correlated with precipitation (ENSO). All these changes have provoked a glacier volume reduction of one-fifth between 1955 and 2016 and decrease in the glacier runoff contribution in the Maipo basin. The thesis closes with the first island-wide glacier elevation and mass change study for South Georgia glaciers, one of the largest sub-Antarctic islands. There, glaciers changes were inferred by bi-static synthetic aperture radar interferometry between 2000 and 2013. Frontal area changes were mapped between 2003 and 2016 to roughly estimate the subaqueous mass loss. Special focus was given to Szielasko Glacier where repeated GNSS measurements were available from 2012 and 2017. The results showed an average glacier mass balance of −1.04 ± 0.09 m w.e. a-1 and a mass loss rate of 2.28 ± 0.19 Gt a-1 (equivalent to 0.006 ± 0.001 mm a-1 sea level rise) in the period 2000-2013. An extra 0.77 ± 0.04 Gt a-1 was estimated for subaqueous mass loss. The concurrent area change rate of the marine and lake-terminating glaciers amounts to −6.58 ± 0.33 km2 a-1 (2003–2016). Overall, the highest thinning and retreat rates were observed for the large outlet glaciers located at the north-east coast. Neumayer Glacier showed the highest thinning rates with the disintegration of some tributaries. Our comparison between InSAR data and GNSS measurements showed good agreement, demonstrating consistency in the glacier elevation change rates from two different methods. Our glacier elevation and mass changes assessment provides a baseline for further comparison and calibration of model projection in a sparsely investigated region. Future field measurements, long-term climate reanalysis, and glacier system modelling including ice-dynamic changes are required to understand and identify the key forcing factors of the glacier retreat and thinning.