Vaccinium myrtillus (Bilberry) Extracts Reduce Angiogenesis In Vitro and In Vivo

Article (PDF Available)inEvidence-based Complementary and Alternative Medicine 7(1):47-56 · November 2007with71 Reads
DOI: 10.1093/ecam/nem151 · Source: PubMed
Vaccinium myrtillus (Bilberry) extracts (VME) were tested for effects on angiogenesis in vitro and in vivo. VME (0.3-30 µg ml(-1)) and GM6001 (0.1-100 µM; a matrix metalloproteinase inhibitor) concentration-dependently inhibited both tube formation and migration of human umbilical vein endothelial cells (HUVECs) induced by vascular endothelial growth factor-A (VEGF-A). In addition, VME inhibited VEGF-A-induced proliferation of HUVECs. VME inhibited VEGF-A-induced phosphorylations of extracellular signal-regulated kinase 1/2 (ERK 1/2) and serine/threonine protein kinase family protein kinase B (Akt), but not that of phospholipase Cγ (PLCγ). In an in vivo assay, intravitreal administration of VME inhibited the formation of neovascular tufts during oxygen-induced retinopathy in mice. Thus, VME inhibited angiogenesis both in vitro and in vivo, presumably by inhibiting the phosphorylations of ERK 1/2 and Akt. These findings indicate that VME may be effective against retinal diseases involving angiogenesis, providing it can reach the retina after its administration. Further investigations will be needed to clarify the major angiogenesis-modulating constituent(s) of VME.
6 Figures
    • "Pure anthocyanins and anthocyanin-rich extracts from fruits and vegetables have exhibited anti-proliferative activity towards multiple cell types in vitro (Choi et al., 2011; Matsunaga et al., 2010a; Shih et al., 2005; Tanaka et al., 2012; Wang and Stoner, 2008; Zhang et al., 2005). The non-toxic anthocyanins from purple sweet potato and blueberry used in this study demonstrated potent growth inhibitory properties in RPE cells, the proliferation of which might cause PVR. "
    [Show abstract] [Hide abstract] ABSTRACT: Purple Sweet Potato Anthocyanin (PSPA), a class of naturally occurring anthocyanins derived from purple sweet potato storage roots, possesses unique color and multiple bioactivities. This study investigated the anti-proliferative effect of PSPA in human Retinal Pigment Epithelial (RPE) cells, the proliferation of which accounts for Proliferative Vitreoretinopathy (PVR). Blueberry Anthocyanin (BBA) was used as a contrast. PSPA and BBA inhibited RPE proliferation time-and dose-dependently through blocking the cell cycle in G0/G1 phase and inducing apoptosis via ROS accumulation, DNA damage and caspase 3/7 activation. Meanwhile, PSPA showed stronger potential than BBA in inhibiting RPE growth. Hence, we highlighted the importance of dietary supplementation of anthocyanins in PVR prevention and the application of PSPA in health industry.
    Full-text · Article · Jul 2016 · BMC Complementary and Alternative Medicine
    • "Angiogenesis is the physiological process of forming new blood vessels from the preexisting vasculature, and it is a vital process during embryonic development. However, in adults angiogenesis is only observed in specific areas, such as the endometrium and ovarian follicle cells [1]. Angiogenesis also plays a key role in many diseases, including cancer, where it promotes tumor growth and metastasis [2]. "
    Data · Apr 2015 · BMC Complementary and Alternative Medicine
    • "Angiogenesis is observed during embryonic vascular development , wound healing, and organ regeneration [3]. In adults, angiogenesis is only observed in specific areas, such as the endometrium and ovarian follicle cells [4]. Pathologically, angiogenesis is closely related to diverse diseases, particularly cancer. "
    [Show abstract] [Hide abstract] ABSTRACT: Background Angiogenesis, which is initiated by certain tumor micro-environmental conditions and diverse protein factors, plays a pivotal role during tumor development and metastasis. Therefore, many efforts have been made to develop effective anti-angiogenic agents as anticancer therapeutics. In the current study, we investigated the anti-angiogenic potential of an ethanol extract of Annona atemoya seeds (EEAA) in vitro and in vivo. Methods The anti-angiogenic potential of EEAA was evaluated using various in vitro/in vivo models, including cell proliferation, migration, and tube formation by human umbilical vascular endothelial cells (HUVECs); a Matrigel plug assay; and tumor-induced angiogenesis. The expression of hypoxia-inducible factors (HIFs) and vascular endothelial growth factor (VEGF) was investigated using reverse transcription-polymerase chain reaction, immunoassays, and western blotting. Results EEAA was able to significantly inhibit the angiogenic properties of HUVECs in vitro as well as angiogenic factor-induced blood vessel formation in vivo. EEAA down-regulated the expression of VEGF and HIF-1alpha/2alpha at the mRNA and protein levels, respectively, in cancer cells under hypoxic conditions. Conclusions EEAA shows a strong anti-angiogenic potential in both in vitro and in vivo systems, and we suggest that EEAA may be a valuable herbal source for anticancer drug development.
    Full-text · Article · Sep 2014
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