Intestinal Microbiota Containing Barnesiella Species Cures Vancomycin-Resistant Enterococcus faecium Colonization

Infectious Diseases Service, Department of Medicine.
Infection and immunity (Impact Factor: 3.73). 01/2013; 81(3). DOI: 10.1128/IAI.01197-12
Source: PubMed


Bacteria causing infections in hospitalized patients are increasingly antibiotic-resistant. Classical infection control practices are only partially effective at preventing spread of antibiotic-resistant bacteria within hospitals. Because the density of intestinal colonization by the highly antibiotic-resistant bacterium vancomycin-resistant Enterococcus (VRE) can exceed 10(9) organisms per gram of feces, even optimally implemented hygiene protocols often fail. Decreasing the density of intestinal colonization, therefore, represents an important approach to limit VRE transmission. We demonstrate that reintroduction of a diverse intestinal microbiota to densely VRE colonized mice eliminates VRE from the intestinal tract. While oxygen-tolerant members of the microbiota are ineffective at eliminating VRE, administration of obligate anaerobic commensal bacteria to mice results in a billion-fold reduction in the density of intestinal VRE colonization. 16S rRNA gene sequence analysis of intestinal bacterial populations isolated from mice that cleared VRE following microbiota reconstitution revealed that re-colonization with a microbiota that contains Barnesiella correlates with VRE elimination. Characterization of the fecal microbiota of patients undergoing allogeneic hematopoietic stem cell transplantation demonstrated that intestinal colonization with Barnesiella confers resistance to intestinal domination and bloodstream infection with VRE. Our studies indicate that obligate anaerobic bacteria belonging to the Barnesiella genus enable clearance of intestinal VRE colonization and may provide novel approaches to prevent the spread of highly antibiotic-resistant bacteria.

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Available from: Lauren Lipuma, Sep 30, 2015
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    • "The protective effect of some bacterial species such as Barnesiella has been also confirmed in cases of broad antibiotic treatment [9] "
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    • "16S rRNA Gene Amplification, Pyrosequencing, and Analysis For each sample, the V1–V3 region of the 16S rRNA gene was amplified by PCR and sequenced using a 454 GS FLX Titanium platform following Roche recommendations. Sequences were processed using mothur (Schloss et al., 2009) as previously described (Ubeda et al., 2013), with some modifications. See Supplemental Experimental Procedures for further details of all methods and statistical analyses. "
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