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Problem statement: At present, the novel agent that is effective, cheap and easy to approach for treating male sexual dysfunction is required due to the current poor therapeutic efficacy. Though Moringa oleifera is reputed for aphrodisiac activity in traditional folklore, no scientific evidence is available. Therefore, we aimed to determine the effect of M.oleifera leaves extract on male sexual behaviors in animal model of sexual dysfunction. Moreover, the possible underlying mechanisms were also investigated. Approach: Male Wistar rats, weighing 200-250 g, had been orally given M.oleifera leaves extract at doses of 10, 50 and 250 mg kg −1 BW once daily at 30 min before the exposure to 12-h immobilization stress for 14 days. They were assessed male sexual behaviors including mounting, intromission and ejaculation numbers and latencies after single administration and every 7 days until the end of experiment. To further investigate the possible mechanisms of action, we also determined serum testosterone level of all rats at the end of experiment together with the determination of suppression effect of the plant extract on MAO B and PDE-5 activities. Results: The results showed that after single administration, rats subjected to M.oleifera extract at dose of 10 mg kg −1 BW significantly enhanced mounting number. When the treatment was prolonged to 7 days rats subjected to the low dose of extract showed the enhanced intromission number whereas rats subjected to high dose of extract showed the enhanced mounting number. Our data also showed no significant change in serum testosterone level. However, the extract could also suppress MAO B and PDE-5 activities. Taken all together, the extract could enhance male sexual desire and performance via the suppression of MAO B and PDE-5 activities. Conclusion: M.oleifera can be the potential sexual enhancer particularly for acute and short term application. However, further researches are necessary.
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... However, heat stress may affect the number of mature follicles (Naseer et al., 2017) hence the decrease in progesterone secretion because of less number of corpus luteum sites as was noticed in the control. Vitamin antioxidants have been known to alleviate heat stress in rabbits (Prabsattroo et al., 2012(Prabsattroo et al., , 2015Anoh 2017;El-Desoky, et al., 2017). The presence of these phyto-chemical compounds may facilitate the ability of animals to rapidly grow follicles and shed more ova, creating more corpus luteum (CL) sites and increasing progesterone by counteracting free radicals and other reactive oxygen (ROS) species production in body fluids and tissues due to heat stress. ...
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... People believe that certain medicinal plants have aphrodisiac properties that could benefit male reproductive health (Singh et al., 2010;Malviya et al., 2011). Moringa oleifera (Family: Moringaceae) is a well-known medicinal plant that has been used traditionally due to its antimicrobial (Rahman et al., 2010;Elgamily et al., 2016), antihyperglycemic (Tuorkey, 2016;Paula et al., 2017), anticancer (Gismondi et al., 2013), aphrodisiac (Suresh et al., 2009;Prabsattroo et al., 2012;Prabsattroo et al., 2015;Shokry et al., 2020), and anti-inflammation (Adedapo et al., 2015;Arulselvan et al., 2016) properties. Its leaves contain a high concentration of zinc, potassium, phosphorus, selenium, calcium, vitamin A, vitamin C, vitamin D, and essential amino acids (Mahmood et al., 2010;Mishra et al., 2011). ...
... The oral administration of Moringa oleifera leaves extract showed more persistent sexual activity compared to other control groups. According to Prabsattroo et al. (2012) and Prabsattroo et al. (2015), Moringa oleifera leaves extract can improve sexual performance. The increase in ML may be associated with sexual motivation while the decrease in MF may reflect the sexual desire and performance of the treated group (Yakubu et al., 2011;Kataria et al., 2013). ...
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... The extracts were stored in a dark bottle at À4°C until used. The crude extract was suspended in distilled water Prabsattroo et al., 2012) and by using standard methods according to (Trease and Evans, 1972) Phytochemical analyses of the extracts were performed by Elangovan (2014). ...
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This study examined the behavioral responses, purinergic receptor densities, ectonucleotidases (E-NTPDase), adenosine deaminase (ADA) activity, and nitric oxide (NO) levels along with the parameters of oxidative stress–related to erectile function in the cerebral cortex (CC) of L-NAME-challenged rats pretreated with tigernut (TN) and walnut (WN) dietary supplementation. Wistar rats (male) of 70 total animals (250–300 g) were used in this research and hence separated into seven groups (n = 10): Group I: normal control-fed basal diet; Group II: positive control-fed basal diet/L-NAME/Sildenafil citrate (5 mg kg⁻¹ day⁻¹); Group III: ED-induced (placed on a basal diet/L-NAME); Group IV: diet supplemented with processed TN (20%)/L-NAME; Group V: diet supplemented with raw TN (20%)/L-NAME; Group VI: diet supplemented with processed WN (20%)/L-NAME; and Group VII placed on a diet supplemented with raw WN (20%)/L-NAME. The rats were pretreated for 2 weeks before the L-NAME (40 mg kg⁻¹ day⁻¹) challenge on their respective diet. L-NAME brought about a decrease in the sexual behaviors evaluated while the effect was significantly reversed by supplemented diets containing TN and WN. L-NAME increased the levels of reactive oxygen species and malondialdehyde, E-NTPDase as well as ADA activities, and caused the level of NO in the CC as well as the purinoreceptor densities to be downregulated. Treatments with enriched diets, however, greatly reverse these effects. The behavioral responses and neuromodulatory capacity of the nuts displayed on the CC can, therefore, further support their aphrodisiac property. Practical applications The results revealed the ability of tigernut (TN; Cyperus esculentus L.) and walnut (WN; Tetracarpidium conophorum Müll. Arg.) to enhance behavioral responses; modulate purinergic receptor densities, E-NTPDase, and ADA activities; increase NO levels; and prevent oxidative stress related to erectile function in the CC of L-NAME-challenged Wistar rats. The results show that these nuts are useful feeds for both animal and human nutrition.
... The same thing has been previously reported by Rachmawati et al. [3] that there is a positive correlation between the concentrations of testosterone on the level of libido, the higher the concentration of testosterone, the higher the level of libido. It is further explained that libido is influenced by the concentration of testosterone [24,25]. ...
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Massularia acuminata stem is often used in folkloric medicine in the management of erectile dysfunction (ED), without full scientific basis for its action. Thus, the effects of aqueous extract of M. acuminata stem (MAS) on sexual activity, hormonal action, enzymatic activity and levels of molecules associated with erectile function were assessed. ED was induced by single intraperitoneal injection of 50 mg/kg body weight of streptozotocin in rats and treated with sildenafil citrate or MAS (50 or 100 mg/kg) orally for 2 weeks. The results revealed that there was significant (p < 0.05) reduction in mounting and intromission frequencies, testosterone, luteinizing hormone, and nitric oxide levels, as well as elevation in mounting and intromission latencies, phosphodiesterase 5, arginase, acetylcholinesterase, adenosine triphosphatidase, and adenosine deaminase activities, nitric oxide, thiobarbituric acid reactive species, and glycated haemoglobin levels were observed in ED rats in comparison with the control rats. Treatment with MAS or sildenafil citrate significantly (p < 0.05) modulated the sexual behaviour, biochemical parameters and histological architecture, with 100 mg/kg of MAS having the best erectogenic effects. Furthermore, phenolic characterization revealed that catechin and kaempferol as the main phenolic compounds present in MAS, that can act in synergistically or additively with other phytochemicals to confer erectogenic effect.
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