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Sugar components of coconut sugar in Indonesia

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ASEAN Food Journal Vol. 14, 45-49
Volatile Components of Coconut Fresh Sap, Sap Syrup and Coconut Sugar 45
ASEAN Food Journal 14 (1): 45-49 (2007)
Volatile Components of Coconut Fresh Sap,
Sap Syrup and Coconut Sugar
Purnomo, H.
Department of Animal Food Science and Technology,
Faculty of Animal Husbandry, Brawijaya University
Malang, East Jawa, Indonesia
Abstract: Volatile components of coconut fresh sap, sap syrup and coconut sugar were analyzed
using GC-MS and identified by matching the mass spectra obtained with those present in the
“NIST62 LIB” Library. The most abundant volatile components in the fresh sap, sap syrup and
coconut sugar were 2-butanol and acetic acid. Twelve volatile components were identified and
there were different amounts of volatile components found in the morning and afternoon tapping
of fresh sap and its processed products.
Keywords: Volatile components, coconut fresh sap, sap syrup, coconut sugar
INTRODUCTION
Coconut sap, known as nira (Indonesia), toddy
(Sri Lanka), maprau (Thailand), lagbi (North
Africa), is the raw material in the production
of coconut sugar (Nathanael, 1970;
Anonymous, 1980; Purnomo, 1992). Coconut
sugar in Indonesia is produced at cottage level
and the indigenous production technology of
coconut sugar has been reviewed by Hori et al.
(2001a) and Purnomo and Suryoseputro
(2001).
Furthermore, Purnomo (1992) and
Purnomo and Suryoseputro (2001) reported
that coconut sugar has been widely used as an
ingredient in traditional Indonesian cooking,
food and beverages. It is also one of the major
ingredients of Indonesian sweet soya sauce
(kecap manis), as well as in the Indonesian
intermediate moisture meat (dendeng). The
coconut sugar gives a specific taste and flavour
to the end products.
Apriyantono et al. (1996) reported that
some of the volatile components typical of
Mailland reaction products found in sweet soya
sauce apparently originated from coconut
sugar as one of the main ingredients. They also
predicted that the volatile components derived
from the coconut sap, as well as during it’s
processing into coconut sugar.
In view of the specific flavour of coconut
sugar, which makes it a special ingredient in
some Indonesian cuisines, snacks, beverages
and sweet soya sauce, it was of interest to
investigate the volatile components of fresh
sap, sap syrup and coconut sugar.
MATERIALS AND METHODS
Materials
Fresh sap, sap syrup and coconut sugar
samples were obtained from the coconut
plantation and coconut sugar processor in
Srengat village, Blitar, East Java. The samples
were taken at random after morning and
afternoon tapping, and then processed into
sap syrup (75
o
Brix) and coconut sugar.
Sodium metabisulphite of 1000 ppm
concentration was added as preservative
during the processing of fresh sap into syrup
and coconut sugar.
E-mail address: purnomo.hari@lycos.com (Purnomo, H.)
ASEAN Food Journal Vol. 14, 45-49
46 Purnomo, H.
Methods
Volatile components of fresh sap (50 ml), sap
syrup (50 ml) and coconut sugar (5 g in 50 ml
distilled water) were extracted using Likens-
Nickerson apparatus with diethylether as the
extraction solvent. The extracts were dried
with anhydrous sodium sulphate and then
concentrated using a rotary evaporator
followed by flushing using nitrogen until the
volume was about 0.5 ml (Apriyantono
et al., 1996).
The GC-MS analyses of the extracts were
performed using a set of GC-MS Shimadzu QP-
5000 (Japan) with a CBP-5 column of 50 m at
temperatures of 80
o
- 250
o
(10
o
C/minutes).
The injector temperature was 260
o
C with mode
split 1 : 60. Helium was used as a carrier gas at
a pressure of 14 Kpa and the ion source
working in electron impact(EI) mode at 70 eV
was held about 180
o
C (Alli et al., 1990;
Apriyantono et al., 1996).
The identification was done by matching
the mass spectra obtained with those present
in the “NIST62.LIB” Library. It was then
confirmed by matching their Linear Retention
Indices (LRI) values with those already
published in the literature. The internal
standard used for quantification of volatile
using GC-MS was 1,4 – dichlorobenzene
(Apriyantono et al., 1996).
RESULTS AND DISCUSSION
Results
The major volatile components identified in
the fresh sap, sap syrup and coconut sugar
were 2-butanol and acetic acid. Dodecanoic
acid was found in sap syrup and coconut sugar,
whilst 1,4 dimethyl-6,1-butyl acetate was found
in smaller amounts. N,N dimethyl-2-(diphenyl-
methoxy)-ethylamine and methylpyrazine
were identified in coconut sugar only. In fresh
sap, 2 methylcyclohexane and cyclohexiloc-
tane are volatile components that were found
only in fresh sap. Only twelve volatile
components were found in fresh sap, sap syrup
and coconut sugar from Srengat village, Blitar,
East Jawa.
Acetic acid was identified both in the
morning and afternoon fresh sap, sap syrup
and coconut sugar produced from these fresh
sap. Some of the volatile components in the
fresh sap decreased in concentration and in
some cases not detectable in the sap syrup and
coconut sugar. 2-butanol in fresh sap for
example was in the range of 60.26 – 68.37%
but decreased in the sap syrup to between
45.35 and 51.02%, and in the coconut sugar it
was reduced to as much as 29.98 – 31.23%.
Discussion
In this study, only 12 volatile components were
identified, whilst Apriyantono et al. (1996)
reported that around 70 volatile components
were identified in coconut sugar. They also
noted that the major volatile components in
coconut sugar were dodecanoic acid, acetic
acid, 2-undecanone-decanoic acid, 2-
nonanone and 2-furfural. It was assumed that
those components were derived from the
coconut sap and many of which are formed
during the cooking of fresh sap for about four
hours to produce coconut sugar.
The differences in volatile components
identified in the coconut sugar samples in this
study compared to that of Apriyantono et al.
(1996) is possibly due to the different
composition of the fresh sap used as raw
material to produce the coconut sugar in
addition to the possible difference of cooking
methods. Hori et al. (2001b) reported that the
composition of the substances in fresh sap
varied depending on the coconut variety, stage
of maturity of the inflorescence of coconut
tree, climatic condition as well as soil fertility
status.
It is also assumed that the differences of
both natural (such as jack fruit bark or
mangosteen fruit skin) and chemical
preservatives (such as sodium metabisuphite
or limestone (Ca(OH)
2
) solution) used during
sap tapping also affected the components of
fresh sap syrup and coconut sugar. The heating
ASEAN Food Journal Vol. 14, 45-49
Volatile Components of Coconut Fresh Sap, Sap Syrup and Coconut Sugar 47
Table 1: Volatile components of fresh sap, sap syrup and coconut sugar
Components A (%) B(%) C (%)
121212
Acetic acid 30.43 25.83 26.47 24.56 21.54 35.05
Dodecanoic acid nd nd 0.34 21.59 nd 12.41
1,4 dimethyl-6-1. butyl-acetate 1.11 0.91 10.26 0.40 15.50 1.76
2 methylcyclohexane 6.39 0.66 nd nd nd nd
Cyclohexiloctane 1.81 4.23 nd nd 17.01 nd
2-butanol 60.26 68.37 51.02 45.35 31.23 29.98
N,N dimethyl 2-(diphenylmetoxi)-ethylamine nd nd nd nd 13.26 9.31
Methylpyrazine nd nd nd nd 1.46 1.81
2,3 dimethylpirazine nd nd 0.77 nd nd nd
4,6 dimethyl-5-cyclo-hexo pyrimidine nd nd nd 2.25 nd nd
2-Furan nd nd 6.73 1.97 nd nd
Cyclohexane nd nd 4.41 3.56 nd nd
A
1
fresh sap tapped in the morning
A
2
fresh sap tapped in the afternoon
B
1
sap syrup (75Brix) preserved with 1000 ppm Na
2
S
2
O
5
obtained from morning tapped fresh sap
B
2
sap syrup (75Brix) preserved with 1000 ppm Na
2
S
2
O
5
obtained from afternoon tapped fresh sap
C
1
coconut sugar made from sap syrup B
1
C
2
coconut sugar made from sap syrup B
2
nd : not detected
ASEAN Food Journal Vol. 14, 45-49
48 Purnomo, H.
method, temperature and time of boiling are
also assumed to affect the quality of sap syrup
and coconut sugar. Widyaningsih (1983),
Purnomo (1992) and Sumarmin (1994) also
reported the effect of different natural and or
chemical preservatives used during fresh sap
collection on the quality of coconut sugar in
terms of flavor, taste, texture and color.
The reduction in the amount of some
volatile components found in the sap syrup
and coconut sugar was possibly due to the heat
treatment and the possible interaction
between sugar components and amino acids
in fresh sap during processing. The
mechanisms of changes are not fully
understood and hence further studies are
needed. Alli et al. (1990) and Akochi et al.
(1997) reported that methyl-; 2,6 dimethyl-;
ethyl; 2,3 dimethyl- and 2-ethyl 3-
methylpyrazine were detected after heating
maple syrup at 105
o
C for 120 minutes.
Furthermore, Akochi et al. (1997) also noted
that the presence of sucrose, fructose and trace
amounts of amino acids were the precursors
that participate in the formation of
alkylpyrazines in foods.
Fresh sap of coconut contains a small
amount of protein, fat, minerals and vitamins
as well as sugar components. These could
possibly interact during heat processing and
form some of the volatile components and
non-enzymatic browning intermediates as well
as Maillard products. Fernandez (1983), as
cited by Jatmika et al. (1990) found glutamic
acid, threonine, aspartic acid and serine as
major amino acids in fresh sap, whilst proline,
methionine, triptophane, and histidine were
< 4.00mg/100 g fresh sap. Itoh et al. (1982)
reported that fresh sap of coconut contain
sucrose, ash, protein, vitamin C and acids,
such as succinic acid, and citic acid.
Therefore, the sap syrup and coconut
sugar has the capacity as a sweetener with an
enjoyable caramel and exquisite coconut
flavor. The distinct role of volatile components
which gave a blend of caramel and coconut
flavor distinguished the sap syrup and coconut
sugar from cane sugar, caramel, maple syrup
or other sugar caramels.
CONCLUSION
The volatile components identified in fresh
sap, sap syrup and coconut sugar were 2-
butanol and acetic acid. There were 12 volatile
components identified in fresh sap, sap syrup
and coconut sugar from this region.
ACKNOWLEDGEMENT
Special thanks to Ms Lutfi Hamidah for
assistance in laboraotory analysis of the
samples.
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ASEAN Food Journal Vol. 14, 45-49
Volatile Components of Coconut Fresh Sap, Sap Syrup and Coconut Sugar 49
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... Since the contaminated sap emits a putrid smell, it cannot be treated as pure fruit juice. However, it can be processed into value-added products [26]. ...
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  • S Sur Yoseputro
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Compositional of nira palm juice of high sugar content from palm tree
  • T Itoh
  • A Matsuyama
  • C H Widjaya
  • M Z Nasution
  • J Kumendong
Itoh, T., Matsuyama, A., Widjaya, C.H., Nasution, M.Z. and Kumendong, J. 1982. Compositional of nira palm juice of high sugar content from palm tree. Proceedings of IPB-JICA International Symposium on Agricultural Product, Processing and Technology.
Coconut processing technology information documents parts 5 of 7. Domestic Coconut Food Processes
  • Anonymous
Anonymous. 1980. Coconut processing technology information documents parts 5 of 7. Domestic Coconut Food Processes, p. 152-170. Austria: United Nations Industrial Development Organization (UNIDO).
Coconut toddy tapping and cottage manufacture of treacle and jaggery
  • W R W Nathanael
Nathanael, W.R.W. 1970. Coconut toddy tapping and cottage manufacture of treacle and jaggery. Ceylon Coconut Planters Review, 6: 63-65.
Alternatif produk olahan dari nira kelapa (Alternative processed coconut sap products)
  • A Jatmika
  • A Mahlil-Hamzah
  • D Siahaan
Jatmika, A., Mahlil-Hamzah, A. and Siahaan, D. 1990. Alternatif produk olahan dari nira kelapa (Alternative processed coconut sap products). Buletin Kelapa Manggar, 3 (3): 37-57.
Mempelajari jenis bahan pengawet nira dan cara pembungkusan terhadap mutu gula kelapa (Cocos nucifera Linn) selama penyimpanan. (Study on the effect of different kind of sap preservatives and the wrapping method on the quality of coconut (Cocos nucifera Linn) sugar during storage)
  • A Widyaningsih
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Identification of pyrazines in mapple syrup
  • L Alli
  • J Bourque
  • R Metussin
  • R Liang
  • Yaylalayan
Alli, L., Bourque, J., Metussin, R., Liang, R. and Yaylalayan. 1990. Identification of pyrazines in mapple syrup. Journal of Agriculture Food Chemistry, 38: 1242-1244.
Indigenous technology of coconut sugar production in the village of Genteng, Banyuwangi (East Jawa) and Dawan, Klungkung (Bali) and the knowledge about palm sugar by Japanese young people
  • K Hori
  • S Suryoseputro
  • H Purnomo
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