As the result of a collecting trip to the peninsula of Chalkidiki in NE Greece in autumn 2002, 169 bryophyte taxa were recognized. Three of these, Ephemerum serratum, Riccia beyrichiana and Thuidium delicatulum, are new records for Greece, six are new records for mainland Greece, 23 for NE Greece and 19 for Chalkidiki. The high percentage of new records (31 %) demonstrates the poor state of the bryological exploration of Greece. An annotated catalogue of the collected taxa is presented, including notes on substrates and associated bryophytes.