Infection by chikungunya virus modulates the expression of several proteins in Aedes aegypti salivary glands

Parasites & Vectors (Impact Factor: 3.43). 11/2012; 5(1):264. DOI: 10.1186/1756-3305-5-264
Source: PubMed


Arthropod-borne viral infections cause several emerging and resurging infectious diseases. Among the diseases caused by arboviruses, chikungunya is responsible for a high level of severe human disease worldwide. The salivary glands of mosquitoes are the last barrier before pathogen transmission.

We undertook a proteomic approach to characterize the key virus/vector interactions and host protein modifications that occur in the salivary glands that could be responsible for viral transmission by using quantitative two-dimensional electrophoresis.

We defined the protein modulations in the salivary glands of Aedes aegypti that were triggered 3 and 5 days after an oral infection (3 and 5 DPI) with chikungunya virus (CHIKV). Gel profile comparisons showed that CHIKV at 3 DPI modulated the level of 13 proteins, and at 5 DPI 20 proteins. The amount of 10 putatively secreted proteins was regulated at both time points. These proteins were implicated in blood-feeding or in immunity, but many have no known function. CHIKV also modulated the quantity of proteins involved in several metabolic pathways and in cell signalling.

Our study constitutes the first analysis of the protein response of Aedes aegypti salivary glands infected with CHIKV. We found that the differentially regulated proteins in response to viral infection include structural proteins and enzymes for several metabolic pathways. Some may favour virus survival, replication and transmission, suggesting a subversion of the insect cell metabolism by arboviruses. For example, proteins involved in blood-feeding such as the short D7, an adenosine deaminase and inosine-uridine preferring nucleoside hydrolase, may favour virus transmission by exerting an increased anti-inflammatory effect. This would allow the vector to bite without the bite being detected. Other proteins, like the anti-freeze protein, may support vector protection.

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Available from: Abdelkader Namane, Dec 16, 2013
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    • "Nevertheless, advances in genome sequencing throughput and technology have facilitated the examination of individual mosquito genes in a truly quantitative way. To date, there have been many studies examining vector proteomics and gene expression as they pertain to infection (Behura et al., 2011; Bonizzoni et al., 2012; Chen, Mathur, & James, 2008; Colpitts et al., 2011; Girard et al., 2010; Tchankouo-Nguetcheu et al., 2012, 2010). One in which the authors analyzed transcriptome expression of Cx. quinquefasciatus, Ae. aegypti, and Anopheles gambiae in response to infection with WNV, Wuchereria bancrofti, and nonnative bacteria demonstrated the utility of such methods to generate unparalleled amounts of quantitative data for analysis. "
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    • "The most frequently used technique in quantitative proteomics is two-dimensional electrophoresis (2-DE). In quantitative 2-DE, the appropriate experimental design plays an important role in the detection of significant and reliable protein expression differences [12]. Despite that there are some limitations of the technology, such as offering a limited dynamic range of separated proteins, it has been used to investigate host cell proteome changes after infection with T. gondii tachyzoites [13,14], but little is currently known of the proteomic changes at differential time points in host brain tissues after infection with T. gondii cysts. "
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