ZHDANOV,VICTORM. (TheD. I. Ivanovsky Institute ofVirology, Moscow, USSR),NONNAB.AZADOVA, AND LEONIDV.URYVAYEV. Topography anddynamics ofsynthesis ofstructural proteins ofNewcastle disease virus. J.Bacteriol. 91:1902- 1906. 1966.-Newcastle disease virus SandV antigens aresynthesized inthecyto- plasm, asrevealed bytheimmunofluorescence method. Insome experiments, S antigen was
... [Show full abstract] foundalsointhenucleoli. Actinomycin D moderately decreased the titer ofinfectious virus andV antigen andaccelerated thetimeofappearanceof maturevirus. Proflavine sharply decreased thesynthesis ofbothantigens andthe release ofmaturevirus. Previous studies ofNewcastle disease virus (NDV)haveshownthatbothprotein compo- nents, theSandVantigens, aresynthesized inthe cytoplasm (4). Whetherthevirus undergoes a complete oranabortive cycle ofmultiplication depends uponthenature ofthehostcell. Inthe papercited thesites ofvirus protein synthesis wereexamined by immunofluorescence, em- ploying SandV antisera obtained bythemethod ofLiefandHenle(3). Thismethod, however, doesnotexclude cross- reactions between SandV components. There- fore, weconsidered itworthwhile tostudy the topography anddynamics ofthesynthesis of structural proteins ofNDV byuseofhighly puri- fied SandVantisera. Inaddition, theinfluence of actinomycin D andproflavine onvirus synthesis wasinvestigated. Actinomycin D inhibits thesyn- thesis ofdeoxyribonucleic acid (DNA)-dependent ribonucleic acid(RNA);proflavine reacts with bothDNA andRNA (6). Itwashoped thatstudy ofthereproduction ofNDV underthese condi- tions wouldshednewlight onthesynthesis of structural proteins andtheformation ofthema- turevirus.