Article

The carbohydrate-active ENZYMES database (CAZy): an expert resource for glycogenomics. Nucleic Acids Res 37:D233-D238

Architecture et Fonction des Macromolécules Biologiques, UMR6098, CNRS, Universités Aix-Marseille I & II, 163 Avenue de Luminy, 13288 Marseille, France.
Nucleic Acids Research (Impact Factor: 9.11). 11/2008; 37(Database issue):D233-8. DOI: 10.1093/nar/gkn663
Source: PubMed

ABSTRACT

The Carbohydrate-Active Enzyme (CAZy) database is a knowledge-based resource specialized in the enzymes that build and breakdown
complex carbohydrates and glycoconjugates. As of September 2008, the database describes the present knowledge on 113 glycoside
hydrolase, 91 glycosyltransferase, 19 polysaccharide lyase, 15 carbohydrate esterase and 52 carbohydrate-binding module families.
These families are created based on experimentally characterized proteins and are populated by sequences from public databases
with significant similarity. Protein biochemical information is continuously curated based on the available literature and
structural information. Over 6400 proteins have assigned EC numbers and 700 proteins have a PDB structure. The classification
(i) reflects the structural features of these enzymes better than their sole substrate specificity, (ii) helps to reveal the
evolutionary relationships between these enzymes and (iii) provides a convenient framework to understand mechanistic properties.
This resource has been available for over 10 years to the scientific community, contributing to information dissemination
and providing a transversal nomenclature to glycobiologists. More recently, this resource has been used to improve the quality
of functional predictions of a number genome projects by providing expert annotation. The CAZy resource resides at URL: http://www.cazy.org/.

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Available from: Brandi L Cantarel
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    • "Several different approaches can be employed to understand and predict the selective influence of ND carbohydrates upon the gut microbiota. Analysis of carbohydrate active enzyme (CAZyme, Carbohydrate Active Enzymes database[16,17], URL http://www.cazy.org/) complements from individual genomes[18]and growth tests on isolated species[19]may be indicative, but cannot predict how different organisms will compete and interact within the complex intestinal community. "
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    • "In addition to cellulases, degradation of plant cell walls requires pectinases and hemicellulases. These are all grouped into the glycoside hydrolase (GH) family according to their amino acid sequence similarities and their folding patterns based on the Carbohydrate-Active enZymes Database[82]. Cellulases in the GH9 family are found in most insect orders while polygalacturonases of the GH28 family have a much more restricted distribution in insects[83]. Homogalacturonan polymers are the main components of pectin in primary cell walls, and the polygalacturonases identified in this study presumably cleave the 1,4-linkages of the homogalacturonan α-D-galacturonic acid[83]. "
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    • "At present, 133 glycoside hydrolase (GH) families are listed in the frequently updated Carbohydrate Active enZYme (CAZY) website (http://www.cazy.org) (Cantarel et al. 2009; Cairns and Esen 2010). These families are further classified into clans. "
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